Abstract:
:The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiation site). The promoter was cleaved into two fragments by R-Hha I and each promoter fragment was joined to DNA fragments derived from other regions. Ligation of the TATAAT-containing fragment to any of the DNA fragments examined resulted in recovery of promoter function. The results suggest for this type of promoter that no unique sequence is necessary upstream from the R-Hha I cleavage site although a contiguous DNA chain must be present in this area.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Okamoto T,Sugimoto K,Sugisaki H,Takanami Mdoi
10.1093/nar/4.7.2213subject
Has Abstractpub_date
1977-07-01 00:00:00pages
2213-22issue
7eissn
0305-1048issn
1362-4962journal_volume
4pub_type
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