Abstract:
:A rearrangement of the mitochondrial genome of a long lived mutant of Podospora anserina is presented. It consists in the insertion of 191 bp of the LrDNA gene (coding for the large ribosomal RNA) at the junction between exon1 and intron alpha of gene co1 (coding for subunit 1 of cytochrome oxidase). This insertion is accompanied by a 53 bp deletion of the junction and the presence of extra A and T nucleotides at both sides of the inserted sequence. We discuss possible mechanisms of production of this rearrangement. The presence of extra nucleotides at the recombination junctions suggests that it may pass through a stage of free DNA ends originating from a DNA break at the junction between exon1 and intron alpha of gene co1. The possibility that such a DNA break plays a major role in the instability of the mitochondrial genome is envisaged.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sainsard-Chanet A,Begel Odoi
10.1093/nar/18.4.779subject
Has Abstractpub_date
1990-02-25 00:00:00pages
779-83issue
4eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract::The dissection of RNA editing mechanisms in PHYSARUM: mitochondria has been hindered by the absence of a soluble in vitro system. Based on our studies in isolated mitochondria, insertion of non-encoded nucleotides into PHYSARUM: mitochondrial RNAs is closely linked to transcription. Here we have fractionated mitochond...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.19.3695
更新日期:2000-10-01 00:00:00
abstract::The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated reaction during transposition. In this study, comparative DNA-binding analyses were performed using purified full-length Tnp and a C-terminal deletion variant (delta369) that lacks the putative dimerization domain. The shape of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.19.3790
更新日期:1996-10-01 00:00:00
abstract::The TPA-inducible transcription factor AP-1, consisting of homo- or hetero-dimers of members of the Jun- and Fos-families, regulates transcription of a wide variety of genes containing the TPA response element (TRE). In P19 embryonal carcinoma (EC) cells, Jun D is the only component of AP-1 expressed, while in these c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.1.125
更新日期:1992-01-11 00:00:00
abstract::Arguably, the most bizarre mitochondrial DNA (mtDNA) is that of the euglenozoan eukaryote Diplonema papillatum. The genome consists of numerous small circular chromosomes none of which appears to encode a complete gene. For instance, the cox1 coding sequence is spread out over nine different chromosomes in non-overlap...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq883
更新日期:2011-02-01 00:00:00
abstract::We have sequenced the entire human S mu and S gamma 4 immunoglobulin heavy chain class switch regions, and have also completed the sequence of human S epsilon. S mu is composed predominantly of GAGCT and GGGCT pentameric repeats, with these units also being found in S epsilon at a much lower density. S mu-S gamma 4 ma...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7305
更新日期:1990-12-25 00:00:00
abstract::Escherichia coli leucyl-tRNA synthetase (LeuRS) aminoacylates up to six different class II tRNA(leu) molecules. Each has a distinct anticodon and varied nucleotides in other regions of the tRNA. Attempts to construct a minihelix RNA that can be aminoacylated with leucine have been unsuccessful. Herein, we describe the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.10.2103
更新日期:2002-05-15 00:00:00
abstract::A method is described to simultaneously amplify and sequence DNA using a new class of nucleotides containing boron. During the polymerase chain reaction, boron-modified nucleotides, i.e. 2'-deoxynucleoside 5'-alpha-[P-borano]-triphosphates, are incorporated into the product DNA. The boranophosphate linkages are resist...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.8.1611
更新日期:1997-04-15 00:00:00
abstract::PipeAlign is a protein family analysis tool integrating a five step process ranging from the search for sequence homologues in protein and 3D structure databases to the definition of the hierarchical relationships within and between subfamilies. The complete, automatic pipeline takes a single sequence or a set of sequ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg518
更新日期:2003-07-01 00:00:00
abstract::As a trial practical application, we have applied optimized microfabricated electrophoresis devices, combined with enzymatic mutation detection methods, to the determination of single nucleotide polymorphism (SNP) sites in the p53 suppressor gene. Using clinical samples, we have achieved robust assays with quality fac...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.9.e43
更新日期:2000-05-01 00:00:00
abstract::The nucleotide sequence of Neurospora crassa 5.8 S rDNA and adjacent regions has been determined. The deduced 5.8 S rRNA sequence of Neurospora differs from the 5.8 S rRNA sequence of Saccharomyces cerevisiae at 13 of 158 residues. Nine of these differences are clustered in a segment capable of forming a short hairpin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.7.2561
更新日期:1979-06-11 00:00:00
abstract::Accurate maps and DNA sequences for human subtelomere regions, along with detailed knowledge of subtelomere variation and long-range telomere-terminal haplotypes in individuals, are critical for understanding telomere function and its roles in human biology. Here, we use a highly automated whole genome mapping technol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx017
更新日期:2017-05-19 00:00:00
abstract::The DNA Data Bank of Japan (DDBJ) (http://www.ddbj.nig.ac.jp) has been providing public data services for thirty years (since 1987). We are collecting nucleotide sequence data from researchers as a member of the International Nucleotide Sequence Database Collaboration (INSDC, http://www.insdc.org), in collaboration wi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1001
更新日期:2017-01-04 00:00:00
abstract::DNA predominantly contains Watson-Crick (WC) base pairs, but a non-negligible fraction of base pairs are in the Hoogsteen (HG) hydrogen bonding motif at any time. In HG, the purine is rotated ∼180° relative to the WC motif. The transitions between WC and HG may play a role in recognition and replication, but are diffi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz837
更新日期:2019-12-02 00:00:00
abstract::Rice alpha-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.23.7007
更新日期:1990-12-11 00:00:00
abstract::Targeted delivery of antisense oligonucleotides (ASO) to hepatocytes via the asialoglycoprotein receptor (ASGR) has improved the potency of ASO drugs ∼30-fold in the clinic (1). In order to fully characterize the effect of GalNAc valency, oligonucleotide length, flexibility and chemical composition on ASGR binding, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx060
更新日期:2017-03-17 00:00:00
abstract::DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.21.6597
更新日期:1982-11-11 00:00:00
abstract::Spliceosomes are assembled in stages. The first stage forms complex E, which is characterized by the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice site and proteins thought to connect them. The splice sites are held in close proximity and the pre-mRNA is committed to spl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks322
更新日期:2012-08-01 00:00:00
abstract::The genomic distribution of the abundant eukaryotic d(GA x TC)(n) DNA microsatellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombination in SV40 minichromosomes, the rate of homologous DNA recombination increasing by as much...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.23.4617
更新日期:2000-12-01 00:00:00
abstract::A long-range physical map of the centromeric region of human chromosome 7 has been constructed in order to define the region containing sequences with potential involvement in centromere function. The map is centered around alpha satellite DNA, a family of tandemly repeated DNA forming arrays of hundreds to thousands ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.9.2295
更新日期:1991-05-11 00:00:00
abstract::Signaling pathways constitute a valuable source of information that allows interpreting the way in which alterations in gene activities affect to particular cell functionalities. There are web tools available that allow viewing and editing pathways, as well as representing experimental data on them. However, few metho...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt451
更新日期:2013-07-01 00:00:00
abstract::DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1303
更新日期:2014-03-01 00:00:00
abstract::Decay of (125)I produces a shower of low energy electrons (Auger electrons) that cause strand breaks in DNA in a distance-dependent manner with 90% of the breaks located within 10 bp from the decay site. We studied strand breaks in RNA molecules produced by decay of (125)I incorporated into complementary DNA oligonucl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkf622
更新日期:2002-11-15 00:00:00
abstract::DNA methylation, the most intensively studied epigenetic modification, plays an important role in understanding the molecular basis of diseases. Furthermore, epigenome-wide association study (EWAS) provides a systematic approach to identify epigenetic variants underlying common diseases/phenotypes. However, there is n...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky942
更新日期:2019-01-08 00:00:00
abstract::Computational modelling of mammalian cell cycle regulation is a challenging task, which requires comprehensive knowledge on many interrelated processes in the cell. We have developed a web-based integrated database on cell cycle regulation in mammals in normal and pathological states (Cyclonet database). It integrates...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl912
更新日期:2007-01-01 00:00:00
abstract::Using peptide arrays and binding to native histone proteins, we show that the ADD domain of Dnmt3a specifically interacts with the H3 histone 1-19 tail. Binding is disrupted by di- and trimethylation of K4, phosphorylation of T3, S10 or T11 and acetylation of K4. We did not observe binding to the H4 1-19 tail. The ADD...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq147
更新日期:2010-07-01 00:00:00
abstract::Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz431
更新日期:2019-07-26 00:00:00
abstract::DNA triplet repeats, 5'-d(CTG)n and 5'-d(CAG)n, are present in genes which have been implicated in several neurodegenerative disorders. To investigate possible stable structures formed by these repeating sequences, we have examined d(CTG)n, d(CAG)n and d(CTG).d(CAG)n (n = 2 and 3) using NMR and UV optical spectroscopy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.21.4303
更新日期:1995-11-11 00:00:00
abstract::Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previous preparations of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.9.3175
更新日期:1977-09-01 00:00:00
abstract::DNA damage-induced cell cycle checkpoints serve as surveillance mechanisms to maintain genomic stability, and are regulated by ATM/ATR-mediated signaling pathways that are conserved from yeast to humans. Trypanosoma brucei, an early divergent microbial eukaryote, lacks key components of the conventional DNA damage-ind...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz476
更新日期:2019-09-05 00:00:00
abstract::The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.15.2982
更新日期:1994-08-11 00:00:00