Abstract:
:Three clones containing both the human G gamma and A gamma globlin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5' most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the gamma gene on the opposite chromosome. Restriction analysis of the three clones indicates that the G gamma and A gamma genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kg between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the gamma genes in man.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ramirez F,Burns AL,Mears JG,Spence S,Starkman D,Bank Adoi
10.1093/nar/7.5.1147subject
Has Abstractpub_date
1979-11-10 00:00:00pages
1147-62issue
5eissn
0305-1048issn
1362-4962journal_volume
7pub_type
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