Abstract:
:A type II restriction endonuclease, DraI, isolated from Deinococcus radiophilus ATCC 27603 recognises the palindromic hexanucleotide sequence (formula; see text) and cleaves it, as indicated by the arrows, to produce blunt-ended fragments. The yield of enzyme is 100 to 1000 times that of the only other known type II restriction endonuclease that recognises a sequence composed solely of A:T basepairs, the isoschizomer AhaIII (1). Ultraviolet irradiation of the DNA substrate at relatively low doses inhibits the activity of DraI by "protecting" the recognition sequence and this may be exploited to give control of partial digestion of DNA by DraI.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Purvis IJ,Moseley BEdoi
10.1093/nar/11.16.5467subject
Has Abstractpub_date
1983-08-25 00:00:00pages
5467-74issue
16eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::The U1A protein is a sequence-specific RNA binding protein found in the U1 snRNP particle where it binds to stem/loop II of U1 snRNA. U1A contains two 'RNP' or 'RRM' (RNA Recognition Motif) domains, which are common to many RNA-binding proteins. The N-terminal RRM has been shown to bind specifically to the U1 RNA stem...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.16.4283
更新日期:1992-08-25 00:00:00
abstract::CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv405
更新日期:2015-05-26 00:00:00
abstract::Herpes simplex virus-1 US11 is a RNA-binding protein with a novel RNA-binding domain. US11 has been reported to exhibit sequence- and conformation-specific RNA-binding, but the sequences and conformations important for binding are not known. US11 has also been described as a double-stranded RNA (dsRNA)-binding protein...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki919
更新日期:2005-10-24 00:00:00
abstract::Genome-wide association (GWA) studies to map genes for complex traits are powerful yet costly. DNA-pooling strategies have the potential to dramatically reduce the cost of GWA studies. Pooling using Affymetrix arrays has been proposed and used but the efficiency of these arrays has not been quantified. We compared and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm1060
更新日期:2008-04-01 00:00:00
abstract::A mutation in a gene dicA of Escherichia coli leads to temperature-sensitive cell division, by allowing expression of a nearby division inhibition gene dicB (1). We have now established the sequence of the DicA region and identified DicA as a 15.5 KD protein. A second gene dicC transcribed divergently from dicA and co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.17.6821
更新日期:1986-09-11 00:00:00
abstract::DNA polymerases of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl group on the incoming nucleotide. In the family Y (lesion bypass) polyme...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg417
更新日期:2003-07-15 00:00:00
abstract::Sequencing of the 3'-untranslated region of the CCT8 gene of the fungal pathogen Candida albicans revealed that the CCT8 coding region overlaps 13 bp with the coding region of the convergently orientated TRP1 gene. The same overlap was found in three strains with different genetic backgrounds. 3'-RACE was used to dete...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.22.5061
更新日期:1998-11-15 00:00:00
abstract::The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp161
更新日期:2009-06-01 00:00:00
abstract::Alterations in cancer genomes strongly influence clinical responses to treatment and in many instances are potent biomarkers for response to drugs. The Genomics of Drug Sensitivity in Cancer (GDSC) database (www.cancerRxgene.org) is the largest public resource for information on drug sensitivity in cancer cells and mo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1111
更新日期:2013-01-01 00:00:00
abstract::Mononegaviruses, such as Ebola virus, encode an L (large) protein that bears all the catalytic activities for replication/transcription and RNA capping. The C-terminal conserved region VI (CRVI) of L protein contains a K-D-K-E catalytic tetrad typical for 2'O methyltransferases (MTase). In mononegaviruses, cap-MTase a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky637
更新日期:2018-09-06 00:00:00
abstract::In non-malignant RWPE-1 prostate epithelial cells signaling by the nuclear receptor Vitamin D Receptor (VDR, NR1I1) induces cell cycle arrest through targets including CDKN1A (encodes p21((waf1/cip1))). VDR dynamically induced individual histone modification patterns at three VDR binding sites (R1, 2, 3) on the CDKN1A...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq875
更新日期:2011-03-01 00:00:00
abstract::We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn222
更新日期:2008-07-01 00:00:00
abstract::We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.10.3375
更新日期:1983-05-25 00:00:00
abstract::DNA methylation plays an important role in many biological processes. Existing epigenome-wide association studies (EWAS) have successfully identified aberrantly methylated genes in many diseases and disorders with most studies focusing on analysing methylation sites one at a time. Incorporating prior biological inform...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw546
更新日期:2016-09-19 00:00:00
abstract::mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Us...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4538
更新日期:1998-10-01 00:00:00
abstract::Hepatitis C virus (HCV) infection is a major cause of human chronic liver disease and hepatocellular carcinoma. G-quadruplex (G4) is an important four-stranded secondary structure of nucleic acids. Recently, we discovered that the core gene of HCV contains a G4 RNA structure; however, the interaction between the HCV c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1177
更新日期:2019-01-10 00:00:00
abstract::The persistence of fetal hemoglobin in many patients with deletion type beta thalassemias and the expression patterns of human globin genes in transgenic mice suggest that gamma- to beta-globin gene switching results primarily from competition of gamma- and beta-globin genes for interaction with the beta-globin locus ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.14.2736
更新日期:2000-07-15 00:00:00
abstract::Details of polynucleotide flexibility may be probed through a combination of semiempirical potential energy calculations and statistical mechanical analyses. The pseudorotational motions of the furanose and the long-range correlated rotations of the chain backbone are described briefly here. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.3.777
更新日期:1982-02-11 00:00:00
abstract::The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.2889
更新日期:1991-06-11 00:00:00
abstract::Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky381
更新日期:2018-07-02 00:00:00
abstract::We show how to speed up sequence alignment algorithms of the type introduced by Needleman and Wunsch (and generalized by Sellers and others). Faster alignment algorithms have been introduced, but always at the cost of possibly getting sub-optimal alignments. Our modification results in the optimal alignment still bein...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.1part1.175
更新日期:1984-01-11 00:00:00
abstract::The Mediator complex transmits activation signals from DNA bound transcription factors to the core transcription machinery. Genome wide localization studies have demonstrated that Mediator occupancy not only correlates with high levels of transcription, but that the complex also is present at transcriptionally silence...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr551
更新日期:2011-10-01 00:00:00
abstract::Synthesis of a small amount of 42S RNA in addition to the VSV specific mRNA species was observed in a coupled transcription-translation system containing ribonucleoprotein particles from L cell infected with vesicular stomatitis virus and nuclease-treated ribosomal extract obtained from uninfected HeLa cells. Analysis...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.20.6341
更新日期:1982-10-25 00:00:00
abstract::We have used DNase I footprinting to examine the association kinetics of GA-, GT- and CT-containing oligonucleotides with the target sequence (GGA)5GG. (CCT)5CC. These reactions are slow yielding bimolecular association rate constants between 50 and 2000 M-1s-1. We find that GT-containing oligonucleotides bind much fa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.16.3269
更新日期:1997-08-15 00:00:00
abstract::The interaction of RNase T1 with calf thymus DNA was studied using uv difference spectroscopy and the effect of the enzyme on DNA melting. There was no indication of RNase T1 binding with native DNA. A prominent difference spectrum for RNase T1 binding with denatured DNA (d-DNA) was observed at pH 5, 25 degrees and lo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.1.11
更新日期:1975-01-01 00:00:00
abstract::The chicken c-myb gene and the v-myb oncogene transduced by avian myeloblastosis virus (AMV) encode DNA binding transcription activators. The DNA binding domain of AMV v-Myb displays a number of amino acid changes relative to c-Myb; v-Myb proteins in which one or more of three crucial residues in the DNA binding domai...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.12.3043
更新日期:1992-06-25 00:00:00
abstract::Translation Initiator of Short 5' UTR (TISU) is a unique regulatory element of both transcription and translation initiation. It is present in a sizable number of genes with basic cellular functions and a very short untranslated region (5' UTR). Here, we investigated translation initiation from short 5' UTR mRNAs with...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr484
更新日期:2011-09-01 00:00:00
abstract::The nuclear suppressor allele NSM3 in strain FF1210-6C/170-E22 (E22), which suppresses a mutation of the yeast mitochondrial tRNA(Asp)gene in Saccharomyces cerevisiae, was cloned and identified. To isolate the NSM3 allele, a genomic DNA library using the vector YEp13 was constructed from strain E22. Nine YEp13 recombi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.7.1542
更新日期:2000-04-01 00:00:00
abstract::Nematode.net (http://nematode.net) is a publicly available resource dedicated to the study of parasitic nematodes. In 2000, the Genome Center at Washington University (GC) joined a consortium including the Nematode Genomics group in Edinburgh, and the Pathogen Sequencing Unit of the Sanger Institute to generate expres...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn744
更新日期:2009-01-01 00:00:00
abstract::The role of Ribonuclease H1 (RNase H1) during primer removal and ligation at the mitochondrial origin of light-strand DNA synthesis (OriL) is a key, yet poorly understood, step in mitochondrial DNA maintenance. Here, we reconstitute the replication cycle of L-strand synthesis in vitro using recombinant mitochondrial p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky708
更新日期:2018-10-12 00:00:00