Abstract:
:Mutant AraC proteins were selected for their ability to induce but not to repress, or their ability to repress but not to induce the araBAD operon. One such unusual mutant is able to bind to the araI site with an affinity only two to three-fold weaker than the wild type AraC protein, but the mutant protein was shown, both in crude extracts and when purified, to contact only two of the three major groove regions of the DNA that are contacted by the wild type protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brunelle A,Hendrickson W,Schleif Rdoi
10.1093/nar/13.14.5019subject
Has Abstractpub_date
1985-07-25 00:00:00pages
5019-26issue
14eissn
0305-1048issn
1362-4962journal_volume
13pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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