Nucleotide sequence, genomic organization and evolution of a major repetitive DNA family in tilapia (Oreochromis mossambicus/hornorum).

abstract：:Enhancer-dependent transcription involving the promoter specificity factor σ(54) is widely distributed amongst bacteria and commonly associated with cell envelope function. For transcription initiation, σ(54)-RNA polymerase yields open promoter complexes through its remodelling by cognate AAA+ ATPase activators. Since...

journal_title：Nucleic acids research

authors： Schaefer J,Engl C,Zhang N,Lawton E,Buck M

更新日期：2015-09-03 00:00:00

abstract：:Multiple regulatory modules contribute to the complex expression programs realized by many loci. Although long thought of as isolated components, recent studies demonstrate that such regulatory sequences can physically associate with promoters and with each other and may localize to specific sub-nuclear transcription ...

journal_title：Nucleic acids research

authors： Dib S,Denarier E,Dionne N,Beaudoin M,Friedman HH,Peterson AC

更新日期：2011-04-01 00:00:00

abstract：:G-quadruplexes (G4s) are stable secondary structures that can lead to the stalling of replication forks and cause genomic instability. Pif1 is a 5' to 3' helicase, localized to both the mitochondria and nucleus that can unwind G4s in vitro and prevent fork stalling at G4 forming sequences in vivo. Using in vitro prime...

journal_title：Nucleic acids research

authors： Sparks MA,Singh SP,Burgers PM,Galletto R

更新日期：2019-09-19 00:00:00

abstract：:In this paper, we report the cloning and sequencing of the C. elegans histidyl-tRNA synthetase gene. The complete genomic sequence, and most of the cDNA sequence, of this gene is now determined. The gene size including flanking and coding regions is 2230 nucleotides long. Three small introns (45-50 bp long) are found ...

journal_title：Nucleic acids research

authors： Amaar YG,Baillie DL

更新日期：1993-09-11 00:00:00

abstract：:Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mu...

journal_title：Nucleic acids research

authors： Kohler SW,Provost GS,Kretz PL,Dycaico MJ,Sorge JA,Short JM

更新日期：1990-05-25 00:00:00

abstract：:We have investigated the mechanism of the electrophoresis-driven chromatin aggregation which had been described by Weintraub (1984, Cell 38, 17-27) as a putative mean for propagation of genetic repression in eukaryotes. We show that the oligonucleosome aggregates are assembled de novo at the starting zone of DNP elect...

journal_title：Nucleic acids research

authors： Grigoryev SA,Spirin KS,Krasheninnikov IA

更新日期：1990-12-25 00:00:00

abstract：:The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1976-12-01 00:00:00

abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...

journal_title：Nucleic acids research

authors： Kuo LY,Cech TR

更新日期：1996-10-01 00:00:00

abstract：:The Escherichia coli fmu gene product has recently been determined to be the 16S rRNA m(5)C 967 methyltransferase. As such, Fmu represents the first protein identified as an S -adenosyl-L-methionine (AdoMet)- dependent RNA m(5)C methyltransferase whose amino acid sequence is known. Using the amino acid sequence of Fmu...

journal_title：Nucleic acids research

authors： Reid R,Greene PJ,Santi DV

更新日期：1999-08-01 00:00:00

abstract：:Several plasmids which direct the expression of human interferon-gamma (IFN-gamma) cDNA in the yeast Saccharomyces cerevisiae under the transcriptional control of the 3-phosphoglycerate kinase (PGK) promoter have been constructed. Up to 25 x 10(6) units per liter culture of biologically active IFN-gamma are synthesize...

journal_title：Nucleic acids research

authors： Derynck R,Singh A,Goeddel DV

更新日期：1983-03-25 00:00:00

abstract：:Knowledge of the various interactions between molecules in the cell is crucial for understanding cellular processes in health and disease. Currently available interaction databases, being largely complementary to each other, must be integrated to obtain a comprehensive global map of the different types of interactions...

journal_title：Nucleic acids research

authors： Kamburov A,Stelzl U,Lehrach H,Herwig R

更新日期：2013-01-01 00:00:00

abstract：:Two different genomic genes for the COUP-transcription factor, COUP-TF I and COUP-TF II, have been isolated from a human cosmid genomic library using a [32P]-labeled cDNA probe. Data obtained from Southern blot analysis of these cosmid clones indicated that two closely related genes exist in the human genome and have ...

journal_title：Nucleic acids research

authors： Ritchie HH,Wang LH,Tsai S,O'Malley BW,Tsai MJ

更新日期：1990-12-11 00:00:00

abstract：:Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in ...

journal_title：Nucleic acids research

authors： Popenda M,Milecki J,Adamiak RW

更新日期：2004-08-03 00:00:00

abstract：:Ribosome biogenesis is fundamental for cellular life, but surprisingly little is known about the underlying pathway. In eukaryotes a comprehensive collection of experimentally verified ribosome biogenesis factors (RBFs) exists only for Saccharomyces cerevisiae. Far less is known for other fungi, animals or plants, and...

journal_title：Nucleic acids research

authors： Ebersberger I,Simm S,Leisegang MS,Schmitzberger P,Mirus O,von Haeseler A,Bohnsack MT,Schleiff E

更新日期：2014-02-01 00:00:00

abstract：:Nuclear DNA binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein Oct-1. It binds to octamer related sequences from the enhancer of human papillomavirus type 18. The activity and intracellular distribution of p92 is regulated by ext...

journal_title：Nucleic acids research

authors： Weitz J,Kopun M,Stoehr M,Napierski I,Royer HD

更新日期：1991-10-25 00:00:00

abstract：:The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiat...

journal_title：Nucleic acids research

authors： Okamoto T,Sugimoto K,Sugisaki H,Takanami M

更新日期：1977-07-01 00:00:00

abstract：:SUPERFAMILY provides structural, functional and evolutionary information for proteins from all completely sequenced genomes, and large sequence collections such as UniProt. Protein domain assignments for over 900 genomes are included in the database, which can be accessed at http://supfam.org/. Hidden Markov models ba...

journal_title：Nucleic acids research

authors： Wilson D,Pethica R,Zhou Y,Talbot C,Vogel C,Madera M,Chothia C,Gough J

更新日期：2009-01-01 00:00:00

abstract：:The Retinol-Binding Protein (RBP) is expressed primarily in the liver. The regulatory elements involved in its tissue-specific expression have been identified and mapped to the 5' flanking region of the RBP gene. In this paper heterokaryons and somatic cell-hybrids have been produced and analysed in order to demonstra...

journal_title：Nucleic acids research

authors： Faraonio R,Musy M,Colantuoni V

更新日期：1990-12-25 00:00:00

abstract：:Patterns in biological sequences frequently signify interesting features in the underlying molecule. Many tools exist to search for well-known patterns. Less support is available for exploratory analysis, where no well-defined patterns are known yet. PatScanUI (https://patscan.secondarymetabolites.org/) provides a hig...

journal_title：Nucleic acids research

authors： Blin K,Wohlleben W,Weber T

更新日期：2018-07-02 00:00:00

abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

journal_title：Nucleic acids research

authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

更新日期：2014-04-01 00:00:00

abstract：:Polyaromatic molecules with amino chain substituents, upon binding with DNA, selectively catalyze exchange of the A.T base pair protons with bulk water protons. The amine-catalyzed exchange is mediated by compounds which are A.T and G.C base sequence specific, intercalators, and outside binders. A mechanism for the se...

journal_title：Nucleic acids research

authors： Strekowski L,Watson RA,Wilson WD

更新日期：1987-10-26 00:00:00

abstract：:A highly polymorphic locus associated with the variable tandem repetition of a 35 bp consensus sequence was mapped to chromosome 10, band q26. Examination of leukocyte DNA from a cancer patient revealed the twenty-fold amplification of one allelic fragment of this locus, while the other allelic fragment demonstrated a...

journal_title：Nucleic acids research

authors： Colb M,Yang-Feng T,Francke U,Mermer B,Parkinson DR,Krontiris TG

更新日期：1986-10-24 00:00:00

abstract：:The enterovirus 71 (EV71) 3Dpol is an RNA-dependent RNA polymerase (RdRP) that plays the central role in the viral genome replication, and is an important target in antiviral studies. Here, we report a crystal structure of EV71 3Dpol elongation complex (EC) at 1.8 Å resolution. The structure reveals that the 5'-end gu...

journal_title：Nucleic acids research

authors： Shi W,Ye HQ,Deng CL,Li R,Zhang B,Gong P

更新日期：2020-02-20 00:00:00

abstract：:Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination fa...

journal_title：Nucleic acids research

authors： Yue L,Li J,Zhang B,Qi L,Li Z,Zhao F,Li L,Zheng X,Dong X

更新日期：2020-09-25 00:00:00

abstract：:Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication...

journal_title：Nucleic acids research

authors： Grainger WH,Machón C,Scott DJ,Soultanas P

更新日期：2010-05-01 00:00:00

abstract：:A method is presented for the purification of mRNAs and the identification of structural gene sequences in recombinant DNA molecules. RNA is hybridized to double-stranded linear DNA such that R-loops are formed between most DNAs and their complementary RNA sequences. These R-loops are purified from unhybridized RNAs b...

journal_title：Nucleic acids research

authors： Woolford JL Jr,Rosbash M

更新日期：1979-06-11 00:00:00

abstract：:The high-mobility-group A2 protein (HMGA2) plays important functional roles in transcriptional regulation, DNA replication and chromatin structure. In this study, the effect of histone deacetylase inhibition on the transcriptional activity of the Hmga2 gene was investigated in vivo both at the endogenous gene level an...

journal_title：Nucleic acids research

authors： Ferguson M,Henry PA,Currie RA

更新日期：2003-06-15 00:00:00

abstract：:A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes ...

journal_title：Nucleic acids research

authors： Laslett D,Canback B,Andersson S

更新日期：2002-08-01 00:00:00

abstract：:We have determined the stability of intramolecular DNA quadruplexes in which the four G(3)-tracts are connected by non-nucleosidic linkers containing propanediol, octanediol or hexaethylene glycol, replacing the TTA loops in the human telomeric repeat sequence. We find that these sequences all fold to form intramolecu...

journal_title：Nucleic acids research

authors： Risitano A,Fox KR

更新日期：2004-05-11 00:00:00

abstract：:The DINAMelt web server simulates the melting of one or two single-stranded nucleic acids in solution. The goal is to predict not just a melting temperature for a hybridized pair of nucleic acids, but entire equilibrium melting profiles as a function of temperature. The two molecules are not required to be complementa...

journal_title：Nucleic acids research

authors： Markham NR,Zuker M

更新日期：2005-07-01 00:00:00