Nuclear transcriptional activity of the tobacco plastid psbA promoter.

Abstract:

:The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectable phenotype. The transcription start site in the nucleus is site specific and is located 4 nucleotides downstream relative to the start site used in plastids. Translational fusions of the psbA coding sequence with the nptII and bar coding sequences revealed that the psbA leader sequence and the psbA translation start codon, being the second ATG codon, are recognized by the plant cytoplasmic translation apparatus. A promoter cassette utilisable in both E. coli and tobacco, was obtained by placing the CaMV 35S enhancer 5' to the psbA promoter.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Cornelissen M,Vandewiele M

doi

10.1093/nar/17.1.19

subject

Has Abstract

pub_date

1989-01-11 00:00:00

pages

19-29

issue

1

eissn

0305-1048

issn

1362-4962

journal_volume

17

pub_type

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