Abstract:
:A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs by gel filtration chromatography in the presence of a high concentration of salt. The complementary RNAs are released from the R-loops by heating, and are assayed by gel electrophoresis or cell free translation to determine their purity and to identify the proteins for which they code. We have demonstrated that recombinant DNAs containing sequences for abundant or moderately abundant mRNAs of Saccharomyces cerevisiae can be identified by this means.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Woolford JL Jr,Rosbash Mdoi
10.1093/nar/6.7.2483subject
Has Abstractpub_date
1979-06-11 00:00:00pages
2483-97issue
7eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract::The open state of human topoisomerase I has been probed by molecular dynamics simulation, starting from the coordinates of the closed structure of the protein complexed with DNA, after elimination of the 22-bp DNA duplex oligonucleotide. A repulsion force between the two lips of the protein has been introduced for a s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm199
更新日期:2007-01-01 00:00:00
abstract::A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two promi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.12.4969
更新日期:1986-06-25 00:00:00
abstract::PPARγ2 is a critical lineage-determining transcription factor that is essential for adipogenic differentiation. Here we report characterization of the three-dimensional structure of the PPARγ2 locus after the onset of adipogenic differentiation and the mechanisms by which it forms. We identified a differentiation-depe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw129
更新日期:2016-06-20 00:00:00
abstract::The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.20.6383
更新日期:1982-10-25 00:00:00
abstract::Alternative splicing has recently emerged as a major mechanism of regulation in the human genome, occurring in perhaps 40-60% of human genes. Thus, microarray studies of functional regulation could, in principle, be extended to detect not only the changes in the overall expression of a gene, but also changes in its sp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh173
更新日期:2004-12-14 00:00:00
abstract::Plants have large diverse families of small secreted proteins (SSPs) that play critical roles in the processes of development, differentiation, defense, flowering, stress response, symbiosis, etc. Oryza sativa is one of the major crops worldwide and an excellent model for monocotyledonous plants. However, there had no...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1090
更新日期:2013-01-01 00:00:00
abstract::Sug1 and Sug2 are two of six ATPases in the 19S regulatory particle of the 26S proteasome. We have shown previously that these proteins play a non-proteolytic role in the transcription of the GAL genes in yeast. In this study, we probe the requirement for these factors in stress-induced transcription in yeast. It is k...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl012
更新日期:2006-03-03 00:00:00
abstract::The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein id...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.1.131
更新日期:1985-01-11 00:00:00
abstract::YY1 is ubiquitously expressed zinc finger DNA binding protein. It can act as a transcriptional repressor or activator and, when binding at the initiator element, as a component of the basal transcription complex. Binding sites for YY1 have been reported in a wide variety of promoters and they exhibit substantial diver...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.21.4457
更新日期:1995-11-11 00:00:00
abstract::TFIIH is a multisubunit factor essential for transcription initiation and promoter escape of RNA polymerase II and for the opening of damaged DNA double strands in nucleotide excision repair (NER). In this study, we have analyzed at which step of the transcription cycle TFIIH is essential for transcription by RNA poly...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr746
更新日期:2012-01-01 00:00:00
abstract::Circular DNA can arise from all parts of eukaryotic chromosomes. In yeast, circular ribosomal DNA (rDNA) accumulates dramatically as cells age, however little is known about the accumulation of other chromosome-derived circles or the contribution of such circles to genetic variation in aged cells. We profiled circular...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa545
更新日期:2020-08-20 00:00:00
abstract::This paper describes a simple program that uses a digitizing device to enter DNA sequences directly from autoradiographs into a computer. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part2.499
更新日期:1984-01-11 00:00:00
abstract::Escherichia coli Exonuclease I (ExoI) digests single-stranded DNA (ssDNA) in the 3'-5' direction in a highly processive manner. The crystal structure of ExoI, determined previously in the absence of DNA, revealed a C-shaped molecule with three domains that form a central positively charged groove. The active site is a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt278
更新日期:2013-06-01 00:00:00
abstract::A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.7.2493
更新日期:1978-07-01 00:00:00
abstract::Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome and minimize genomic instability. We have investigated how the cleav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku172
更新日期:2014-05-01 00:00:00
abstract::Here we report that the poor binding of methylphosphonate oligodeoxynucleosides (MP-ODNs) to their nucleic acid targets can be improved by additional inversion of the anomeric configuration (from beta to alpha) in the sugar moieties to give a new class of analogs, MP alpha-oligonucleosides. MP alpha-dT12and MP 5' alph...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.20.4551
更新日期:1998-10-15 00:00:00
abstract::The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.7.2213
更新日期:1977-07-01 00:00:00
abstract::Interest in secondary metabolites such as RiPPs (ribosomally synthesized and posttranslationally modified peptides) is increasing worldwide. To facilitate the research in this field we have updated our mining web server. BAGEL4 is faster than its predecessor and is now fully independent from ORF-calling. Gene clusters...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky383
更新日期:2018-07-02 00:00:00
abstract::Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced sile...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw776
更新日期:2016-11-02 00:00:00
abstract::The mitochondrial DNA (mtDNA) control regions for common chimpanzee, pygmy chimpanzee and gorilla were sequenced and the lengths and termini of their D-loop DNA's characterized. In these and all other species for which there are data, 5' termini map to sequences that contain the trinucleotide YAY. 3' termini are 25-51...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.13.5841
更新日期:1988-07-11 00:00:00
abstract::MiRNAs are important regulators of gene expression and are frequently deregulated under pathologic conditions. They are highly stable in bodily fluids which makes them feasible candidates to become minimally invasive biomarkers. In fact, several studies already proposed circulating miRNA-based biomarkers for different...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky981
更新日期:2019-01-08 00:00:00
abstract::The expression of Herpes Simplex Virus 1 (HSV-1) glycoprotein C (gC), a well defined herpesvirus late gene, was studied by linking the promoter-regulatory region of this gene to the coding sequences for the bacterial enzyme, beta-galactosidase (beta-gal). A chimeric gene, containing the beta-gal gene under the control...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.21.10267
更新日期:1988-11-11 00:00:00
abstract::Human thymine-DNA glycosylase (TDG) is well known to excise thymine and uracil from G.T and G.U mismatches, respectively, and was therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine. In this study, we characterized ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg344
更新日期:2003-05-01 00:00:00
abstract::The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.ed...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki038
更新日期:2005-01-01 00:00:00
abstract::Organisms growing at elevated temperatures face the challenge of maintaining the integrity of their genetic materials. Archaea possess unique chromatin proteins for gene organization and information processing. We present the solution structure of Sso7c4 from Sulfolobus solfataricus, which has a homodimeric DNA-bindin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr283
更新日期:2011-08-01 00:00:00
abstract::Targeted gene silencing by RNA interference allows the study of gene function in plants and animals. In cell culture and small animal models, genetic screens can be performed--even tissue-specifically in Drosophila--with genome-wide RNAi libraries. However, a major problem with the use of RNAi approaches is the unavoi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp450
更新日期:2009-07-01 00:00:00
abstract::In this paper, we report investigations, based on circular dichroism, nuclear magnetic resonance spectroscopy and electrophoresis methods, on three oligonucleotide sequences, each containing one 3'-3' and two 5'-5' inversion of polarity sites, and four G-runs with a variable number of residues, namely two, three and f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx566
更新日期:2017-08-21 00:00:00
abstract::In this paper, we describe a new method for selecting RNA aptamers that cooperatively bind to two specific sites within a target RNA. We designed a selection system in which two RNAs, a target RNA and a RNA pool, were assembled by employing a pre-organized GAAA tetraloop-11-nt receptor interaction. This allows us to s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn1012
更新日期:2009-02-01 00:00:00
abstract::We describe here an energy based computer software suite for narrowing down the search space of tertiary structures of small globular proteins. The protocol comprises eight different computational modules that form an automated pipeline. It combines physics based potentials with biophysical filters to arrive at 10 pla...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl789
更新日期:2006-01-01 00:00:00
abstract::In eukaryotes, RNA-binding proteins that contain multiple K homology (KH) domains play a key role in coordinating the different steps of RNA synthesis, metabolism and localization. Understanding how the different KH modules participate in the recognition of the RNA targets is necessary to dissect the way these protein...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks368
更新日期:2012-08-01 00:00:00