Abstract:
:We have located and sequenced the murine homologue of the hst/k-FGF oncogene in genomic DNA clones that extend 3' from int-2 on mouse chromosome 7. The two genes are in the same transcriptional orientation, less than 20 kilobase pairs (kb) apart, and presumably evolved by tandem duplication of a common ancestral gene. RNase mapping and primer extension analyses indicated that the major 3.2 kb hst transcript expressed in undifferentiated embryonal carcinoma (EC) cell lines initiates at a unique cap site downstream from an obvious TATA-box. The 3' end of the transcript as identified in multiple cDNA clones occurs at an appropriate distance from a variant polyadenylation signal, ATTAAA. Translation of the major open reading frame would yield a 202 amino acid protein that is 82% homologous to human HST but lacking 4 residues at the presumed signal peptide cleavage site.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Brookes S,Smith R,Thurlow J,Dickson C,Peters Gdoi
10.1093/nar/17.11.4037subject
Has Abstractpub_date
1989-06-12 00:00:00pages
4037-45issue
11eissn
0305-1048issn
1362-4962journal_volume
17pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1978-01-01 00:00:00
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journal_title:Nucleic acids research
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abstract::MicA is a trans-encoded small non-coding RNA, which downregulates porin-expression in stationary-phase. In this work, we focus on the role of endoribonucleases III and E on Salmonella typhimurium sRNA MicA regulation. RNase III is shown to regulate MicA in a target-coupled way, while RNase E is responsible for the con...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00