Abstract:
:The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band separated by PFGE were hybridized with the two independent Notl-linking fragment. Each right and left arms of two Notl-linking fragments were hybridized with one of the two DNA bands separated by PFGE. The results indicated that the chromosomal DNA of S.acidocaldarius is circular.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Yamagishi A,Oshima Tdoi
10.1093/nar/18.5.1133subject
Has Abstractpub_date
1990-03-11 00:00:00pages
1133-6issue
5eissn
0305-1048issn
1362-4962journal_volume
18pub_type
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