Abstract：
:The entire nucleotide sequence of the Ad. 2 EcoRI D fragment has been determined using the Maxam and Gilbert method. This sequence of 2678 bp contains informations relative to late mRNAs ending at position 78 and for which an AATAAA sequence corresponding to their 3' ends is found at residue number 833. Position of the PVIII mRNA is determined thus allowing deduction of the probable amino acid sequence of the PVIII protein. The position and the sequence of the first leader of early 3 mRNAs is determined as well as the sequence and position of the second early leader of region 3 mRNAs, which also correspond to the "y" leader of the fiber mRNA. Following the localization of an open reading frame in which an ATG could initiate protein synthesis it can be predicted that 3a, b, c mRNAs code for the 16K early protein and the probable amino acid sequence of this protein can be deduced. The CAGTTT sequence frequently present at the 5' end of a leader or of a mRNA body as well as the GGTGAG sequence which is found at the 3' end of several leaders were used to postulate the position of various early mRNAs of region 3 and to suggest the existence of an additional splicing event during the processing of mRNAs 3a, b and c. They were also used to predict the position of the additional "x" late leaders. The imbrication of information concerning (i) the family of late mRNAs ending at position 78, (ii) the position of the "x" leader and the "y" leader and (iii) the beginning of early region 3 is also depicted.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hérissé J,Courtois G,Galibert Fdoi
10.1093/nar/8.10.2173subject
Has Abstractpub_date
1980-05-24 00:00:00pages
2173-92issue
10eissn
0305-1048issn
1362-4962journal_volume
8pub_type
杂志文章abstract：:The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1178
更新日期：2012-01-01 00:00:00
abstract：:Selenoproteins contain the amino acid selenocysteine which is encoded by a UGA Sec codon. Recoding UGA Sec requires a complex mechanism, comprising the cis-acting SECIS RNA hairpin in the 3'UTR of selenoprotein mRNAs, and trans-acting factors. Among these, the SECIS Binding Protein 2 (SBP2) is central to the mechanism...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp078
更新日期：2009-04-01 00:00:00
abstract：:SLM2 and Sam68 are splicing regulator paralogs that usually overlap in function, yet only SLM2 and not Sam68 controls the Neurexin2 AS4 exon important for brain function. Herein we find that SLM2 and Sam68 similarly bind to Neurexin2 pre-mRNA, both within the mouse cortex and in vitro. Protein domain-swap experiments ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1277
更新日期：2017-04-20 00:00:00
abstract：:Expressed Sequence Tag-based gene expression profiling can be used to discover functionally associated genes on a large scale. Currently available web servers and tools focus on finding differentially expressed genes in different samples or tissues rather than finding co-expressed genes. To fill this gap, we have deve...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki480
更新日期：2005-07-01 00:00:00
abstract：:Replication factor C (RF-C) is a eukaryotic heteropentameric protein required for DNA replication and repair processes by loading proliferating cell nuclear antigen (PCNA) onto DNA in an ATP-dependent manner. Prior to loading PCNA, RF-C binds to DNA. This binding is thought to be restricted to a specific DNA structure...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.17.3433
更新日期：1999-09-01 00:00:00
abstract：:E2F transcription factors (E2F1-8) are known to coordinately regulate the expression of a plethora of target genes, including those coding for microRNAs (miRNAs), to control cell cycle progression. Recent work has described the atypical E2F factor E2F7 as a transcriptional repressor of cell cycle-related protein-codin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw146
更新日期：2016-07-08 00:00:00
abstract：:Single cell RNA-seq experiments provide valuable insight into cellular heterogeneity but suffer from low coverage, 3' bias and technical noise. These unique properties of single cell RNA-seq data make study of alternative splicing difficult, and thus most single cell studies have restricted analysis of transcriptome v...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1525
更新日期：2016-05-05 00:00:00
abstract：:The Bayesian regularization method for high-throughput differential analysis, described in Baldi and Long (A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics 2001: 17: 509-519) and implemented in the Cyber-T web server, is ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks420
更新日期：2012-07-01 00:00:00
abstract：:Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.18.3745
更新日期：1997-09-15 00:00:00
abstract：:TAP/hNXF1 is a key factor that mediates general cellular mRNA export from the nucleus, and its orthologs are structurally and functionally conserved from yeast to humans. Metazoans encode additional proteins that share homology and domain organization with TAP/hNXF1, suggesting their participation in mRNA metabolism; ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki706
更新日期：2005-07-13 00:00:00
abstract：:We have studied the effect of selenium on the expression of a cellular glutathione peroxidase, GSHPx-1, in transfected MCF-7 cells and in doxorubicin-resistant (Adrr) MCF-7 cells. A GSHPx-1 cDNA with a Rous Sarcoma virus promoter was transfected into a human mammary carcinoma cell line, MCF-7, which has very low endog...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.6.1531
更新日期：1990-03-25 00:00:00
abstract：:Cellular RNA labeling strategies based on bioorthogonal chemical reactions are much less developed in comparison to glycan, protein and DNA due to its inherent instability and lack of effective methods to introduce bioorthogonal reactive functionalities (e.g. azide) into RNA. Here we report the development of a simple...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv903
更新日期：2016-01-29 00:00:00
abstract：:The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases ind...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.17.6205
更新日期：1985-09-11 00:00:00
abstract：:Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specifi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.10.2199
更新日期：2001-05-15 00:00:00
abstract：:Three protein isoforms (74, 54 and 42 kDa) are expressed from repressor gene c in the Streptomyces temperate bacteriophage phiC31. Because expression of the two smaller isoforms, 54 and 42 kDa, is sufficient for superinfection immunity, the interaction between these isoforms was studied. The native 42 kDa repressor (N...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.10.2457
更新日期：1998-05-15 00:00:00
abstract：:The presence of a homing endonuclease gene (HEG) within a microbial intron or intein empowers the entire element with the ability to invade genomic targets. The persistence of a homing endonuclease lineage depends in part on conservation of its DNA target site. One such rDNA sequence has been invaded both in archaea a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn846
更新日期：2008-12-01 00:00:00
abstract：:Genome browsers are widely used for individually exploring various types of genomic data. A handful of genome browsers offer limited tools for collaboration among multiple users. Here, we describe PBrowse, an integrated real-time collaborative genome browser that enables multiple users to simultaneously view and acces...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1358
更新日期：2017-05-19 00:00:00
abstract：:Three copies of a highly repetitive DNA sequence B1 which is complementary to the most abundant class of mouse fold-back RNA have been cloned in pBR322 plasmid and sequenced by the method of Maxam and Gilbert. All the three have a length of about 130 base pairs and are very similar in their base sequence. The deviatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.6.1201
更新日期：1980-03-25 00:00:00
abstract：:The University of Minnesota Biocatalysis/Biodegradation Database (http://www.labmed.umn.edu/umbbd/ ) begins its fifth year having met its initial goals. It contains approximately 100 pathways for microbial catabolic metabolism of primarily xenobiotic organic compounds, including information on approximately 650 reacti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.1.377
更新日期：2000-01-01 00:00:00
abstract：:The ProtoNet site provides an automatic hierarchical clustering of the SWISS-PROT protein database. The clustering is based on an all-against-all BLAST similarity search. The similarities' E-score is used to perform a continuous bottom-up clustering process by applying alternative rules for merging clusters. The outco...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg096
更新日期：2003-01-01 00:00:00
abstract：:High-throughput chromosome conformation capture (3C) technologies, such as Hi-C, have made it possible to survey 3D genome structure. However, obtaining 3D profiles at kilobase resolution at low cost remains a major challenge. Therefore, we herein present an algorithm for precise identification of chromatin interactio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx885
更新日期：2017-12-15 00:00:00
abstract：:Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg561
更新日期：2003-07-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) resides in a high ROS environment and suffers more mutations than its nuclear counterpart. Increasing evidence suggests that mtDNA mutations are not the results of direct oxidative damage, rather are caused, at least in part, by DNA replication errors. To understand how the mtDNA replicase, P...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1018
更新日期：2020-01-24 00:00:00
abstract：:Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.11.3969
更新日期：1985-06-11 00:00:00
abstract：:Selenocysteine (Sec), the 21(st) amino acid in translation, uses its specific tRNA (tRNA(Sec)) to recognize the UGA codon. The Sec-specific elongation factor SelB brings the selenocysteinyl-tRNA(Sec) (Sec-tRNA(Sec)) to the ribosome, dependent on both an in-frame UGA and a Sec-insertion sequence (SECIS) in the mRNA. Th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv833
更新日期：2015-10-15 00:00:00
abstract：:We describe the unique features of an aberrantly rearranged mu immunoglobulin heavy chain gene isolated from MPC-11 cells (a gamma 2b producing Balb/c plasmacytoma). A novel rearrangement has occurred 1.5 Kb 5' of the MPC-11 mu gene (denoted 18b mu) resulting in the deletion of the majority of the repetitive switch re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.23.7751
更新日期：1982-12-11 00:00:00
abstract：:Here we describe a method of labelling short oligonucleotide probes with enzyme without purification or chemical modifications. Biotinylated oligonucleotides as short as 10 nt are coupled with streptavidin-conjugated enzyme, hybridised and detected with enzyme-triggered chemiluminescence. The detection of hybridisatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.2.703
更新日期：1999-01-15 00:00:00
abstract：:Proteomic and RNomic approaches have identified many components of different ribonucleoprotein particles (RNPs), yet still little is known about the organization and protein proximities within these heterogeneous and highly dynamic complexes. Here we describe a targeted cross-linking approach, which combines cross-lin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1366
更新日期：2016-02-18 00:00:00
abstract：:Unlike most yeast ribosomal protein genes studied so far the genes coding for S24 and L46 are adjacent on the genome. Sequence analysis showed that the two genes are transcribed divergently, their initiation codons being 630 bp apart. Taking the respective ATG translation start sites as reference points, the 5'- end o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.3.701
更新日期：1985-02-11 00:00:00
abstract：:RegulonDB is a DataBase that integrates biological knowledge of the mechanisms that regulate the transcription initiation in Escherichia coli , as well as knowledge on the organization of the genes and regulatory signals into operons in the chromosome. The operon is the basic structure used in RegulonDB to describe th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.55
更新日期：1998-01-01 00:00:00
