Abstract：
:The prokaryotic beta recombinase catalyzes site-specific recombination between two directly oriented minimal six sites in chromatin-integrated substrates. Here, we demonstrate that an enhanced green fluorescent protein (EGFP)-fused version of beta recombinase (beta-EGFP) is fully active, retaining most specific activity. It is used to develop a recombination-dependent activatable gene expression (RAGE) system based on the androgen receptor (AR) ligand-binding domain (LBD). Two hybrid molecules, a direct fusion of the LBD-AR to the C-terminus of beta recombinase (beta-AR) and a triple fusion of beta-EGFP to the same ligand-binding domain (beta-EGFP-AR), were engineered and their subcellular behavior, stability and catalytic activity were evaluated. Both chimeric beta recombinase proteins showed in vivo inducible recombinogenic activity dependent on addition of an androgen receptor agonist, although the beta-AR fusion protein demonstrated more accurate ligand-dependent translocation from cytoplasm to nucleus.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Servert P,Garcia-Castro J,Díaz V,Lucas D,Gonzalez MA,Martínez-A C,Bernad Adoi
10.1093/nar/gnj001keywords:
subject
Has Abstractpub_date
2006-01-03 00:00:00pages
e1issue
1eissn
0305-1048issn
1362-4962pii
34/1/e1journal_volume
34pub_type
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