当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Abstract：

:Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter PLtetO-1 allows the regulation of gene expression over an up to 5000-fold range with anhydrotetracycline (aTc) whereas with IPTG and arabinose the activity of Plac/ara-1 may be controlled 1800-fold. Escherichia coli host strains which produce defined amounts of the regulatory proteins, Lac and Tet repressor as well as AraC from chromosomally located expression units provide highly reproducible in vivo conditions. Controlling the expression of the genes encoding luciferase, the low abundance E.coli protein DnaJ and restriction endonuclease Cfr9I not only demonstrates that high levels of expression can be achieved but also suggests that under conditions of optimal repression only around one mRNA every 3rd generation is produced. This potential of quantitative control will open up new approaches in the study of gene function in vivo, in particular with low abundance regulatory gene products. The system will also provide new opportunities for the controlled expression of heterologous genes.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Lutz R,Bujard H

doi

10.1093/nar/25.6.1203

subject

Has Abstract

pub_date

1997-03-15 00:00:00

pages

1203-10

issue

6

eissn

0305-1048

issn

1362-4962

pii

gka167

journal_volume

25

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase.

    abstract：:Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence of thioredoxin. The...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkg667

    authors： Davidson JF,Fox R,Harris DD,Lyons-Abbott S,Loeb LA

    更新日期：2003-08-15 00:00:00

  • Inhibition of poly(A) polymerase by rifamycin derivatives.

    abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/1.11.1549

    authors： Jacob ST,Rose KM

    更新日期：1974-11-01 00:00:00

  • High level activity of the mouse CCAAT/enhancer binding protein (C/EBP alpha) gene promoter involves autoregulation and several ubiquitous transcription factors.

    abstract：:The promoter region of the mouse CCAAT-Enhancer Binding Protein (C/EBP alpha) gene is capable of directing high levels of expression of reporter constructs in various cell lines, albeit even in cells that do not express their endogenous C/EBP alpha gene. To understand the molecular mechanisms underlying this ubiquitou...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/21.8.1735

    authors： Legraverend C,Antonson P,Flodby P,Xanthopoulos KG

    更新日期：1993-04-25 00:00:00

  • Distinct functions of the RNA polymerase σ subunit region 3.2 in RNA priming and promoter escape.

    abstract：:The σ subunit of bacterial RNA polymerase (RNAP) has been implicated in all steps of transcription initiation, including promoter recognition and opening, priming of RNA synthesis, abortive initiation and promoter escape. The post-promoter-recognition σ functions were proposed to depend on its conserved region σ3.2 th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1384

    authors： Pupov D,Kuzin I,Bass I,Kulbachinskiy A

    更新日期：2014-04-01 00:00:00

  • A cellular protein binds to a conserved sequence in the adenovirus type 2 enhancer.

    abstract：:A sensitive gel retention assay has been utilized to detect proteins from uninfected Hela nuclei which interact with the adenovirus type 2 enhancer. This assay has been employed to monitor fractionation of nuclear extracts. Three enhancer binding factors were resolved by chromatography on DEAE-Sepharose and one of the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/15.6.2719

    authors： Barrett P,Clark L,Hay RT

    更新日期：1987-03-25 00:00:00

  • Profiling the transcription factor regulatory networks of human cell types.

    abstract：:Neph et al. (2012) (Circuitry and dynamics of human transcription factor regulatory networks. Cell, 150: 1274-1286) reported the transcription factor (TF) regulatory networks of 41 human cell types using the DNaseI footprinting technique. This provides a valuable resource for uncovering regulation principles in differ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku923

    authors： Zhang S,Tian D,Tran NH,Choi KP,Zhang L

    更新日期：2014-11-10 00:00:00

  • Autoregulation of phage mu transposase at the level of translation.

    abstract：:The bacteriophage Mu A and B genes, which lie adjacent to each other and are colinear on the phage genome, encode proteins that catalyze efficient transposition of Mu DNA. We show that the molar ratio of A and B proteins is approximately 1:20 in extracts prepared after induction of cells containing a Mu lysogen or a p...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.23.11285

    authors： Parsons RL,Harshey R

    更新日期：1988-12-09 00:00:00

  • Single molecule detection of PARP1 and PARP2 interaction with DNA strand breaks and their poly(ADP-ribosyl)ation using high-resolution AFM imaging.

    abstract：:PARP1 and PARP2 are implicated in the synthesis of poly(ADP-ribose) (PAR) after detection of DNA damage. The specificity of PARP1 and PARP2 interaction with long DNA fragments containing single- and/or double-strand breaks (SSBs and DSBs) have been studied using atomic force microscopy (AFM) imaging in combination wit...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv1476

    authors： Sukhanova MV,Abrakhi S,Joshi V,Pastre D,Kutuzov MM,Anarbaev RO,Curmi PA,Hamon L,Lavrik OI

    更新日期：2016-04-07 00:00:00

  • The fidelity of template-directed oligonucleotide ligation and its relevance to DNA computation.

    abstract：:Several different computational problems have been solved using DNA as a medium. However, the DNA computations that have so far been carried out have examined a relatively small number of possible sequence solutions in order to find correct sequence solutions. We have encoded a search algorithm in DNA that required th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.22.5203

    authors： James KD,Boles AR,Henckel D,Ellington AD

    更新日期：1998-11-15 00:00:00

  • The missing indels: an estimate of indel variation in a human genome and analysis of factors that impede detection.

    abstract：:With the development of High-Throughput Sequencing (HTS) thousands of human genomes have now been sequenced. Whenever different studies analyze the same genome they usually agree on the amount of single-nucleotide polymorphisms, but differ dramatically on the number of insertion and deletion variants (indels). Further...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv677

    authors： Jiang Y,Turinsky AL,Brudno M

    更新日期：2015-09-03 00:00:00

  • Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.

    abstract：:Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previous preparations of t...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/4.9.3175

    authors： Higgins NP,Geballe AP,Snopek TJ,Sugino A,Cozzarelli NR

    更新日期：1977-09-01 00:00:00

  • ArrayXPath: mapping and visualizing microarray gene-expression data with integrated biological pathway resources using Scalable Vector Graphics.

    abstract：:Biological pathways can provide key information on the organization of biological systems. ArrayXPath (http://www.snubi.org/software/ArrayXPath/) is a web-based service for mapping and visualizing microarray gene-expression data for integrated biological pathway resources using Scalable Vector Graphics (SVG). By integ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkh476

    authors： Chung HJ,Kim M,Park CH,Kim J,Kim JH

    更新日期：2004-07-01 00:00:00

  • Influence of template inactivators on the binding of DNA polymerase to DNA.

    abstract：:The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/1.1.63

    authors： Müller WE,Obermeier J,Totsuka A,Zahn RK

    更新日期：1974-01-01 00:00:00

  • Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags.

    abstract：:In a pilot study on SAGE on Reed-Sternberg cells we have sequenced 1055 tags representing 701 genes. Screening of the GenBank database resulted in the identification of a corresponding gene or EST for 490 of them. For 211 of the tags no homology could be detected. A major problem of the serial analysis of gene express...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.17.e17

    authors： van den Berg A,van der Leij J,Poppema S

    更新日期：1999-09-01 00:00:00

  • Unique organization of the human BCR gene promoter.

    abstract：:The promoter of the human BCR gene, regulating the transcription of the chimeric BCR/ABL mRNA in leukemia, has been isolated and characterized. A region of 1.1 kb immediately 5' to the transcription start site was analyzed in detail by sequencing, DNase 1 footprinting, gel retardation and functional studies. These exp...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.23.7119

    authors： Zhu QS,Heisterkamp N,Groffen J

    更新日期：1990-12-11 00:00:00

  • Interaction of N-terminal domain of U1A protein with an RNA stem/loop.

    abstract：:The U1A protein is a sequence-specific RNA binding protein found in the U1 snRNP particle where it binds to stem/loop II of U1 snRNA. U1A contains two 'RNP' or 'RRM' (RNA Recognition Motif) domains, which are common to many RNA-binding proteins. The N-terminal RRM has been shown to bind specifically to the U1 RNA stem...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.16.4283

    authors： Hall KB,Stump WT

    更新日期：1992-08-25 00:00:00

  • Effect of UV-irradiation on DNA replication of the parvovirus minute-virus-of-mice in mouse fibroblasts.

    abstract：:The effect of UV-irradiation on the conversion of the single-stranded DNA of the parvovirus Minute-Virus-of-Mice (MVM) to duplex Replicative Forms (RF) was studied after infection of mouse A9 fibroblasts. UV-irradiation of the virus prior to infection of unirradiated cells resulted in a dose-dependent, single-hit, inh...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/10.8.2577

    authors： Rommelaere J,Ward DC

    更新日期：1982-04-24 00:00:00

  • Nucleotide sequence and newly formed phosphodiester bond of spontaneously ligated satellite tobacco ringspot virus RNA.

    abstract：:The satellite RNA of tobacco ringspot virus (STobRV RNA) replicates and becomes encapsidated in association with tobacco ringspot virus. Previous results show that the infected tissue produces multimeric STobRV RNAs of both polarities. RNA that is complementary to encapsidated STobRV RNA, designated as having the (-) ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.24.9729

    authors： Buzayan JM,Hampel A,Bruening G

    更新日期：1986-12-22 00:00:00

  • The methylation of one specific guanosine in a pre-tRNA prevents cleavage by RNase P and by the catalytic M1 RNA.

    abstract：:Several modified nucleosides were introduced during in vitro RNA synthesis into a pre-tRNA(Ser). The pre-tRNAs were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP or [alpha-S]-GPT, whereas with m7GTP, the cleavage reaction was completely inhibited. Analysis of pre-tRNAs which contai...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.4.837

    authors： Kahle D,Wehmeyer U,Char S,Krupp G

    更新日期：1990-02-25 00:00:00

  • Structure and expression of an ethylene-related mRNA from tomato.

    abstract：:Messenger RNAs homologous to a cDNA clone (pTOM 13) derived from a ripe-tomato-specific cDNA library are expressed during tomato fruit ripening and after the wounding of leaf and green fruit material. Both responses involve the synthesis of the hormone ethylene. Accumulation of the pTOM 13--homologous RNA during ripen...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/15.2.731

    authors： Holdsworth MJ,Bird CR,Ray J,Schuch W,Grierson D

    更新日期：1987-01-26 00:00:00

  • Highly cost-efficient genome-wide association studies using DNA pools and dense SNP arrays.

    abstract：:Genome-wide association (GWA) studies to map genes for complex traits are powerful yet costly. DNA-pooling strategies have the potential to dramatically reduce the cost of GWA studies. Pooling using Affymetrix arrays has been proposed and used but the efficiency of these arrays has not been quantified. We compared and...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm1060

    authors： Macgregor S,Zhao ZZ,Henders A,Nicholas MG,Montgomery GW,Visscher PM

    更新日期：2008-04-01 00:00:00

  • Functional analysis and identification of cis-regulatory elements of human chromosome 21 gene promoters.

    abstract：:Given the inherent limitations of in silico studies relying solely on DNA sequence analysis, the functional characterization of mammalian promoters and associated cis-regulatory elements requires experimental support, which demands cloning and analysis of putative promoter regions. Focusing on human chromosome 21, we ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq402

    authors： Warnatz HJ,Querfurth R,Guerasimova A,Cheng X,Haas SA,Hufton AL,Manke T,Vanhecke D,Nietfeld W,Vingron M,Janitz M,Lehrach H,Yaspo ML

    更新日期：2010-10-01 00:00:00

  • SIDDBASE: a database containing the stress-induced DNA duplex destabilization (SIDD) profiles of complete microbial genomes.

    abstract：:Prokaryotic genomic DNA is generally negatively supercoiled in vivo. Many regulatory processes, including the initiation of transcription, are known to depend on the superhelical state of the DNA substrate. The stresses induced within DNA by negative superhelicity can destabilize the DNA duplex at specific sites. Vari...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkj007

    authors： Wang H,Kaloper M,Benham CJ

    更新日期：2006-01-01 00:00:00

  • VISIBIOweb: visualization and layout services for BioPAX pathway models.

    abstract：:With recent advancements in techniques for cellular data acquisition, information on cellular processes has been increasing at a dramatic rate. Visualization is critical to analyzing and interpreting complex information; representing cellular processes or pathways is no exception. VISIBIOweb is a free, open-source, we...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq352

    authors： Dilek A,Belviranli ME,Dogrusoz U

    更新日期：2010-07-01 00:00:00

  • Upstream box/TATA box order is the major determinant of the direction of transcription.

    abstract：:Mammalian gene promoters for transcription by RNA polymerase II are typically organized in the following order: upstream sequence motif(s)/TATA box/initiation site. Here we report studies in which the order, orientation and DNA sequences of these three elements are varied to determine how these affect polarity of tran...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.24.6699

    authors： Xu LC,Thali M,Schaffner W

    更新日期：1991-12-25 00:00:00

  • From benchmarking HITS-CLIP peak detection programs to a new method for identification of miRNA-binding sites from Ago2-CLIP data.

    abstract：:Experimental evidence indicates that about 60% of miRNA-binding activity does not follow the canonical rule about the seed matching between miRNA and target mRNAs, but rather a non-canonical miRNA targeting activity outside the seed or with a seed-like motifs. Here, we propose a new unbiased method to identify canonic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx007

    authors： Bottini S,Hamouda-Tekaya N,Tanasa B,Zaragosi LE,Grandjean V,Repetto E,Trabucchi M

    更新日期：2017-05-19 00:00:00

  • The Tudor protein Veneno assembles the ping-pong amplification complex that produces viral piRNAs in Aedes mosquitoes.

    abstract：:PIWI-interacting RNAs (piRNAs) comprise a class of small RNAs best known for suppressing transposable elements in germline tissues. The vector mosquito Aedes aegypti encodes seven PIWI genes, four of which are somatically expressed. This somatic piRNA pathway generates piRNAs from viral RNA during infection with cytop...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky1266

    authors： Joosten J,Miesen P,Taşköprü E,Pennings B,Jansen PWTC,Huynen MA,Vermeulen M,Van Rij RP

    更新日期：2019-03-18 00:00:00

  • A minor class of 5S rRNA genes in Saccharomyces cerevisiae X2180-1B, one member of which lies adjacent to a Ty transposable element.

    abstract：:In Saccharomyces cerevisiae the majority of the genes for 5S rRNA lie within a 9kb rDNA sequence that is present as 100-200 tandemly-repeated copies on Chromosome XII. Following our observations that about 10% of yeast 5S rRNA exists as minor variant sequences, we screened a collection of yeast DNA fragments cloned in...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.10.4083

    authors： Piper PW,Lockheart A,Patel N

    更新日期：1984-05-25 00:00:00

  • Enhanced RNA cleavage within bulge-loops by an artificial ribonuclease.

    abstract：:Cleavage of phosphodiester bonds by small ribonuclease mimics within different bulge-loops of RNA was investigated. Bulge-loops of different size (1-7 nt) and sequence composition were formed in a 3' terminal fragment of influenza virus M2 RNA (96 nt) by hybridization of complementary oligodeoxynucleotides. Small bulg...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gki264

    authors： Kuznetsova IL,Zenkova MA,Gross HJ,Vlassov VV

    更新日期：2005-02-24 00:00:00

  • Complementation of adenovirus E4 mutants by transient expression of E4 cDNA and deletion plasmids.

    abstract：:Human adenovirus mutants that carry a large deletion in early region 4 (E4) are severely defective in the synthesis of viral late proteins. Plasmids that carry intact E4 sequences can complement the late protein synthetic defect of such mutants when introduced into infected cells by transfection, presumably due to the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/17.8.3037

    authors： Ketner G,Bridge E,Virtanen A,Hemström C,Pettersson U

    更新日期：1989-04-25 00:00:00