Abstract:
:Nuclear DNA binding protein p92 is a sequence specific octamer binding protein with identical molecular weight as the ubiquitous octamer binding protein Oct-1. It binds to octamer related sequences from the enhancer of human papillomavirus type 18. The activity and intracellular distribution of p92 is regulated by extracellular signals. In serum starved Hela-fibroblast hybrid cells p92 is localized to the cytosol. Serum stimulation leads to nuclear import of p92. In fractions of asynchronously growing cells, which were separated according to cell cycle phases into G1, S, and G2 populations by centrifugal elutriation, p92 DNA binding is confined to S phase. In binding site blots however, p92 DNA binding activity is also present in G1 and G2. In G1 and G2 DNA binding activity of p92 is masked by a novel nuclear inhibitor I-92. The cyclic association of p92 with its inhibitor I-92 provides a new mechanism of regulating S phase dependent activity of a sequence specific DNA binding protein.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Weitz J,Kopun M,Stoehr M,Napierski I,Royer HDdoi
10.1093/nar/19.20.5725subject
Has Abstractpub_date
1991-10-25 00:00:00pages
5725-30issue
20eissn
0305-1048issn
1362-4962journal_volume
19pub_type
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