Role of LrpC from Bacillus subtilis in DNA transactions during DNA repair and recombination.

abstract：:The potent anticancer drug actinomycin D (ActD) functions by intercalating into DNA at GpC sites, thereby interrupting essential biological processes including replication and transcription. Certain neurological diseases are correlated with the expansion of (CGG)n trinucleotide sequences, which contain many contiguous...

journal_title：Nucleic acids research

authors： Lo YS,Tseng WH,Chuang CY,Hou MH

更新日期：2013-04-01 00:00:00

abstract：:The ability to induce gene expression in a small molecule dependent manner has led to many applications in target discovery, functional elucidation and bio-production. To date these applications have relied on a limited set of protein-based control mechanisms operating at the level of transcription initiation. The dis...

journal_title：Nucleic acids research

authors： Morra R,Shankar J,Robinson CJ,Halliwell S,Butler L,Upton M,Hay S,Micklefield J,Dixon N

更新日期：2016-02-18 00:00:00

abstract：:In this paper, we report the cloning and sequencing of the C. elegans histidyl-tRNA synthetase gene. The complete genomic sequence, and most of the cDNA sequence, of this gene is now determined. The gene size including flanking and coding regions is 2230 nucleotides long. Three small introns (45-50 bp long) are found ...

journal_title：Nucleic acids research

authors： Amaar YG,Baillie DL

更新日期：1993-09-11 00:00:00

abstract：:Chelation of divalent cations by 5 mM EDTA and subsequent removal by dialysis from a cytoplasmic HeLa cell extract leads to a complete loss of 5S rRNA transcription without affecting tRNA synthesis. Transcription complexes for 5S RNA can no longer be assembled in such a zinc-depleted extract and this ability can be fu...

journal_title：Nucleic acids research

authors： Wingender E,Dilloo D,Seifart KH

更新日期：1984-12-11 00:00:00

abstract：:The genome of the cell is often exposed to DNA damaging agents and therefore requires an intricate well-regulated DNA damage response (DDR) to overcome its deleterious effects. The DDR needs proper regulation for its timely activation, repression, as well as appropriate choice of repair pathway. Studies in Saccharomyc...

journal_title：Nucleic acids research

authors： Jalal D,Chalissery J,Hassan AH

更新日期：2017-03-17 00:00:00

abstract：:A cDNA (2855 nt) encoding a putative cytotoxic ribonuclease (rapLR1) related to the antitumor protein onconase was cloned from a library derived from the liver of gravid female amphibian Rana pipiens. The cDNA was mainly comprised (83%) of 3' untranslated region (UTR). Secondary structure analysis predicted two unusua...

journal_title：Nucleic acids research

authors： Chen S,Le SY,Newton DL,Maizel JV Jr,Rybak SM

更新日期：2000-06-15 00:00:00

abstract：:Human cells are, in general, poor recipients of foreign DNA, which has severely hampered the cloning of genes by direct phenotypic correction of deficient human cell lines after DNA mediated gene transfer. In this communication a methodology is presented which largely circumvents this problems. The method relies on th...

journal_title：Nucleic acids research

authors： Belt PB,Jongmans W,de Wit J,Hoeijmakers JH,van de Putte P,Backendorf C

更新日期：1991-09-25 00:00:00

abstract：:We have determined the genomic structure of the fosB gene and shown that it consists of 4 exons and 3 introns at positions also found in the c-fos gene. By deletion analysis we have characterized a region upstream of the TATA box which is the promoter region of the gene. Several consensus sequences have been identifie...

journal_title：Nucleic acids research

authors： Lazo PS,Dorfman K,Noguchi T,Mattéi MG,Bravo R

更新日期：1992-01-25 00:00:00

abstract：:PipeAlign is a protein family analysis tool integrating a five step process ranging from the search for sequence homologues in protein and 3D structure databases to the definition of the hierarchical relationships within and between subfamilies. The complete, automatic pipeline takes a single sequence or a set of sequ...

journal_title：Nucleic acids research

authors： Plewniak F,Bianchetti L,Brelivet Y,Carles A,Chalmel F,Lecompte O,Mochel T,Moulinier L,Muller A,Muller J,Prigent V,Ripp R,Thierry JC,Thompson JD,Wicker N,Poch O

更新日期：2003-07-01 00:00:00

abstract：:We have identified and sequenced two members of a chicken middle repetitive DNA sequence family. By reassociation kinetics, members of this family (termed CRl) are estimated to be present in 1500-7000 copies per chicken haploid genome. The first family member sequenced (CRlUla) is located approximately 2 kb upstream f...

journal_title：Nucleic acids research

authors： Stumph WE,Kristo P,Tsai MJ,O'Malley BW

更新日期：1981-10-24 00:00:00

abstract：:We present MultiGO, a web-enabled tool for the identification of biologically relevant gene sets from hierarchically clustered gene expression trees (http://ekhidna.biocenter.helsinki.fi/poxo/multigo). High-throughput gene expression measuring techniques, such as microarrays, are nowadays often used to monitor the exp...

journal_title：Nucleic acids research

authors： Kankainen M,Brader G,Törönen P,Palva ET,Holm L

更新日期：2006-01-01 00:00:00

abstract：:Transfection of a human estrogen receptor cDNA expression vector (HEO) into cultured Xenopus kidney cells confers estrogen responsiveness to the recipient cells as demonstrated by the hormone dependent expression of co-transfected Xenopus vitellogenin-CAT chimeric genes. The estrogen stimulation of these vit-CAT genes...

journal_title：Nucleic acids research

authors： Seiler-Tuyns A,Mérillat AM,Haefliger DN,Wahli W

更新日期：1988-09-12 00:00:00

abstract：:The isolation and characterization of deletion mutants of the bacteriocinogenic plasmid Clo DF13 is described. To construct these deletion mutants, DNA of Clo DF13::Tn901 and Clo DF13-rep3::Tn901 plasmids was digested with restriction endonucleases, ligated with T4 ligase and introduced by transformation into Escheric...

journal_title：Nucleic acids research

authors： Stuitje AR,Veltkamp E,van den Elzen PJ,Nijkamp HJ

更新日期：1978-06-01 00:00:00

abstract：:A novel expression system was established that allows expression and propagation of the human hepatitis B virus (HBV) genome in cultured animal cells. An overlength HBV genome encoding the viral pregenomic RNA was put under transcriptional control of the human metallothionein IIA promoter thereby replacing the endogen...

journal_title：Nucleic acids research

authors： Junker M,Galle P,Schaller H

更新日期：1987-12-23 00:00:00

abstract：:Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...

journal_title：Nucleic acids research

authors： Harkins KM,Schaefer NK,Troll CJ,Rao V,Kapp J,Naughton C,Shapiro B,Green RE

更新日期：2020-05-07 00:00:00

abstract：:DnaD and DnaB are essential DNA-replication-initiation proteins in low-G+C content Gram-positive bacteria. Here we use sensitive Hidden Markov Model-based techniques to show that the DnaB and DnaD proteins share a common structure that is evident across all their structural domains, termed DDBH1 and DDBH2 (DnaD DnaB H...

journal_title：Nucleic acids research

authors： Marston FY,Grainger WH,Smits WK,Hopcroft NH,Green M,Hounslow AM,Grossman AD,Craven CJ,Soultanas P

更新日期：2010-11-01 00:00:00

abstract：:Immunoglobulin (Ig) diversification by somatic hypermutation in germinal center B cells is instrumental for maturation of the humoral immune response, but also bears the risk of excessive or aberrant genetic changes. Thus, introduction of DNA damage by activation-induced cytidine deaminase as well as DNA repair by mul...

journal_title：Nucleic acids research

authors： Frankenberger S,Davari K,Fischer-Burkart S,Böttcher K,Tomi NS,Zimber-Strobl U,Jungnickel B

更新日期：2014-04-01 00:00:00

abstract：:Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization...

journal_title：Nucleic acids research

authors： Uno N,Kaku N,Morinaga Y,Hasegawa H,Yanagihara K

更新日期：2020-09-04 00:00:00

abstract：:Eukaryotic cellular mRNAs contain a cap at their 5'-ends, but some viral and cellular mRNAs bypass the cap-dependent mechanism of translation initiation in favor of internal entry of ribosomes at specific RNA sequences. Cap-dependent initiation requires intact initiation factor eIF4G (formerly eIF-4gamma, eIF-4Fgamma ...

journal_title：Nucleic acids research

authors： Keiper BD,Rhoads RE

更新日期：1997-01-15 00:00:00

abstract：:The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities. In this system, recombi...

journal_title：Nucleic acids research

authors： Gersbach CA,Gaj T,Gordley RM,Barbas CF 3rd

更新日期：2010-07-01 00:00:00

abstract：:We report the isolation of a novel human POU domain encoding gene named RDC-1. The POU domain of the RDC-1 encoded protein is highly related to the POU domain potentially encoded by the rat brain-3 sequence and to that of the Drosophila I-POU protein; outside of the POU region, RDC-1 is unrelated to any previously cha...

journal_title：Nucleic acids research

authors： Collum RG,Fisher PE,Datta M,Mellis S,Thiele C,Huebner K,Croce CM,Israel MA,Theil T,Moroy T

更新日期：1992-09-25 00:00:00

abstract：:Insertion of the four major nucleotides at the 5'-side of the anticodon triplet of E. coli tRNAMetf was performed by joining of the half molecules obtained by limited digestion with RNase A and the chemically synthesized tetranucleotide pN-C-A-U using RNA ligase. Insertion of U-U at the 5'-side or A and A-A at the 3'-...

journal_title：Nucleic acids research

authors： Doi T,Yamane A,Matsugi J,Ohtsuka E,Ikehara M

更新日期：1985-05-24 00:00:00

abstract：:A sequence of 89 nucleotides from rabbit beta-globin mRNA has been determined and is shown to code for residues 107 to 137 of the beta-globin protein. In addition, a sequence heterogeneity has been identified within this 89 nucleotide long sequence which corresponds to a known polymorphic variant of rabbit beta-globin...

journal_title：Nucleic acids research

authors： Proudfoot NJ

更新日期：1976-07-01 00:00:00

abstract：:The DNA sequence of a 225.4 kilobase segment of the Escherichia coli K-12 genome is described here, from 76.0 to 81.5 minutes on the genetic map. This brings the total of contiguous sequence from the E.coli genome project to 725.1 kb (76.0 to 92.8 minutes). We found 191 putative coding genes (ORFs) of which 72 genes w...

journal_title：Nucleic acids research

authors： Sofia HJ,Burland V,Daniels DL,Plunkett G 3rd,Blattner FR

更新日期：1994-07-11 00:00:00

abstract：:To understand how potential for G-quadruplex formation might influence regulation of gene expression, we examined the 2 kb spanning the transcription start sites (TSS) of the 18 217 human RefSeq genes, distinguishing contributions of template and nontemplate strands. Regions both upstream and downstream of the TSS are...

journal_title：Nucleic acids research

authors： Eddy J,Maizels N

更新日期：2008-03-01 00:00:00

abstract：:We recently developed a new type of pyrrole (Py)-imidazole (Im) polyamide-tetrahydrocyclopropabenzindolone (CBI) conjugate with an indole linker as a stable sequence-specific alkylating agent. In this study, we investigated the gene silencing activities of polyamides A, B and C, which selectively alkylate specific seq...

journal_title：Nucleic acids research

authors： Shinohara K,Sasaki S,Minoshima M,Bando T,Sugiyama H

更新日期：2006-02-25 00:00:00

abstract：:Chromatin fragments released from intact Friend erythroleukemia cell nuclei during limited incubation with micrococcal nuclease, DNase II or DNase I were analyzed to determine the distribution of DNA methyltransferase in chromatin. The enzyme was released in a free form when internucleosomal DNA was digested with micr...

journal_title：Nucleic acids research

authors： Creusot F,Christman JK

更新日期：1981-10-24 00:00:00

abstract：:Re-sequencing, the identification of the specific variants in a sequence of interest compared with a known genomic sequence, is a ubiquitous task in today's biology. Universal arrays, which interrogate all possible oligonucleotides of a certain length in a target sequence, have been suggested for computationally deter...

journal_title：Nucleic acids research

authors： Pe'er I,Arbili N,Liu Y,Enck C,Gelfand CA,Shamir R

更新日期：2003-10-01 00:00:00

abstract：:The Pk-rec gene, encoding a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcussp. KOD1, was expressed in Escherichia coli. The recombinant Pk-REC was purified to homogeneity and was shown to be in a dimeric form. A striking property of the purified recombinant Pk-REC was the unusual DNase activity on ...

journal_title：Nucleic acids research

authors： Rashid N,Morikawa M,Nagahisa K,Kanaya S,Imanaka T

更新日期：1997-02-15 00:00:00

abstract：:Differences between species have been suggested to largely reside in the network of connections among the genes. Nevertheless, the rate at which these connections evolve has not been properly quantified. Here, we measure the extent to which co-regulation between pairs of genes is conserved over large phylogenetic dist...

journal_title：Nucleic acids research

authors： Snel B,van Noort V,Huynen MA

更新日期：2004-09-07 00:00:00