Abstract：
:Drosophila tissue culture cells have been important in the study of homologous promoters and more recently in the study of mammalian transcriptional factors such as CTF and SP1 which bind and stimulate transcription from transfected genes. In this paper we show that a Drosophila melanogaster haploid cell line (1182-4), not previously used for transfection studies, is capable of taking up and expressing DNA without the use of a facilitating agent such as calcium phosphate. Furthermore expression from a variety of Drosophila promoters such as copia, heatshock and rudimentary as well as a mammalian promoter RSV-LTR, show between 20 and over 100 times more activity in 1182-4 cells than in D.hydei DH33 or D.melanogaster S3, or D1 cell lines. This cell line should prove to be particularly useful for the analysis of weak promoters and heterologous transcription factors.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Saunders SE,Rawls JM,Wardle CJ,Burke JFdoi
10.1093/nar/17.15.6205subject
Has Abstractpub_date
1989-08-11 00:00:00pages
6205-16issue
15eissn
0305-1048issn
1362-4962journal_volume
17pub_type
杂志文章abstract：:We have isolated and characterized DNA probes that detect homologies between the X and Y chromosomes. Clone St25 is derived from the q13-q22 region of the X chromosome and recognizes a 98% homologous sequence on the Y chromosome. Y specific fragments were present in DNAs from 5 Yq-individuals and from 4 out of 7 XX ma...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.15.5485
更新日期：1985-08-12 00:00:00
abstract：:NMR data from different experiments often contain errors; thus, automated backbone resonance assignment is a very challenging issue. In this paper, we present a method called GANA that uses a genetic algorithm to automatically perform backbone resonance assignment with a high degree of precision and recall. Precision ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki768
更新日期：2005-08-10 00:00:00
abstract：:Assembly factors provide speed and directionality to the maturation process of the 30S subunit in bacteria. To gain a more precise understanding of how these proteins mediate 30S maturation, it is important to expand on studies of 30S assembly intermediates purified from bacterial strains lacking particular maturation...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz571
更新日期：2019-09-05 00:00:00
abstract：:The alcohol dehydrogenase (Adh) gene of D. melanogaster is transcribed from two different promoters during fly development: the distal (adult) and the proximal (embryonic-larval). Certain aspects of Adh gene regulation are represented in Drosophila continuous cell lines. We have used Drosophila tissue culture cells in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.19.7903
更新日期：1987-10-12 00:00:00
abstract：:The expression of the nicotinic acetylcholine receptor alpha 2 subunit gene is highly restricted to the Spiriform lateralis nucleus of the Chick diencephalon. As a first step toward understanding the molecular mechanism underlying this regulation, we have investigated the structural and regulatory properties of the 5'...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.9.2185
更新日期：1993-05-11 00:00:00
abstract：:The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.11.4481
更新日期：1987-06-11 00:00:00
abstract：:Two DNA polymerases have been purified from the 105,000 x g supernatant of ungerminated wheat. The purification stages included: high speed centrifugation, salt fractionation, DEAE-cellulose chromatography, Sephadex G-150 filtration and phosphocellulose chromatography. Several properties of the two enzyme (called A an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.11.2077
更新日期：1975-11-01 00:00:00
abstract：:Hepatitis C virus (HCV) is the major etiological agent of non-A, non-B hepatitis. Current therapies are not effective in all patients and can result in the generation of resistant mutants, leading to a need for new therapeutic options. HCV has an RNA genome that contains a well-defined and highly conserved secondary s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh706
更新日期：2004-07-19 00:00:00
abstract：:Transcription is the first step in gene expression, and it is the step at which most of the regulation of expression occurs. Although sequenced prokaryotic genomes provide a wealth of information, transcriptional regulatory networks are still poorly understood using the available genomic information, largely because a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr795
更新日期：2012-02-01 00:00:00
abstract：:Nucleic acids possess the unique property of being enzymatically amplifiable, and have therefore been a popular choice for the combinatorial selection of functional sequences, such as aptamers or ribozymes. However, amplification typically requires known sequence segments that serve as primer binding sites, which can ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks004
更新日期：2012-05-01 00:00:00
abstract：:Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2 splicing in respon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv223
更新日期：2015-04-30 00:00:00
abstract：:Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn918
更新日期：2009-02-01 00:00:00
abstract：:PrankWeb is an online resource providing an interface to P2Rank, a state-of-the-art method for ligand binding site prediction. P2Rank is a template-free machine learning method based on the prediction of local chemical neighborhood ligandability centered on points placed on a solvent-accessible protein surface. Points...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz424
更新日期：2019-07-02 00:00:00
abstract：:Numerous potential ligand-binding sites are available today, along with hundreds of thousands of known binding sites observed in the PDB. Exhaustive similarity search for such vastly numerous binding site pairs is useful to predict protein functions and to enable rapid screening of target proteins for drug design. Exi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1130
更新日期：2012-01-01 00:00:00
abstract：:Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional charact...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq119
更新日期：2010-05-01 00:00:00
abstract：:Maintenance of stem-cell identity requires proper regulation of enhancer activity. Both transcription factors OCT4/SOX2/NANOG and histone methyltransferase complexes MLL/SET1 were shown to regulate enhancer activity, but how they are regulated in embryonic stem cells (ESCs) remains further studies. Here, we report a t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkab034
更新日期：2021-01-27 00:00:00
abstract：:Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku955
更新日期：2015-01-01 00:00:00
abstract：:chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression profiling. The server implements a probe selection algorithm that takes as an input, in addition to the target sequences...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq517
更新日期：2010-07-01 00:00:00
abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx500
更新日期：2017-08-21 00:00:00
abstract：:Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz860
更新日期：2020-01-08 00:00:00
abstract：:The 80S ribosome from Saccharomyces cerevisiae has been reconstructed from cryo electron micrographs to a resolution of 35 A. It is strikingly similar to the 70S ribosome from Escherichia coli, while displaying the characteristic eukaryotic features familiar from reconstructions of ribosomes from higher eukaryotes. As...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.2.655
更新日期：1998-01-15 00:00:00
abstract：:The dominant kanr marker gene plays an important role in gene disruption experiments in budding yeast, as this marker can be used in a variety of yeast strains lacking the conventional yeast markers. We have developed a loxP-kanMX-loxP gene disruption cassette, which combines the advantages of the heterologous kanr ma...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.13.2519
更新日期：1996-07-01 00:00:00
abstract：:Triethylammonium uridine-3',5'-cyclic phosphorothioate crystallizes in space group P2(1)2(1)2(1), a = 7.177(1), b = 13.155(6), c = 21.114(7) A, C15H26N3O7PS, MW 423.4, Z = 4, dx = 1.41g/cm3. The crystal structure was solved by direct methods on the basis of 1493 counter X-ray diffraction data (CuK alpha) and refined t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.12.4945
更新日期：1987-06-25 00:00:00
abstract：:The GWIPS-viz browser (http://gwips.ucc.ie/) is an on-line genome browser which is tailored for exploring ribosome profiling (Ribo-seq) data. Since its publication in 2014, GWIPS-viz provides Ribo-seq data for an additional 14 genomes bringing the current total to 23. The integration of new Ribo-seq data has been auto...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx790
更新日期：2018-01-04 00:00:00
abstract：:Clustered damages are formed in DNA by ionising radiation and radiomimetic anticancer agents and are thought to be biologically severe. 7,8-dihydro-8-oxoguanine (8-oxoG), a major DNA damage resulting from oxidative attack, is highly mutagenic leading to a high level of G.C-->T.A transversions if not previously excised...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.5.1107
更新日期：2001-03-01 00:00:00
abstract：:Recent data showed that p53 stimulates the expression of genes encoding not only pro- but also antioxidant enzymes. It was suggested that antioxidant genes could be induced under physiologic levels of stress while the prooxidant ones respond to higher level of stress. Results presented in this article illustrate an ad...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm824
更新日期：2007-01-01 00:00:00
abstract：:We present evidence of formation of an intramolecular parallel triple helix with T*A.T and G*G.C base triplets (where * represents the hydrogen bonding interaction between the third strand and the duplex while. represents the Watson-Crick interactions which stabilize the duplex). The third GT strand, containing seven ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.18.3511
更新日期：2000-09-15 00:00:00
abstract：:RaftProt (http://lipid-raft-database.di.uq.edu.au/) is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1131
更新日期：2015-01-01 00:00:00
abstract：:RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku422
更新日期：2014-07-01 00:00:00
abstract：:A DNA methylase has been purified 270-fold from HeLa cell nuclei by chromatography on DEAE-cellulose, phosphocellulose, and hydroxyapatite. The enzyme transfers methyl groups from S-adenosyl-L-methionine to cytosine residues in DNA. The sole product of the reaction has been identified as 5-methylcytosine. The enzyme i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/2.10.1669
更新日期：1975-10-01 00:00:00
