Abstract:
:The lambdagt clones containing fragments of the Drosophila melanogaster genome were prepared and characterized by hybridization of their DNA with (I) lambdagt-cRNA; (2) lambdaC-cRNA; (3) Dm-cRNA; (4) the mRNA of D.melanogaster culture cells and (5) the stable cytoplasmic poly (A) RNA from the same source. The technique for a simple selection of hybrid clones is described. The hybridization with mRNA allows one to select the clones containing structural genes of D.melanogaster. It was found that in all cases when the clone contains the structural gene it also contains the reiterated base sequences of the D.melanogaster genome. Several clones containing D. melanogaster DNA fragments with a size of (2-4)x1O6 daltons hybridizing with a relatively large portion of mRNA were selected for further analysis.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ilyin YV,Tchurikov NA,Georgievdoi
10.1093/nar/3.8.2115subject
Has Abstractpub_date
1976-08-01 00:00:00pages
2115-27issue
8eissn
0305-1048issn
1362-4962journal_volume
3pub_type
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