Abstract:
:We had shown previously (3) that the E. coli argE gene could be turned-on by an IS3 element inserted in orientation II near the 5' end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the S1 nuclease mapping technique with in vivo transcribed RNA we located the promoter responsible for argE transcription on IS3 itself outside the region involved in the inverted repeats of this element. IS3 may therefore be considered as a mobile promoter.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Charlier D,Piette J,Glansdorff Ndoi
10.1093/nar/10.19.5935subject
Has Abstractpub_date
1982-10-11 00:00:00pages
5935-48issue
19eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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journal_title:Nucleic acids research
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