c-MYC empowers transcription and productive splicing of the oncogenic splicing factor Sam68 in cancer.

abstract：:Bile acid biosynthesis is subjected to feedback regulation whereby bile acids down-regulate their own synthesis. The major point of this regulation is at the level of cholesterol 7alpha-hydroxylase (7alpha-hydroxylase), which controls bile acid output from the classic pathway. This regulation is at the level of transc...

journal_title：Nucleic acids research

authors： del Castillo-Olivares A,Gil G

更新日期：2000-09-15 00:00:00

abstract：:Biological pathways can provide key information on the organization of biological systems. ArrayXPath (http://www.snubi.org/software/ArrayXPath/) is a web-based service for mapping and visualizing microarray gene-expression data for integrated biological pathway resources using Scalable Vector Graphics (SVG). By integ...

journal_title：Nucleic acids research

authors： Chung HJ,Kim M,Park CH,Kim J,Kim JH

更新日期：2004-07-01 00:00:00

abstract：:Single-cell RNA-sequencing (scRNA-seq) enables the characterization of transcriptomic profiles at the single-cell resolution with increasingly high throughput. However, it suffers from many sources of technical noises, including insufficient mRNA molecules that lead to excess false zero values, termed dropouts. Comput...

journal_title：Nucleic acids research

authors： Xu Y,Zhang Z,You L,Liu J,Fan Z,Zhou X

更新日期：2020-09-04 00:00:00

abstract：:Topoisomerase II is an essential enzyme that is required for virtually every process that requires movement of DNA within the nucleus or the opening of the double helix. This enzyme helps to regulate DNA under- and overwinding and removes knots and tangles from the genetic material. In order to carry out its critical ...

journal_title：Nucleic acids research

authors： Deweese JE,Osheroff N

更新日期：2009-02-01 00:00:00

abstract：:DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...

journal_title：Nucleic acids research

authors： Laquel P,Castroviejo M,Litvak S

更新日期：1990-08-25 00:00:00

abstract：:Fundamental aspects of the biochemistry of Type III restriction endonucleases remain unresolved despite being characterized by numerous research groups in the past decades. One such feature is the subunit stoichiometry of these hetero-oligomeric enzyme complexes, which has important implications for the reaction mecha...

journal_title：Nucleic acids research

authors： Butterer A,Pernstich C,Smith RM,Sobott F,Szczelkun MD,Tóth J

更新日期：2014-04-01 00:00:00

abstract：:Many tools are available to analyse genomes but are often challenging to use in a cell type-specific context. We have developed a method similar to the isolation of nuclei tagged in a specific cell type (INTACT) technique [Deal,R.B. and Henikoff,S. (2010) A simple method for gene expression and chromatin profiling of ...

journal_title：Nucleic acids research

authors： Henry GL,Davis FP,Picard S,Eddy SR

更新日期：2012-10-01 00:00:00

abstract：:The Arabidopsis Information Resource (TAIR, http://arabidopsis.org) is the model organism database for the fully sequenced and intensively studied model plant Arabidopsis thaliana. Data in TAIR is derived in large part from manual curation of the Arabidopsis research literature and direct submissions from the research...

journal_title：Nucleic acids research

authors： Swarbreck D,Wilks C,Lamesch P,Berardini TZ,Garcia-Hernandez M,Foerster H,Li D,Meyer T,Muller R,Ploetz L,Radenbaugh A,Singh S,Swing V,Tissier C,Zhang P,Huala E

更新日期：2008-01-01 00:00:00

abstract：:THY Pol-2 intein, from Thermococcus hydrothermalis, belongs to the same allelic family as TLI Pol-2 (PI-TLII), Tfu Pol-2 (PI-TFUII) and TspTY Pol-3 mini-intein, all inserted at the pol-c site of archaeal DNA polymerase genes. This new intein was cloned, expressed in Escherichia coli and purified. The intein is a speci...

journal_title：Nucleic acids research

authors： Saves I,Eleaume H,Dietrich J,Masson JM

更新日期：2000-11-01 00:00:00

abstract：:O-GLYCBASE is a revised database of information on glycoproteins and their O-linked glycosylation sites. Entries are compiled and revised from the literature, and from the sequence databases. Entries include information about species, sequence, glycosylation sites and glycan type and is fully cross-referenced. Compare...

journal_title：Nucleic acids research

authors： Hansen JE,Lund O,Nilsson J,Rapacki K,Brunak S

更新日期：1998-01-01 00:00:00

abstract：:An efficient procedure for the synthesis of unnatural alpha-anomeric oligodeoxyribonucleotides is described. This solid-phase procedure is based on the use of alpha-nucleoside phosphoramidites and alpha-nucleoside derivatized solid supports corresponding to the four natural bases and allow rapid synthesis of oligonucl...

journal_title：Nucleic acids research

authors： Morvan F,Rayner B,Leonetti JP,Imbach JL

更新日期：1988-02-11 00:00:00

abstract：:7SK snRNA, an abundant RNA discovered in human nucleus, regulates transcription by RNA polymerase II (RNAPII). It sequesters and inhibits the transcription elongation factor P-TEFb which, by phosphorylation of RNAPII, switches transcription from initiation to processive elongation and relieves pauses of transcription....

journal_title：Nucleic acids research

authors： Lebars I,Martinez-Zapien D,Durand A,Coutant J,Kieffer B,Dock-Bregeon AC

更新日期：2010-11-01 00:00:00

abstract：:Altering zinc bioavailability to bacterial pathogens is a key component of host innate immunity. Thus, the ability to sense and adapt to the alterations in zinc concentrations is critical for bacterial survival and pathogenesis. To understand the adaptive responses of group A Streptococcus (GAS) to zinc limitation and...

journal_title：Nucleic acids research

authors： Sanson M,Makthal N,Flores AR,Olsen RJ,Musser JM,Kumaraswami M

更新日期：2015-01-01 00:00:00

abstract：:Here we report that the poor binding of methylphosphonate oligodeoxynucleosides (MP-ODNs) to their nucleic acid targets can be improved by additional inversion of the anomeric configuration (from beta to alpha) in the sugar moieties to give a new class of analogs, MP alpha-oligonucleosides. MP alpha-dT12and MP 5' alph...

journal_title：Nucleic acids research

authors： Debart F,Meyer A,Vasseur JJ,Rayner B

更新日期：1998-10-15 00:00:00

abstract：:The promoter for the major coat protein gene of bacteriophage fd contains a unique sequence. TATAAT, in the non-transcribed region corresponding to the Pribnow box. A R-Hha I cleavage site which destroys functions is located five pairs upstream from the TATAAT sequence (fifteen base pairs upstream from the RNA initiat...

journal_title：Nucleic acids research

authors： Okamoto T,Sugimoto K,Sugisaki H,Takanami M

更新日期：1977-07-01 00:00:00

abstract：:Hybridization solutions containing chaotropes may be used to modulate the thermal stability (Tm or Td) of oligodeoxynucleotide (ODN) duplexes or hybrids over a 90 degrees C range. Modulation of Td allows formulation of hybridization solutions that permit ambient temperature hybridization using most combinations of pro...

journal_title：Nucleic acids research

authors： Van Ness J,Chen L

更新日期：1991-10-11 00:00:00

abstract：:The E3 ubiquitin ligase HUWE1/Mule/ARF-BP1 plays an important role in integrating/coordinating diverse cellular processes such as DNA damage repair and apoptosis. A previous study has shown that HUWE1 is required for the early step of DNA damage-induced apoptosis, by targeting MCL-1 for proteasomal degradation. Howeve...

journal_title：Nucleic acids research

authors： Yi J,Lu G,Li L,Wang X,Cao L,Lin M,Zhang S,Shao G

更新日期：2015-05-19 00:00:00

abstract：:Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This 'read-through' is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence o...

journal_title：Nucleic acids research

authors： Harrell L,Melcher U,Atkins JF

更新日期：2002-05-01 00:00:00

abstract：:Toxicogenomics focuses on assessing the safety of compounds using gene expression profiles. Gene expression signatures from large toxicogenomics databases are expected to perform better than small databases in identifying biomarkers for the prediction and evaluation of drug safety based on a compound's toxicological m...

journal_title：Nucleic acids research

authors： Igarashi Y,Nakatsu N,Yamashita T,Ono A,Ohno Y,Urushidani T,Yamada H

更新日期：2015-01-01 00:00:00

abstract：:'Linker scanning' has been used as an efficient method for systematically surveying a segment of DNA for functional elements by mutagenesis. A three-step PCR method was developed to simplify this process. In this method, a set of 'mutation primers' was made with 6 to 8 base substitutions in the center of the primers. ...

journal_title：Nucleic acids research

authors： Li XM,Shapiro LJ

更新日期：1993-08-11 00:00:00

abstract：:The webPDBinder (http://pdbinder.bio.uniroma2.it/PDBinder) is a web server for the identification of small ligand-binding sites in a protein structure. webPDBinder searches a protein structure against a library of known binding sites and a collection of control non-binding pockets. The number of similarities identifie...

journal_title：Nucleic acids research

authors： Bianchi V,Mangone I,Ferrè F,Helmer-Citterich M,Ausiello G

更新日期：2013-07-01 00:00:00

abstract：:A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator complex was determine...

journal_title：Nucleic acids research

authors： Klig LS,Crawford IP,Yanofsky C

更新日期：1987-07-10 00:00:00

abstract：:Exposure to genotoxic agents, such as ionizing radiation (IR), produces double-strand breaks, repaired predominantly in mammalian cells by non-homologous end-joining (NHEJ). Ku70 was identified as an interacting partner of a proteolytic Cyclin E (CycE) fragment, p18CycE. p18CycE endogenous generation during IR-induced...

journal_title：Nucleic acids research

authors： Chatterjee P,Plesca D,Mazumder S,Boutros J,Yannone SM,Almasan A

更新日期：2013-12-01 00:00:00

abstract：:Dnmt3a and Dnmt3b are paralogous enzymes responsible for de novo DNA methylation but with distinguished biological functions. In mice, disruption of Dnmt3b but not Dnmt3a causes global DNA hypomethylation, especially in repetitive sequences, which comprise the large majority of methylated DNA in the genome. By measuri...

journal_title：Nucleic acids research

authors： Shen L,Gao G,Zhang Y,Zhang H,Ye Z,Huang S,Huang J,Kang J

更新日期：2010-10-01 00:00:00

abstract：:Bacterial genomics has revolutionized our understanding of the microbial tree of life; however, mapping and visualizing the distribution of functional traits across bacteria remains a challenge. Here, we introduce AnnoTree-an interactive, functionally annotated bacterial tree of life that integrates taxonomic, phyloge...

journal_title：Nucleic acids research

authors： Mendler K,Chen H,Parks DH,Lobb B,Hug LA,Doxey AC

更新日期：2019-05-21 00:00:00

abstract：:Proper DNA double-strand break (DSB) repair is essential for maintaining genome integrity. Microhomology-mediated end joining (MMEJ) is an error-prone repair mechanism, which introduces mutations at break sites and contributes to chromosomal translocations and telomere fusions, thus driving carcinogenesis. Mitotic kin...

journal_title：Nucleic acids research

authors： Wang H,Qiu Z,Liu B,Wu Y,Ren J,Liu Y,Zhao Y,Wang Y,Hao S,Li Z,Peng B,Xu X

更新日期：2018-11-16 00:00:00

abstract：:The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...

journal_title：Nucleic acids research

authors： Skrzypek MS,Nash RS,Wong ED,MacPherson KA,Hellerstedt ST,Engel SR,Karra K,Weng S,Sheppard TK,Binkley G,Simison M,Miyasato SR,Cherry JM

更新日期：2018-01-04 00:00:00

abstract：:In bacteria, promoter identification by RNA polymerase is mediated by a dissociable σ factor. The housekeeping σ(70) factor of Escherichia coli recognizes two well characterized DNA sequence elements, known as the '-10' and '-35' hexamers. These elements are separated by 'spacer' DNA, the sequence of which is generall...

journal_title：Nucleic acids research

authors： Singh SS,Typas A,Hengge R,Grainger DC

更新日期：2011-07-01 00:00:00

abstract：:An unexpectedly high number of regulatory RNAs have been recently discovered that fine-tune the function of genes at all levels of expression. We employed Genomic SELEX, a method to identify protein-binding RNAs encoded in the genome, to search for further regulatory RNAs in Escherichia coli. We used the global regula...

journal_title：Nucleic acids research

authors： Lorenz C,Gesell T,Zimmermann B,Schoeberl U,Bilusic I,Rajkowitsch L,Waldsich C,von Haeseler A,Schroeder R

更新日期：2010-06-01 00:00:00

abstract：:Short oligo(deoxynucleoside phosphorothioate)s were analyzed as a pool of individual diastereomeric species. The composition of such mixtures, determined by means of HPLC, indicates that consecutive couplings in commonly used phosphoramidite chemistry lead to increasing contents of the Rp isomer. Methods of analysis a...

journal_title：Nucleic acids research

authors： Wilk A,Stec WJ

更新日期：1995-02-11 00:00:00