Abstract:
:A 12 bp long GCN4-binding, self-complementary duplex DNA d(CATGACGTCATG)(2) has been investigated by NMR spectroscopy to study the structure and dynamics of the molecule in aqueous solution. The NMR structure of the DNA obtained using simulated annealing and iterative relaxation matrix calculations compares quite closely with the X-ray structure of ATF/CREB DNA in complex with GCN4 protein (DNA-binding domain). The DNA is also seen to be curved in the free state and this has a significant bearing on recognition by the protein. The dynamic characteristics of the molecule have been studied by (13)C relaxation measurements at natural abundance. A correlation has been observed between sequence-dependent dynamics and recognition by GCN4 protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Khandelwal P,Panchal SC,Radha PK,Hosur RVdoi
10.1093/nar/29.2.499keywords:
subject
Has Abstractpub_date
2001-01-15 00:00:00pages
499-505issue
2eissn
0305-1048issn
1362-4962journal_volume
29pub_type
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