Synthesis in vitro of full length genomic RNA and assembly of the nucleocapsid of vesicular stomatitis virus in a coupled transcription-translation system.

abstract：:DNA adenine methyltransferase (Dam) is widespread and conserved among the γ-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. ...

journal_title：Nucleic acids research

authors： Horton JR,Zhang X,Blumenthal RM,Cheng X

更新日期：2015-04-30 00:00:00

abstract：:The CATH database of protein domain structures (http://www.biochem.ucl.ac.uk/bsm/cath_new) currently contains 34 287 domain structures classified into 1383 superfamilies and 3285 sequence families. Each structural family is expanded with domain sequence relatives recruited from GenBank using a variety of efficient seq...

journal_title：Nucleic acids research

authors： Pearl FM,Bennett CF,Bray JE,Harrison AP,Martin N,Shepherd A,Sillitoe I,Thornton J,Orengo CA

更新日期：2003-01-01 00:00:00

abstract：:Genetic screens in Saccharomyces cerevisiae have identified the roles of ribosome components, tRNAs and translation factors in translational fidelity. These screens rely on the suppression of altered start codons, nonsense codons or frameshift mutations in genes involved in amino acid or nucleotide metabolism. Many of...

journal_title：Nucleic acids research

authors： Magazinnik T,Anand M,Sattlegger E,Hinnebusch AG,Kinzy TG

更新日期：2005-08-12 00:00:00

abstract：:Reconstructing full-length transcript isoforms from sequence fragments (such as ESTs) is a major interest and challenge for bioinformatic analysis of pre-mRNA alternative splicing. This problem has been formulated as finding traversals across the splice graph, which is a directed acyclic graph (DAG) representation of ...

journal_title：Nucleic acids research

authors： Xing Y,Yu T,Wu YN,Roy M,Kim J,Lee C

更新日期：2006-06-06 00:00:00

abstract：:The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other h...

journal_title：Nucleic acids research

authors： Winkle SA,Rosenberg LS,Krugh TR

更新日期：1982-12-20 00:00:00

abstract：:A specific segment of mitochondrial DNA from 18 people was examined by two methods of direct DNA sequencing. This segment includes a small noncoding region (V) shown before by restriction analysis to exhibit length polymorphism. All 11 of the human mtDNAs previously reported to have a deletion in this region proved to...

journal_title：Nucleic acids research

authors： Wrischnik LA,Higuchi RG,Stoneking M,Erlich HA,Arnheim N,Wilson AC

更新日期：1987-01-26 00:00:00

abstract：:The PathoSystems Resource Integration Center (PATRIC) is the bacterial Bioinformatics Resource Center funded by the National Institute of Allergy and Infectious Diseases (https://www.patricbrc.org). PATRIC supports bioinformatic analyses of all bacteria with a special emphasis on pathogens, offering a rich comparative...

journal_title：Nucleic acids research

authors： Davis JJ,Wattam AR,Aziz RK,Brettin T,Butler R,Butler RM,Chlenski P,Conrad N,Dickerman A,Dietrich EM,Gabbard JL,Gerdes S,Guard A,Kenyon RW,Machi D,Mao C,Murphy-Olson D,Nguyen M,Nordberg EK,Olsen GJ,Olson RD,Overb

更新日期：2020-01-08 00:00:00

abstract：:The study of pathogenic mitochondrial DNA mutations has, in most cases, relied on the production of transmitochondrial cybrids. Although the procedure to produce such cybrids is well established, it is laborious and cumbersome. Moreover, the mechanical enucleation procedure is inefficient and different techniques have...

journal_title：Nucleic acids research

authors： Bayona-Bafaluy MP,Manfredi G,Moraes CT

更新日期：2003-08-15 00:00:00

abstract：:The G- and U-specific reagent, carbodiimide was used to probe the solution structure of aminoacylated yeast phenylalanine tRNA. Both quantitative and qualitative changes in modification were observed when the modification patterns of tRNA-CCA(3'OH), tRNA-CCA(3'NH2) and phe-tRNA-CCA(3'NH2) were compared. Five nucleotid...

journal_title：Nucleic acids research

authors： Fritzinger DC,Fournier MJ

更新日期：1982-04-10 00:00:00

abstract：:We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist t...

journal_title：Nucleic acids research

authors： Mytelka DS,Chamberlin MJ

更新日期：1996-07-15 00:00:00

abstract：:Microsatellites are widely used as genetic markers because they are co-dominant, multiallelic, easily scored and highly polymorphic. A major drawback of microsatellite markers is the time and cost required to characterise them. We have developed a novel technique to reduce this cost by producing a microsatellite-rich ...

journal_title：Nucleic acids research

authors： Fisher PJ,Gardner RC,Richardson TE

更新日期：1996-11-01 00:00:00

abstract：:The UCSC Genome Browser (https://genome.ucsc.edu) provides a web interface for exploring annotated genome assemblies. The assemblies and annotation tracks are updated on an ongoing basis-12 assemblies and more than 28 tracks were added in the past year. Two recent additions are a display of CRISPR/Cas9 guide sequences...

journal_title：Nucleic acids research

authors： Casper J,Zweig AS,Villarreal C,Tyner C,Speir ML,Rosenbloom KR,Raney BJ,Lee CM,Lee BT,Karolchik D,Hinrichs AS,Haeussler M,Guruvadoo L,Navarro Gonzalez J,Gibson D,Fiddes IT,Eisenhart C,Diekhans M,Clawson H,Barber GP,

更新日期：2018-01-04 00:00:00

abstract：:A downstream G-rich sequence (GRS), GGGGGAGGUGUGGG, has been previously shown to influence the efficiency of 3' end processing of the SV40 late polyadenylation signal. We have now defined several important parameters for GRS-mediated polyadenylation. The ability of the GRS to influence 3' end processing efficiency was...

journal_title：Nucleic acids research

authors： Bagga PS,Ford LP,Chen F,Wilusz J

更新日期：1995-05-11 00:00:00

abstract：:For the analysis of protein-DNA interactions by coupled gel-shift/footprinting, DNA fragments need to be extracted from polyacrylamide gels and subsequently separated on high resolution gels. Due to impurities in the extracted DNA, single nucleotide resolution is frequently not achieved. We now describe an improved ex...

journal_title：Nucleic acids research

authors： Ragnhildstveit E,Fjose A,Becker PB,Quivy JP

更新日期：1997-01-15 00:00:00

abstract：:The interaction of antibodies directed toward N6-(delta2-isopentenyl)adenosine, i6Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoassay to quantitate th...

journal_title：Nucleic acids research

authors： Milstone DS,Vold BS,Glitz DG,Shutt N

更新日期：1978-09-01 00:00:00

abstract：:The non-essential MGS1 gene of Saccharomyces cerevisiae is highly conserved in eukaryotes and encodes an enzyme containing both DNA-dependent ATPase and DNA annealing activities. MGS1 appears to function in post-replicational repair processes that contribute to genome stability. In this study, we identified MGS1 as a ...

journal_title：Nucleic acids research

authors： Kim JH,Kang YH,Kang HJ,Kim DH,Ryu GH,Kang MJ,Seo YS

更新日期：2005-10-26 00:00:00

abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...

journal_title：Nucleic acids research

authors： Lu Q,Schierer T,Kang SG,Henderson E

更新日期：1998-04-01 00:00:00

abstract：:We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Gen...

journal_title：Nucleic acids research

authors： Strauss EJ,Guthrie C

更新日期：1994-08-11 00:00:00

abstract：:MiRNAs are important regulators of gene expression and are frequently deregulated under pathologic conditions. They are highly stable in bodily fluids which makes them feasible candidates to become minimally invasive biomarkers. In fact, several studies already proposed circulating miRNA-based biomarkers for different...

journal_title：Nucleic acids research

authors： Aparicio-Puerta E,Jáspez D,Lebrón R,Koppers-Lalic D,Marchal JA,Hackenberg M

更新日期：2019-01-08 00:00:00

abstract：:The paper describes an improved method for the synthesis of oligodeoxyribonucleotides using phosphoramidite chemistry. Our procedure relies on novel phosphoramidite intermediates, the deoxyribonucleoside-3'-morpholine-methoxyphosphins. These compounds are extremely stable and can be purified readily. Condensation reac...

journal_title：Nucleic acids research

authors： Dörper T,Winnacker EL

更新日期：1983-05-11 00:00:00

abstract：:Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage an...

journal_title：Nucleic acids research

authors： Woodcock DM,Crowther PJ,Doherty J,Jefferson S,DeCruz E,Noyer-Weidner M,Smith SS,Michael MZ,Graham MW

更新日期：1989-05-11 00:00:00

abstract：:Secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus RNA 3. The structure consists of two stem-loops separated by a single-stranded region. RNA 3-derived transcripts were ...

journal_title：Nucleic acids research

authors： Lauber E,Guilley H,Richards K,Jonard G,Gilmer D

更新日期：1997-12-01 00:00:00

abstract：:The CDC16 gene is involved in the process of chromosome segregation in mitosis and a cdc16ts mutant accumulates the predominant microtubule-associated protein at the nonpermissive temperature. We find that the CDC16 gene open reading frame (ORF) is capable of encoding a protein whose calculated molecular weight and pI...

journal_title：Nucleic acids research

authors： Icho T,Wickner RB

更新日期：1987-10-26 00:00:00

abstract：:Multiple sequence alignments play a central role in the annotation of novel genomes. Given the biological and computational complexity of this task, the automatic generation of high-quality alignments remains challenging. Since multiple alignments are usually employed at the very start of data analysis pipelines, it i...

journal_title：Nucleic acids research

authors： Lassmann T,Sonnhammer EL

更新日期：2005-12-16 00:00:00

abstract：:MolProbity is a general-purpose web server offering quality validation for 3D structures of proteins, nucleic acids and complexes. It provides detailed all-atom contact analysis of any steric problems within the molecules as well as updated dihedral-angle diagnostics, and it can calculate and display the H-bond and va...

journal_title：Nucleic acids research

authors： Davis IW,Leaver-Fay A,Chen VB,Block JN,Kapral GJ,Wang X,Murray LW,Arendall WB 3rd,Snoeyink J,Richardson JS,Richardson DC

更新日期：2007-07-01 00:00:00

abstract：:We have studied the effect of selenium on the expression of a cellular glutathione peroxidase, GSHPx-1, in transfected MCF-7 cells and in doxorubicin-resistant (Adrr) MCF-7 cells. A GSHPx-1 cDNA with a Rous Sarcoma virus promoter was transfected into a human mammary carcinoma cell line, MCF-7, which has very low endog...

journal_title：Nucleic acids research

authors： Chu FF,Esworthy RS,Akman S,Doroshow JH

更新日期：1990-03-25 00:00:00

abstract：:Human cytomegalovirus (HCMV) terminase is composed of subunits pUL56 (130 kDa) and pUL89 ( approximately 75 kDa), encoded by the UL56 and UL89 genes. In a recent investigation, we demonstrated that the main ATPase activity is associated with the large terminase subunit pUL56. The protein has two putative ATP-binding s...

journal_title：Nucleic acids research

authors： Scholz B,Rechter S,Drach JC,Townsend LB,Bogner E

更新日期：2003-03-01 00:00:00

abstract：:The interactions of archetypical nucleic acid ligands with the HIV-1 polypurine tract (PPT) RNA:DNA hybrid, as well as analogous DNA:DNA, RNA:RNA and swapped hybrid substrates, were used to probe structural features of the PPT that contribute to its specific recognition and processing by reverse transcriptase (RT). Re...

journal_title：Nucleic acids research

authors： Turner KB,Brinson RG,Yi-Brunozzi HY,Rausch JW,Miller JT,Le Grice SF,Marino JP,Fabris D

更新日期：2008-05-01 00:00:00

abstract：:Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...

journal_title：Nucleic acids research

authors： Meades G Jr,Benson BK,Grove A,Waldrop GL

更新日期：2010-03-01 00:00:00

abstract：:Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma. The plasmid, named L factor, was found at a high copy number (5,000 or more per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA co...

journal_title：Nucleic acids research

authors： Saito H,Uehara H,Kusano T,Oishi M

更新日期：1987-11-11 00:00:00