Abstract:
:In this paper, we report the cloning and sequencing of the C. elegans histidyl-tRNA synthetase gene. The complete genomic sequence, and most of the cDNA sequence, of this gene is now determined. The gene size including flanking and coding regions is 2230 nucleotides long. Three small introns (45-50 bp long) are found to interrupt the open reading frame. The open reading frame translates to 523 amino acids. This putative protein sequence shows extensive homology with the human and yeast histidyl-tRNA the histidyl-tRNA synthetase gene is a single copy gene. Hence, it is very likely that it encodes both the cytoplasmic and the mitochondrial histidyl-tRNA synthetases. It is likely to be trans-spliced since it contains a trans-splice site in its 5' untranslated region.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Amaar YG,Baillie DLdoi
10.1093/nar/21.18.4344subject
Has Abstractpub_date
1993-09-11 00:00:00pages
4344-7issue
18eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract::Although secondary structure predictions of an individual RNA sequence have been widely used in a number of sequence analyses of RNAs, accuracy is still limited. Recently, we proposed a method (called 'CentroidHomfold'), which includes information about homologous sequences into the prediction of the secondary structu...
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journal_title:Nucleic acids research
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