'Solo' large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome.

abstract：:Bacterial small RNAs (sRNAs) are a heterogeneous group of post-transcriptional regulators that often act at the heart of large networks. Hundreds of sRNAs have been discovered by genome-wide screens and most of these sRNAs exert their functions by base-pairing with target mRNAs. However, studies addressing the molecul...

journal_title：Nucleic acids research

authors： Fröhlich KS,Förstner KU,Gitai Z

更新日期：2018-11-16 00:00:00

abstract：:Members of the HMG-I(Y) family of mammalian nonhistone proteins are of importance because they have been demonstrated to bind specifically to the minor groove of A.T-rich sequences both in vitro and in vivo and to function as gene transcriptional regulatory proteins in vivo. Here we report the cloning, sequencing, cha...

journal_title：Nucleic acids research

authors： Friedmann M,Holth LT,Zoghbi HY,Reeves R

更新日期：1993-09-11 00:00:00

abstract：:Computational modelling of mammalian cell cycle regulation is a challenging task, which requires comprehensive knowledge on many interrelated processes in the cell. We have developed a web-based integrated database on cell cycle regulation in mammals in normal and pathological states (Cyclonet database). It integrates...

journal_title：Nucleic acids research

authors： Kolpakov F,Poroikov V,Sharipov R,Kondrakhin Y,Zakharov A,Lagunin A,Milanesi L,Kel A

更新日期：2007-01-01 00:00:00

abstract：:Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...

journal_title：Nucleic acids research

authors： Yue M,Ogawa Y

更新日期：2018-03-16 00:00:00

abstract：:We report the first isolation and characterisation of a human Ig V lambda gene. The gene was isolated from a recombinant phage library of human chromosome 22 using a mouse Ig lambda cDNA as probe. DNA sequence analysis predicts a short leader peptide interrupted by an intron of 88 nucleotides, and a mature polypeptide...

journal_title：Nucleic acids research

authors： Anderson ML,Szajnert MF,Kaplan JC,McColl L,Young BD

更新日期：1984-09-11 00:00:00

abstract：:Peptide nucleic acids (PNAs) can bind to homopurine/homopyrimidine sequences of double-stranded DNA targets in a sequence-specific manner and form [PNA]2/DNA triplexes with single-stranded DNA D-loop structures at the PNA binding sites. These D-loop structures have been found to have a capacity to initiate transcripti...

journal_title：Nucleic acids research

authors： Wang G,Xu X,Pace B,Dean DA,Glazer PM,Chan P,Goodman SR,Shokolenko I

更新日期：1999-07-01 00:00:00

abstract：:We have created a database of comparatively derived group I intron secondary structure diagrams. This collection currently contains a broad sampling of phylogenetically and structurally similar and diverse structures from over 200 publicly available intron sequences. As more group I introns are sequenced and added to ...

journal_title：Nucleic acids research

authors： Damberger SH,Gutell RR

更新日期：1994-09-01 00:00:00

abstract：:The human Microprocessor complex cleaves primary microRNA (miRNA) transcripts (pri-miRNAs) to initiate miRNA synthesis. Microprocessor consists of DROSHA (an RNase III enzyme), and DGCR8. DROSHA contains two RNase III domains, RIIIDa and RIIIDb, which simultaneously cleave the 3p- and 5p-strands of pri-miRNAs, respect...

journal_title：Nucleic acids research

authors： Nguyen TL,Nguyen TD,Bao S,Li S,Nguyen TA

更新日期：2020-03-18 00:00:00

abstract：:Algae are a diverse, polyphyletic group of photosynthetic eukaryotes spanning nearly all eukaryotic lineages of life and collectively responsible for ∼50% of photosynthesis on Earth. Sequenced algal genomes, critical to understanding their complex biology, are growing in number and require efficient tools for analysis...

journal_title：Nucleic acids research

authors： Grigoriev IV,Hayes RD,Calhoun S,Kamel B,Wang A,Ahrendt S,Dusheyko S,Nikitin R,Mondo SJ,Salamov A,Shabalov I,Kuo A

更新日期：2021-01-08 00:00:00

abstract：:We present a www server for AUGUSTUS, a novel software program for ab initio gene prediction in eukaryotic genomic sequences. Our method is based on a generalized Hidden Markov Model with a new method for modeling the intron length distribution. This method allows approximation of the true intron length distribution m...

journal_title：Nucleic acids research

authors： Stanke M,Steinkamp R,Waack S,Morgenstern B

更新日期：2004-07-01 00:00:00

abstract：:By subtractive screening of a library made from mRNA of lipopolysaccharide (LPS)-stimulated mouse B lymphocytes we isolated cDNA-clones encoding the ribosomal protein L7. Human L7 mRNA was cloned from activated T-lymphocytes. Although no specific function of L7 in the translation apparatus is known as yet, it should b...

journal_title：Nucleic acids research

authors： Hemmerich P,von Mikecz A,Neumann F,Sözeri O,Wolff-Vorbeck G,Zoebelein R,Krawinkel U

更新日期：1993-01-25 00:00:00

abstract：:Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulg...

journal_title：Nucleic acids research

authors： Kuznetsova IL,Zenkova MA,Gross HJ,Vlassov VV

更新日期：2005-02-24 00:00:00

abstract：:We describe mGene.web, a web service for the genome-wide prediction of protein coding genes from eukaryotic DNA sequences. It offers pre-trained models for the recognition of gene structures including untranslated regions in an increasing number of organisms. With mGene.web, users have the additional possibility to tr...

journal_title：Nucleic acids research

authors： Schweikert G,Behr J,Zien A,Zeller G,Ong CS,Sonnenburg S,Rätsch G

更新日期：2009-07-01 00:00:00

abstract：:ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicy...

journal_title：Nucleic acids research

authors： Kumarevel T,Tanaka T,Umehara T,Yokoyama S

更新日期：2009-08-01 00:00:00

abstract：:Transcription factors (TFs) regulate gene expression by binding to short DNA sequence motifs, yet their binding specificities alone cannot explain how certain TFs drive a diversity of biological processes. In order to investigate the factors that control the functions of the pleiotropic TF STAT3, we studied its genome...

journal_title：Nucleic acids research

authors： Hutchins AP,Diez D,Takahashi Y,Ahmad S,Jauch R,Tremblay ML,Miranda-Saavedra D

更新日期：2013-02-01 00:00:00

abstract：:We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes an...

journal_title：Nucleic acids research

authors： Wang ML,Leitch AR,Schwarzacher T,Heslop-Harrison JS,Moore G

更新日期：1992-04-25 00:00:00

abstract：:Increasing experimental evidence shows a prominent role of histone modifications in the coordinated control of gene expression in the human malaria parasite Plasmodium falciparum. The search for the histone-mark-reading machinery that translates histone modifications into biological processes, such as formation of het...

journal_title：Nucleic acids research

authors： Pérez-Toledo K,Rojas-Meza AP,Mancio-Silva L,Hernández-Cuevas NA,Delgadillo DM,Vargas M,Martínez-Calvillo S,Scherf A,Hernandez-Rivas R

更新日期：2009-05-01 00:00:00

abstract：:We have used temperature gradient gel electrophoresis (TGGE) to measure the progress of local denaturation in closed circular topoisomer DNA as a function of temperature and superhelicity (sigma). We describe the versatility of this method as a tool for detecting various conformational modifications of plasmid DNAs. T...

journal_title：Nucleic acids research

authors： Víglaský V,Antalík M,Adamcík J,Podhradský D

更新日期：2000-06-01 00:00:00

abstract：:The role of thymine dimerization for previously reported U.V. induced conformational changes has been proved using acetophenone as sensitizer for the specific thymine dimerization upon irradiation at 313 nm. CD results demonstrate that formation of pyrimidine dimers cause typical conformational changes of the DNA B he...

journal_title：Nucleic acids research

authors： Lang H

更新日期：1975-02-01 00:00:00

abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...

journal_title：Nucleic acids research

authors： Flybase Consortium.

更新日期：1996-01-01 00:00:00

abstract：:Beta-thalassemia is a genetic disorder caused by mutations in the beta-globin gene. Triplex-forming oligonucleotides and triplex-forming peptide nucleic acids (PNAs) have been shown to stimulate recombination in mammalian cells via site-specific binding and creation of altered helical structures that provoke DNA repai...

journal_title：Nucleic acids research

authors： Lonkar P,Kim KH,Kuan JY,Chin JY,Rogers FA,Knauert MP,Kole R,Nielsen PE,Glazer PM

更新日期：2009-06-01 00:00:00

abstract：:Optogenetic control of CRISPR-Cas9 systems has significantly improved our ability to perform genome perturbations in living cells with high precision in time and space. As new Cas orthologues with advantageous properties are rapidly being discovered and engineered, the need for straightforward strategies to control th...

journal_title：Nucleic acids research

authors： Hoffmann MD,Mathony J,Upmeier Zu Belzen J,Harteveld Z,Aschenbrenner S,Stengl C,Grimm D,Correia BE,Eils R,Niopek D

更新日期：2020-12-16 00:00:00

abstract：:We have located and sequenced the murine homologue of the hst/k-FGF oncogene in genomic DNA clones that extend 3' from int-2 on mouse chromosome 7. The two genes are in the same transcriptional orientation, less than 20 kilobase pairs (kb) apart, and presumably evolved by tandem duplication of a common ancestral gene....

journal_title：Nucleic acids research

authors： Brookes S,Smith R,Thurlow J,Dickson C,Peters G

更新日期：1989-06-12 00:00:00

abstract：:A theoretical approach for linkage mapping the genome of any higher eukaryote is described. It uses the polymerase chain reaction, oligonucleotides of random sequence and single haploid cells. Markers are defined and then the DNA of a single sperm is broken at random (eg by gamma-rays) and physically split into 3 aliq...

journal_title：Nucleic acids research

authors： Dear PH,Cook PR

更新日期：1989-09-12 00:00:00

abstract：:The E. coli single-stranded binding protein (SSB) has been demonstrated in vitro to be involved in a number of replicative, DNA renaturation, and protective functions. It was shown previously that SSB can interact with exonuclease I to stimulate the hydrolysis of single-stranded DNA. We demonstrate here that E. coli S...

journal_title：Nucleic acids research

authors： Sandigursky M,Franklin WA

更新日期：1994-01-25 00:00:00

abstract：:Many mutations occur as a result of DNA synthesis past the site of DNA damage by DNA damage bypass polymerases. The frequency and types of mutations not only depend on the nature of the damage, but also on the sequence context, as revealed from analysis of mutation spectra of DNA exposed to mutagens. Herein we report ...

journal_title：Nucleic acids research

authors： Fang H,Taylor JS

更新日期：2008-10-01 00:00:00

abstract：:In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously s...

journal_title：Nucleic acids research

authors： Bostian KA,Burn VE,Jayachandran S,Tipper DJ

更新日期：1983-02-25 00:00:00

abstract：:The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery. This is achieved, in part, via the co-ordinated regulation of mRNAs that contain a terminal ...

journal_title：Nucleic acids research

authors： Smith EM,Benbahouche NEH,Morris K,Wilczynska A,Gillen S,Schmidt T,Meijer HA,Jukes-Jones R,Cain K,Jones C,Stoneley M,Waldron JA,Bell C,Fonseca BD,Blagden S,Willis AE,Bushell M

更新日期：2021-01-11 00:00:00

abstract：:A simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage M13. The method rests on gapped duplex DNA (gdDNA) molecules of the phage M13 genome as the key intermediate. In this gdDNA, the (+) and the (shorter) (-) strand carry different g...

journal_title：Nucleic acids research

authors： Kramer W,Drutsa V,Jansen HW,Kramer B,Pflugfelder M,Fritz HJ

更新日期：1984-12-21 00:00:00

abstract：:The RNA sequences boxA, boxB and boxC constitute the nut regions of phage lambda. They nucleate the formation of a termination-resistant RNA polymerase complex on the lambda chromosome. The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein. A complex that includes the Nus proteins a...

journal_title：Nucleic acids research

authors： Prasch S,Jurk M,Washburn RS,Gottesman ME,Wöhrl BM,Rösch P

更新日期：2009-08-01 00:00:00