Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications.

abstract：:Understanding the evolutionary and genomic mechanisms responsible for turning the soil-derived saprophytic mycobacteria into lethal intracellular pathogens is a critical step towards the development of strategies for the control of mycobacterial diseases. In this context, Mycobacterium indicus pranii (MIP) is of speci...

journal_title：Nucleic acids research

authors： Saini V,Raghuvanshi S,Khurana JP,Ahmed N,Hasnain SE,Tyagi AK,Tyagi AK

更新日期：2012-11-01 00:00:00

abstract：:We describe the structure (3840 bp) of a novel Euglena gracilis chloroplast ribosomal protein operon that encodes the five genes rpl16-rpl14-rpl5-rps8-rpl36. The gene organization resembles the spc and the 3'-end of the S10 ribosomal protein operons of E. coli. The rpl5 is a new chloroplast gene not previously reporte...

journal_title：Nucleic acids research

authors： Christopher DA,Hallick RB

更新日期：1989-10-11 00:00:00

abstract：:The Diels-Alder reaction between diene-modified oligonucleotides and maleimide-derivatized peptides afforded peptide-oligonucleotide conjugates with high purity and yield. Synthesis of the reagents was easily accomplished by on-column derivatization of the corresponding peptides and oligonucleotides. The cycloaddition...

journal_title：Nucleic acids research

authors： Marchán V,Ortega S,Pulido D,Pedroso E,Grandas A

更新日期：2006-02-14 00:00:00

abstract：:To understand the epigenetic regulation of transcriptional response of macrophages during early-stage M. tuberculosis (Mtb) infection, we performed ChIPseq analysis of H3K4 monomethylation (H3K4me1), a marker of poised or active enhancers. De novo H3K4me1 peaks in infected cells were associated with genes implicated i...

journal_title：Nucleic acids research

authors： Bouttier M,Laperriere D,Memari B,Mangiapane J,Fiore A,Mitchell E,Verway M,Behr MA,Sladek R,Barreiro LB,Mader S,White JH

更新日期：2016-12-15 00:00:00

abstract：:A class of heterogeneous nuclear RNA (hnRNA) molecules contain an internal transcribed poly A sequence of close to 25 uninterrupted AMP residues. HnRNA molecules containing this sequence are separable from those containing the large 3' terminal poly A sequence on the basis of their differential affinity for oligo dT c...

journal_title：Nucleic acids research

authors： Venkatesan S,Nakazato H,Kopp DW,Edmonds M

更新日期：1979-03-01 00:00:00

abstract：:Bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitut...

journal_title：Nucleic acids research

authors： Hartmann RK,Vogel DW,Walker RT,Erdmann VA

更新日期：1988-04-25 00:00:00

abstract：:Bile acid biosynthesis is subjected to feedback regulation whereby bile acids down-regulate their own synthesis. The major point of this regulation is at the level of cholesterol 7alpha-hydroxylase (7alpha-hydroxylase), which controls bile acid output from the classic pathway. This regulation is at the level of transc...

journal_title：Nucleic acids research

authors： del Castillo-Olivares A,Gil G

更新日期：2000-09-15 00:00:00

abstract：:We identify a novel activity of the RarA (also MgsA) protein of Escherichia coli, demonstrating that this protein functions at DNA ends to generate flaps. A AAA+ ATPase in the clamp loader clade, RarA protein is part of a highly conserved family of DNA metabolism proteins. We demonstrate that RarA binds to double-stra...

journal_title：Nucleic acids research

authors： Stanage TH,Page AN,Cox MM

更新日期：2017-03-17 00:00:00

abstract：:The cells of the bronchial epithelium of man are targets for benzo(a)pyrene carcinogenesis. When cultures of these cells, and of non-target fibroblasts, are exposed to [3H]-benzo(a)pyrene, we find that the epithelial cells metabolise and bind to DNA far greater amounts of benzpyrene than do fibroblasts. By analysis of...

journal_title：Nucleic acids research

authors： Arrand JE,Murray AM

更新日期：1982-03-11 00:00:00

abstract：:The human Microprocessor complex cleaves primary microRNA (miRNA) transcripts (pri-miRNAs) to initiate miRNA synthesis. Microprocessor consists of DROSHA (an RNase III enzyme), and DGCR8. DROSHA contains two RNase III domains, RIIIDa and RIIIDb, which simultaneously cleave the 3p- and 5p-strands of pri-miRNAs, respect...

journal_title：Nucleic acids research

authors： Nguyen TL,Nguyen TD,Bao S,Li S,Nguyen TA

更新日期：2020-03-18 00:00:00

abstract：:pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The nucleotide sequence of pS194 has been determined and consists of 4397 base pairs including four open reading frames poten...

journal_title：Nucleic acids research

authors： Projan SJ,Moghazeh S,Novick RP

更新日期：1988-03-25 00:00:00

abstract：:High-throughput sequencing technologies enable direct approaches to catalog and analyze snapshots of the total small RNA content of living cells. Characterization of high-throughput sequencing data requires bioinformatic tools offering a wide perspective of the small RNA transcriptome. Here we present SeqBuster, a hig...

journal_title：Nucleic acids research

authors： Pantano L,Estivill X,Martí E

更新日期：2010-03-01 00:00:00

abstract：:Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of MAR-containing vec...

journal_title：Nucleic acids research

authors： Grandjean M,Girod PA,Calabrese D,Kostyrko K,Wicht M,Yerly F,Mazza C,Beckmann JS,Martinet D,Mermod N

更新日期：2011-08-01 00:00:00

abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...

journal_title：Nucleic acids research

authors： Ohta S,Maruyama S,Nitta K,Sugai S

更新日期：1983-05-25 00:00:00

abstract：:Extracts of the human glioma cell line A1235 (lacking O(6)-methylguanine-DNA methyltransferase) are known to restore a G:T mismatch to a normal G:C pair in a G:T-containing model (45 bp) DNA substrate. Herein we demonstrate that substitution of G:T with O(6)-methylguanine:T (m6G:T) results in extract-induced intra-str...

journal_title：Nucleic acids research

authors： Lari SU,Day RS,Dobler K,Paterson MC

更新日期：2001-06-01 00:00:00

abstract：:Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interac...

journal_title：Nucleic acids research

authors： Palm T,Hemmer K,Winter J,Fricke IB,Tarbashevich K,Sadeghi Shakib F,Rudolph IM,Hillje AL,De Luca P,Bahnassawy L,Madel R,Viel T,De Siervi A,Jacobs AH,Diederichs S,Schwamborn JC

更新日期：2013-04-01 00:00:00

abstract：:PSF (a.k.a. SFPQ) is a ubiquitously expressed, essential nuclear protein with important roles in DNA damage repair and RNA biogenesis. In stimulated T cells, PSF binds to and suppresses the inclusion of CD45 exon 4 in the final mRNA; however, in resting cells, TRAP150 binds PSF and prevents access to the CD45 RNA, tho...

journal_title：Nucleic acids research

authors： Yarosh CA,Tapescu I,Thompson MG,Qiu J,Mallory MJ,Fu XD,Lynch KW

更新日期：2015-10-15 00:00:00

abstract：:OrthoDB is a comprehensive catalog of orthologs, genes inherited by extant species from a single gene in their last common ancestor. In 2016 OrthoDB reached its 9th release, growing to over 22 million genes from over 5000 species, now adding plants, archaea and viruses. In this update we focused on usability of this f...

journal_title：Nucleic acids research

authors： Zdobnov EM,Tegenfeldt F,Kuznetsov D,Waterhouse RM,Simão FA,Ioannidis P,Seppey M,Loetscher A,Kriventseva EV

更新日期：2017-01-04 00:00:00

abstract：:Interferons (IFNs) are a family of multifunctional proteins involved in immune activation, regulation of cell growth and antiviral response. They exert their functions by induction of several IFN-stimulated genes, including IFN regulatory factors (IRFs), a family of transcriptional regulators. One of these factors, IR...

journal_title：Nucleic acids research

authors： Koenig Merediz SA,Schmidt M,Hoppe GJ,Alfken J,Meraro D,Levi BZ,Neubauer A,Wittig B

更新日期：2000-11-01 00:00:00

abstract：:A database (http://perso.curie.fr/tsoussi ) is described, in which over 1000 mutations in the human APC gene of tumors (colon cancer predominantly) are compiled from the literature. It includes both molecular information about the mutations and clinical data about the patients. Software has been designed to analyse al...

journal_title：Nucleic acids research

authors： Laurent-Puig P,Béroud C,Soussi T

更新日期：1998-01-01 00:00:00

abstract：:The Chinese hamster cell line, DC-3F, is heterozygous at the DHFR locus, and each allele can be distinguished on the basis of a unique DNA restriction pattern, protein isoelectric profile and in the abundancy of the DHFR mRNAs it expresses. Although each allele produces four transcripts, 1000, 1650 and 2150 nucleotide...

journal_title：Nucleic acids research

authors： Scotto KW,Yang H,Davide JP,Melera PW

更新日期：1992-12-25 00:00:00

abstract：:The splicing factor Sam68 is upregulated in many human cancers, including prostate cancer (PCa) where it promotes cell proliferation and survival. Nevertheless, in spite of its frequent upregulation in cancer, the mechanism(s) underlying its expression are largely unknown. Herein, bioinformatics analyses identified th...

journal_title：Nucleic acids research

authors： Caggiano C,Pieraccioli M,Panzeri V,Sette C,Bielli P

更新日期：2019-07-09 00:00:00

abstract：:The direct sequencing of DNA generated by the polynucleotide chain reaction, via the incorporation of phosphorothioate nucleotides and followed by treatment with an alkylating reagent that cleaves specifically at the phosphorothioate positions, is described. The Taq polymerase used in the amplification reaction incorp...

journal_title：Nucleic acids research

authors： Nakamaye KL,Gish G,Eckstein F,Vosberg HP

更新日期：1988-11-11 00:00:00

abstract：:Recent studies with poly(ADP-ribose) polymerase (PARP)-deficient mice have highlighted the role of this enzyme in genomic stability and response to various genomic insults. In the absence of DNA damaging treatment, we report here that a PARP-deficient cell line (PARP-/-) established from knockout mice displays a decre...

journal_title：Nucleic acids research

authors： Canitrot Y,de Murcia G,Salles B

更新日期：1998-11-15 00:00:00

abstract：:Sae2 cooperates with the Mre11-Rad50-Xrs2 (MRX) complex to initiate resection of DNA double-strand breaks (DSBs) and to maintain the DSB ends in close proximity to allow their repair. How these diverse MRX-Sae2 functions contribute to DNA damage resistance is not known. Here, we describe mre11 alleles that suppress th...

journal_title：Nucleic acids research

authors： Cassani C,Gobbini E,Vertemara J,Wang W,Marsella A,Sung P,Tisi R,Zampella G,Longhese MP

更新日期：2018-04-06 00:00:00

abstract：:Metnase is a human protein with methylase (SET) and nuclease domains that is widely expressed, especially in proliferating tissues. Metnase promotes non-homologous end-joining (NHEJ), and knockdown causes mild hypersensitivity to ionizing radiation. Metnase also promotes plasmid and viral DNA integration, and topoisom...

journal_title：Nucleic acids research

authors： De Haro LP,Wray J,Williamson EA,Durant ST,Corwin L,Gentry AC,Osheroff N,Lee SH,Hromas R,Nickoloff JA

更新日期：2010-09-01 00:00:00

abstract：:The 0.048502 megabase (Mb), primarily double-stranded DNA of bacteriophage lambda has single-stranded, complementary termini (cohesive ends) that undergo either spontaneous intramolecular joining to form open circular DNA or spontaneous intermolecular joining to form linear, end-to-end oligomeric DNAs (concatemers); c...

journal_title：Nucleic acids research

authors： Louie D,Serwer P

更新日期：1991-06-11 00:00:00

abstract：:Here we analyze the effect of DNA folding on the performance of short primers and describe a simple technique for assessing hitherto uncertain values of thermodynamic parameters that determine the folding of single-stranded DNA into secondary structure. An 8mer with two degenerate positions is extended simultaneously ...

journal_title：Nucleic acids research

authors： Lvovsky L,Ioshikhes I,Raja MC,Zevin-Sonkin D,Sobolev IA,Liberzon A,Shwartzburd J,Ulanovsky LE

更新日期：1998-12-01 00:00:00

abstract：:Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but ...

journal_title：Nucleic acids research

authors： Sen GC,Ghosh HP

更新日期：1976-03-01 00:00:00

abstract：:The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component ...

journal_title：Nucleic acids research

authors： Cribbs DL,Gillam IC,Tener GM

更新日期：1982-10-25 00:00:00