Abstract:
:In order to facilitate the direct computation of the sizes of DNA fragments separated by gel electrophoresis, we have written and evaluated programmes for the Hewlett-Packard 41C programmable calculator. The sizes estimated for DNA fragments of known length using some of these programmes were found to be more accurate than the estimates obtained by conventional graphical procedures. These programmes should be adaptable to other programmable calculators.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Gough EJ,Gough NMdoi
10.1093/nar/12.1part2.845subject
Has Abstractpub_date
1984-01-11 00:00:00pages
845-53issue
1 Pt 2eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku690
更新日期:2014-09-01 00:00:00
abstract::Reversible methylation of the N6 or N1 position of adenine in RNA has recently been shown to play significant roles in regulating the functions of RNA. RNA can also be alkylated upon exposure to endogenous and exogenous alkylating agents. Here we examined how regio-specific methylation at the hydrogen bonding edge of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx515
更新日期:2017-09-06 00:00:00
abstract::Sequencing of the 3'-untranslated region of the CCT8 gene of the fungal pathogen Candida albicans revealed that the CCT8 coding region overlaps 13 bp with the coding region of the convergently orientated TRP1 gene. The same overlap was found in three strains with different genetic backgrounds. 3'-RACE was used to dete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.22.5061
更新日期:1998-11-15 00:00:00
abstract::High-resolution structures of proteins remain the most valuable source for understanding their function in the cell and provide leads for drug design. Since the availability of sufficient protein structures to tackle complex problems such as modeling backbone moves or docking remains a problem, alternative approaches ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq972
更新日期:2011-01-01 00:00:00
abstract::Simian cells have been transformed with SV40 origin-defective recombinant plasmids containing the tsA209 T-antigen gene. These plasmids contain deletions of either 5 or 52 nucleotides that include the BglI site at the SV40 ori, are defective for replication in COS-1 cells but retain a functional SV40 early promoter. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.22.7913
更新日期:1985-11-25 00:00:00
abstract::The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr914
更新日期:2012-01-01 00:00:00
abstract::The log kobs vs. pH profiles were determined in the intermediate acidity region for the glycosyl hydrolysis of guanosine and its 8-amino, 8-monomethylamino, 8-dimethylamino and 8-bromo derivatives. The decreased rate of the 8-amino and enhanced rate of the 8-bromo compound compared to guanosine support an A type mecha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.3.697
更新日期:1977-03-01 00:00:00
abstract::Signal recognition particle (SRP) is a stable cytoplasmic ribonucleoprotein complex that serves to translocate secretory proteins across membranes during translation. The SRP Database (SRPDB) provides compilations of SRP components, ordered alphabetically and phylogenetically. Alignments emphasize phylogenetically-sup...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.1.169
更新日期:2001-01-01 00:00:00
abstract::The principles of mRNA decoding are conserved among all extant life forms. We present an integrative view of all the interaction networks between mRNA, tRNA and rRNA: the intrinsic stability of codon-anticodon duplex, the conformation of the anticodon hairpin, the presence of modified nucleotides, the occurrence of no...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkw608
更新日期:2016-09-30 00:00:00
abstract::The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.3.332
更新日期:1994-02-11 00:00:00
abstract::Small regulatory RNAs (sRNAs) from bacterial chromosomes became the focus of research over the past five years. However, relatively little is known in terms of structural requirements, kinetics of interaction with their targets and degradation in contrast to well-studied plasmid-encoded antisense RNAs. Here, we presen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm439
更新日期:2007-01-01 00:00:00
abstract::MobA protein, encoded by the broad host-range plasmid R1162, is required for conjugal mobilization of this plasmid. The protein is an essential part of the relaxosome, and is also necessary for the termination of strand transfer. In vitro, MobA is a nuclease specific for one of the two DNA strands of the origin of tra...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.19.4563
更新日期:1993-09-25 00:00:00
abstract::A cDNA clone covering the whole coding region for a glycinin subunit precursor containing the A1a acidic subunit, one of the A2 family, has been identified from a library of soybean cotyledonary cDNA clones using a mixed oligonucleotide probe. Analysis of the cDNA insert revealed that it contained 1746 nucleotides of ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.18.6719
更新日期:1985-09-25 00:00:00
abstract::We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process was mediated by a s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn359
更新日期:2008-08-01 00:00:00
abstract::RNA is ideally suited for in vitro evolution experiments, because a single RNA molecule possesses both genotypic (replicable sequence) and phenotypic (selectable shape) properties. Using systematic evolution of ligands by exponential enrichment (SELEX), we found a single 58-nt aptamer sequence that assumes two structu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp284
更新日期:2009-07-01 00:00:00
abstract::BRENDA (BRaunschweig ENzyme DAtabase) represents a comprehensive collection of enzyme and metabolic information, based on primary literature. The database contains data from at least 83,000 different enzymes from 9800 different organisms, classified in approximately 4200 EC numbers. BRENDA includes biochemical and mol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh081
更新日期:2004-01-01 00:00:00
abstract::We report the latest release (version 3.0) of the CATH protein domain database (http://www.cathdb.info). There has been a 20% increase in the number of structural domains classified in CATH, up to 86 151 domains. Release 3.0 comprises 1110 fold groups and 2147 homologous superfamilies. To cope with the increases in di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl959
更新日期:2007-01-01 00:00:00
abstract::The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.15.3851
更新日期:1992-08-11 00:00:00
abstract::It has been inferred from DNA sequence analyses that in echinoderm mitochondria not only the usual asparagine codons AAU and AAC, but also the usual lysine codon AAA, are translated as asparagine by a single mitochondrial (mt) tRNAAsn with the anticodon GUU. Nucleotide sequencing of starfish mt tRNAAsn revealed that t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.7.1683
更新日期:1999-04-01 00:00:00
abstract::TIGRFAMs is a collection of protein family definitions built to aid in high-throughput annotation of specific protein functions. Each family is based on a hidden Markov model (HMM), where both cutoff scores and membership in the seed alignment are chosen so that the HMMs can classify numerous proteins according to the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1043
更新日期:2007-01-01 00:00:00
abstract::The RNA-dependent RNA polymerases (RdRPs) encoded by RNA viruses represent a unique class of nucleic acid polymerases. RdRPs are essential in virus life cycle due to their central role in viral genome replication/transcription processes. However, their contribution in host adaption has not been well documented. By sol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1250
更新日期:2021-01-06 00:00:00
abstract::MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1085
更新日期:2017-01-25 00:00:00
abstract::The Mouse Genome Database (MGD; http://www.informatics.jax.org) is the primary community model organism database for the laboratory mouse and serves as the source for key biological reference data related to mouse genes, gene functions, phenotypes and disease models with a strong emphasis on the relationship of these ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1211
更新日期:2016-01-04 00:00:00
abstract::Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.13.2885
更新日期:1980-07-11 00:00:00
abstract::Spirochetes of the genus Borrelia include the tick-transmitted causative agents of Lyme disease and relapsing fever. They possess unusual genomes composed mainly of linear replicons terminated by closed DNA hairpin telomeres. Hairpin telomeres present an uninterrupted DNA chain to the replication machinery overcoming ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt832
更新日期:2013-12-01 00:00:00
abstract::A study of the activity of deoxyribonucleotide-substituted analogs of the hammerhead domain of RNA catalysis has led to the design of a 14mer oligomer composed entirely of deoxyribonucleotides that promotes the cleavage of an RNA substrate. Characterization of this reaction with sequence variants and mixed DNA/RNA oli...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.20.4092
更新日期:1995-10-25 00:00:00
abstract::The aminoacylation kinetics of T7 transcripts representing defined regions of Escherichia coli serine tRNAs were determined using purified E.coli seryl-tRNA synthetase (SerRS) and the kinetic values were used to estimate the relative contribution of various tRNA(Ser) domains to recognition by SerRS. The analysis revea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.19.4467
更新日期:1993-09-25 00:00:00
abstract::2'-O-(2-methoxyethyl) (2'-MOE) RNA possesses favorable pharmocokinetic properties that make it a promising option for the design of oligonucleotide drugs. Telomerase is a ribonucleoprotein that is up-regulated in many types of cancer, but its potential as a target for chemotherapy awaits the development of potent and ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.8.1683
更新日期:2001-04-15 00:00:00
abstract::In two-colour microarrays, the ratio of signal intensities of two co-hybridized samples is used as a relative measure of gene expression. Ratio-based analysis becomes complicated and inefficient in multi-class comparisons. We therefore investigated the validity of an intensity-based analysis procedure. To this end, tw...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh038
更新日期:2004-02-24 00:00:00
abstract::An oligodeoxyribonucleotide, d(GCTCACAAT-X-ATTGTGAGC), where X represents a hexaethylene glycol chain, was studied using circular dichroism spectroscopy. Its conformation and conformational stability were compared to those of compounds where X was replaced by four thymines and to the duplex of same sequence without lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.21.6353
更新日期:1990-11-11 00:00:00