Abstract:
:We have recently shown that phosphorothioate (PS) oligodeoxynucleotide (ODN) analogs, unlike their normal congeners, exhibit significant anti-HIV activity (Matsukura et al., (1987) Proc. Natl. Acad. Sci. USA 84, 7706-7710). We now report the syntheses, melting temperatures (Tm), and nuclease susceptibilities of a series of phosphorothioate ODN analogs. These include all-PS duplexes, duplexes with one normal chain and the other chain either all-PS, or end-capped with several PS groups at both 3' and 5' ends. The DNase susceptibilities of the S-ODNs are much less than the normal phosphodiesters, but by contrast duplexes of poly-rA with S-dT40 are much more susceptible to RNase H digestion. The Tm's for AT base pairs of S-ODNs are significantly depressed relative to normals, while GC base pairs show much less Tm depression. The Tm's of S-dT oligomers with poly-rA are reduced relative to the duplexes with normal dA oligomers. These results have significance for the biological properties of these analogs as anti-message inhibitors of gene expression, and provide a rational basis for the S-dC/G sequences as potential effective anti-AIDS agents.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Stein CA,Subasinghe C,Shinozuka K,Cohen JSdoi
10.1093/nar/16.8.3209subject
Has Abstractpub_date
1988-04-25 00:00:00pages
3209-21issue
8eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::The transfer ribonucleic acids (tRNAs) of B. subtilis at different growth phases are examined for changes in the composition and the methylation of minor constituents. The composition of the tRNAs indicates about equal amounts of adenosine and uridine, and of guanosine and cytidine. About 3-4 residues are present as m...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:1982-04-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkr1293
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.21.e103
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-12-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-01-23 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2004-09-23 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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