Abstract:
:Base specificity in the interaction of ethidium with double stranded synthetic RNA homopolymers has been studied by means of spectroscopic (UV-visible absorption and fluorescence), microcalorimetric and dilatometric techniques. The results show a strong base specificity in this interaction, the association constant with poly A:poly U being more than three order of magnitude higher than with poly O:poly C. The interaction is mainly enthalpy driven, the differences in affinity being essentially entropic in origin. These evidences along with the dilatometric data suggest that the observed base specificity may arise from the different extent of water release upon intercalation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Babayan Y,Manzini G,Xodo LE,Quadrifoglio Fdoi
10.1093/nar/15.14.5803subject
Has Abstractpub_date
1987-07-24 00:00:00pages
5803-12issue
14eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::Potential pathways that could account for observed rapid rates of transcription reinitiation were explored. A nuclear extract system was established in which reinitiation rates were observed to be kinetically facilitated and in which the rate was sensitive to TATA box mutation. Kinetic facilitation of functional compl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.3.831
更新日期:1999-02-01 00:00:00
abstract::Assessment of the fidelity of gene expression is crucial to understand cell homeostasis. Here we present a highly sensitive method for the systematic Quantification of Rare Amino acid Substitutions (QRAS) using absolute quantification by targeted mass spectrometry after chromatographic enrichment of peptides with miss...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment whi...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.20.4709
更新日期:1980-10-24 00:00:00
abstract::4'-Acylated thymidines represent a new class of DNA chain terminators, since they have been shown to act as post-incorporation chain-terminating nucleotides despite the presence of a free 3'-hydroxyl group. Here, we describe the action of the 4'-acetyl- (MeTTP) and 4'-propanoylthymidine 5'-triphosphate (EtTTP) on HIV-...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.12.5067
更新日期:1986-06-25 00:00:00
abstract::DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...
journal_title:Nucleic acids research
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更新日期:1977-04-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2002-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz251
更新日期:2019-06-20 00:00:00
abstract::A computer program package has been made available which contains five compound programs written in FORTRAN 10 (for DECsystem-10). Program DATBAS is for storing and improving DNA sequence data, especially those obtained by the sequencing method using M13 phages. Programs NUCDAT and PROTEN are for analyzing DNA/RNA and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part1.101
更新日期:1984-01-11 00:00:00
abstract::Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...
journal_title:Nucleic acids research
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更新日期:2010-03-01 00:00:00
abstract::The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. To address this need, we developed a robust strategy for evolving site-specific recombinases with novel substrate specificities. In this system, recombi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq125
更新日期:2010-07-01 00:00:00
abstract::Mammalian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation. Phosphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication. Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. The ...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-02-29 00:00:00
abstract::Simple modular architecture research tool (SMART) is an online tool (http://smart.embl.de/) for the identification and annotation of protein domains. It provides a user-friendly platform for the exploration and comparative study of domain architectures in both proteins and genes. The current release of SMART contains ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn808
更新日期:2009-01-01 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkh002
更新日期:2004-01-01 00:00:00
abstract::We have created a database of comparatively derived group I intron secondary structure diagrams. This collection currently contains a broad sampling of phylogenetically and structurally similar and diverse structures from over 200 publicly available intron sequences. As more group I introns are sequenced and added to ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.17.3508
更新日期:1994-09-01 00:00:00
abstract::Based on the secondary structural model of MS2 RNA, it is shown that, in base-pairing regions of the RNA, there is a bias in the use of synonymous codons which favours C and/or G over U and/or A in the third codon positions, and that in non-pairing regions, there is an opposite bias which favours U and/or A over C and...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1979-12-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2001-07-15 00:00:00
abstract::The sxy (tfoX) gene product is the central regulator of DNA uptake by naturally competent gamma-proteobacteria such as Haemophilus influenzae, Vibrio cholerae and probably Escherichia coli. However, the mechanisms regulating sxy gene expression are not understood despite being key to understanding the physiological ro...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1987-10-26 00:00:00
abstract::The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-04-23 00:00:00
abstract::We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves of Digitaria setigera. This study has confirmed that both spliced and unspliced RNAs are synthesised by the same transcription unit. The position of the intron...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7259
更新日期:1990-12-25 00:00:00
abstract::DNA polymerase delta (Pol δ) is responsible for the elongation and maturation of Okazaki fragments in eukaryotic cells. Proliferating cell nuclear antigen (PCNA) recruits Pol δ to the DNA and serves as a processivity factor. Here, we show that PCNA also stimulates the catalytic rate of Saccharomyces cerevisiae Pol δ b...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-02-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1037
更新日期:2014-02-01 00:00:00
abstract::To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.22.8931
更新日期:1989-11-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1974-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.2.493
更新日期:1979-09-25 00:00:00
abstract::The Protein Circular Dichroism Data Bank (PCDDB) has been in operation for more than 5 years as a public repository for archiving circular dichroism spectroscopic data and associated bioinformatics and experimental metadata. Since its inception, many improvements and new developments have been made in data display, se...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw796
更新日期:2017-01-04 00:00:00