Abstract：
:In this paper, we present evidence that an efficient Rho-dependent terminator can be created by introducing a simple (AG/TC) n DNA repeat into a transcription unit. The Rho termination activity in vivo and in vitro is dependent on the length and the orientation of the insert. The transcription of at least 30 bp of the (AG/TC) n repeat in the orientation encoding the (rUrC) n sequence on the transcript leads to Rho-dependent termination at a downstream non-terminator site. Our results indicate that the high efficiency of this artificial Rho-dependent terminator is due to optimal interactions between the (rUrC) n RNA sequence and Rho protein. Thus, our findings strongly suggest that an adequate loading site is the primary determinant for Rho termination activity and provide a more defined system for future investigations.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Guérin M,Robichon N,Geiselmann J,Rahmouni ARdoi
10.1093/nar/26.21.4895subject
Has Abstractpub_date
1998-11-01 00:00:00pages
4895-900issue
21eissn
0305-1048issn
1362-4962pii
gkb785journal_volume
26pub_type
杂志文章abstract：:Protein-protein and protein-DNA/RNA interactions play a fundamental role in a variety of biological processes. Determining the complex structures of these interactions is valuable, in which molecular docking has played an important role. To automatically make use of the binding information from the PDB in docking, her...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx407
更新日期：2017-07-03 00:00:00
abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni143
更新日期：2005-10-06 00:00:00
abstract：:Stringent starvation protein A (SspA) is an RNA polymerase (RNAP)-associated protein involved in nucleotide metabolism, acid tolerance and virulence of bacteria. Despite extensive biochemical and genetic analyses, the precise regulatory role of SspA in transcription is still unknown, in part, because of a lack of stru...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa672
更新日期：2020-09-25 00:00:00
abstract：:The Bovine Genome Database (BGD) (http://bovinegenome.org) has been the key community bovine genomics database for more than a decade. To accommodate the increasing amount and complexity of bovine genomics data, BGD continues to advance its practices in data acquisition, curation, integration and efficient data retrie...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz944
更新日期：2020-01-08 00:00:00
abstract：:A fluoride-cleavable phosphoramidite for biotinylation was designed, synthesized and coupled efficiently to the 5'-end of DNA on an automatic synthesizer. The diisopropylsilyl acetal functionality was used to link the biotin moiety through a tertiary hydroxide group to the 5'-end of DNA. This linkage proved to be comp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg130
更新日期：2003-01-15 00:00:00
abstract：:Homozygosity for a natural deletion variant of the HIV-coreceptor molecule CCR5, CCR5Δ32, confers resistance toward HIV infection. Allogeneic stem cell transplantation from a CCR5Δ32-homozygous donor has resulted in the first cure from HIV ('Berlin patient'). Based thereon, genetic disruption of CCR5 using designer nu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv469
更新日期：2015-06-23 00:00:00
abstract：:Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed in several types of cancer. However, the mechanisms that mediate their function and the composition of the variant-containing chromatin are still largely unknown. A proteomic interrogation of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku303
更新日期：2014-06-01 00:00:00
abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.14.5651
更新日期：1986-07-25 00:00:00
abstract：:The genetic organization of the multiple ribosomal transcription units (RTUs) on the genome of the yeast Saccharomyces carlsbergensis was studied by electron microscopy of purified ribosomal DNA hybridized to 26S rRNA using the R-loop technique (Thomas, M., White, R.L. and Davis, R.W. (1973) Proc. Natl. Acad. Sci. U.S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.8.2801
更新日期：1978-08-01 00:00:00
abstract：:Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previous preparations of t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.9.3175
更新日期：1977-09-01 00:00:00
abstract：:Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm195
更新日期：2007-01-01 00:00:00
abstract：:The majority of genome-wide association study (GWAS) risk variants reside in non-coding DNA sequences. Understanding how these sequence modifications lead to transcriptional alterations and cell-to-cell variability can help unraveling genotype-phenotype relationships. Here, we describe a computational method, dubbed C...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1263
更新日期：2017-03-17 00:00:00
abstract：:Mammalian antibody switch regions (∼1500 bp) are composed of a series of closely neighboring G4-capable sequences. Whereas numerous structural and genome-wide analyses of roles for minimal G4s in transcriptional regulation have been reported, Long G4-capable regions (LG4s)-like those at antibody switch regions-remain ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa357
更新日期：2020-06-19 00:00:00
abstract：:Although the thermophilic bacterium Thermus aquaticus grows optimally at 70 degrees C and cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degrees C. This activity is a bane to some PCRs, since it catalyzes non-specific priming. We report mutations of Klentaq (an N-terminal ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg813
更新日期：2003-11-01 00:00:00
abstract：:The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing e...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng045
更新日期：2003-04-15 00:00:00
abstract：:Transcription of RNA from the beta-globin (major) gene in nuclei from induced mouse erythroleukemia cells terminates within the region between 700 to 2000 bases downstream from the poly(A) addition site, but not at particularly favored sites. In addition we present the first analysis of in vivo labeled RNA from a cell...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.22.8723
更新日期：1984-11-26 00:00:00
abstract：:Affymetrix has recently developed whole-transcript GeneChips-'Gene' and 'Exon' arrays-which interrogate exons along the length of each gene. Although each probe on these arrays is intended to hybridize perfectly to only one transcriptional target, many probes match multiple transcripts located in different parts of th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp853
更新日期：2010-01-01 00:00:00
abstract：:Assessment of the fidelity of gene expression is crucial to understand cell homeostasis. Here we present a highly sensitive method for the systematic Quantification of Rare Amino acid Substitutions (QRAS) using absolute quantification by targeted mass spectrometry after chromatographic enrichment of peptides with miss...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1319
更新日期：2019-04-08 00:00:00
abstract：:Translesion DNA synthesis (TLS) by the Y-family DNA polymerases Polη, Polι and Polκ, mediated via interaction with proliferating cell nuclear antigen (PCNA), is a crucial pathway that protects human cells against DNA damage. We report that Polη has three PCNA-interacting protein (PIP) boxes (PIP1, 2, 3) that contribut...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv712
更新日期：2015-09-18 00:00:00
abstract：:2'-O-Aminooxymethyl ribonucleosides are prepared from their 3',5'-disilylated 2'-O-phthalimidooxymethyl derivatives by treatment with NH(4)F in MeOH. The reaction of these novel ribonucleosides with 1-pyrenecarboxaldehyde results in the efficient formation of stable and yet reversible ribonucleoside 2'-conjugates in y...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr896
更新日期：2012-03-01 00:00:00
abstract：:We report here spectroscopic and biochemical data of a novel series of sugar-modified oligodeoxy-nucleotides, the carbocyclic oligothymidylates, c(dT)3-20. In c(dT)n a methylene group has been substituted for the oxygen atom of the deoxyribose ring of the natural thymidylate unit. c(dT)10-20 form helical structures, i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.8.2133
更新日期：1990-04-25 00:00:00
abstract：:Antibodies to the deoxyribotrinucleotides dpApTpA and dpApApT were prepared by injecting the bovine serum albumin conjugates of the respective haptens in rabbits. The specificities of the antibodies were determined by estimating the inhibition of the binding of the tritiated haptens to the immunoglobulins by various n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.9.3007
更新日期：1977-09-01 00:00:00
abstract：:Quantitative real-time PCR has revolutionized many aspects of genetic research, biomedical diagnostics and pathogen detection. Nevertheless, the full potential of this technology has yet to be realized, primarily due to the limitations of the threshold-based methodologies that are currently used for quantitative analy...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh177
更新日期：2004-12-15 00:00:00
abstract：:The APOBEC3 proteins are unique to mammals. Many inhibit retrovirus infection through a cDNA cytosine deamination mechanism. HIV-1 neutralizes this host defense through Vif, which triggers APOBEC3 ubiquitination and degradation. Here, we report an APOBEC3F-like, double deaminase domain protein from three artiodactyls:...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl721
更新日期：2006-01-01 00:00:00
abstract：:Lysinomicin, a naturally-occurring pseudodisaccharide, inhibits translation in prokaryotes. We report that lysinomicin (and three related compounds) are able to inhibit the self-splicing of group I introns, thus identifying pseudodisaccharides as a novel class of group I intron splicing inhibitors. Lysinomicin inhibit...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.23.4983
更新日期：1994-11-25 00:00:00
abstract：:The active 3D conformation of the spliceosome's catalytic U2/U6 RNA core is stabilised by a network of secondary and tertiary RNA interactions, but also depends on spliceosomal proteins for its formation. To determine the contribution towards splicing of specific RNA secondary and tertiary interactions in the U2/U6 RN...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky966
更新日期：2018-12-14 00:00:00
abstract：:Human tissue-specific genes were reported to be longer than housekeeping genes (both in coding and intronic parts). The competing neutralist and adaptationist models were proposed to explain this observation. Here I show that in human genome the longest are genes with the intermediate expression pattern. From the stan...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl773
更新日期：2006-01-01 00:00:00
abstract：:We have determined the sequence of 80 nucleotides adjacent to 3' poly(A) of both (middle and bottom component) cowpea mosaic virus RNAs, using dideoxynucleotide termination of reverse transcription. Sequence conservation is indicated, there being about 80% homology between the first 65 bases of each RNA. Although both...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.2.493
更新日期：1979-09-25 00:00:00
abstract：:Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct complexes with 5S ribosomal RNA in Xenopus oocytes. Additionally, TFIIIA binds the internal promoter of the 5S RNA gene and supports assembly of a transcription initiation complex. Both proteins have nine tandemly repeated zinc fingers with a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.3.703
更新日期：1998-02-01 00:00:00
abstract：:The Aspergillus Genome Database (AspGD) is an online genomics resource for researchers studying the genetics and molecular biology of the Aspergilli. AspGD combines high-quality manual curation of the experimental scientific literature examining the genetics and molecular biology of Aspergilli, cutting-edge comparativ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp751
更新日期：2010-01-01 00:00:00
