Conformation of DNA in chromatin reconstituted from poly [d(A-T)] and the core histones.

abstract：:Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the...

journal_title：Nucleic acids research

authors： Chasteen L,Ayriss J,Pavlik P,Bradbury AR

更新日期：2006-01-01 00:00:00

abstract：:The nucleoside analogs 1-(2'-deoxy-beta-D-ribofuranosyl)- 3-nitropyrrole (9), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitropyrazole (10), 1-(2'-deoxy-beta-D-ribofuranosyl)-4-nitroimidazole (11) and 1-(2'-deoxy-beta-D-ribofuranosyl)-5-nitroindole (21) were incorporated into the oligonucleotide 5'-d(CGCXAATTYGCG)-3'in the f...

journal_title：Nucleic acids research

authors： Bergstrom DE,Zhang P,Johnson WT

更新日期：1997-05-15 00:00:00

abstract：:Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected mola...

journal_title：Nucleic acids research

authors： Gegenheimer P

更新日期：1988-03-11 00:00:00

abstract：:Molecular replacement (MR) is the method of choice for X-ray crystallography structure determination when structural homologues are available in the Protein Data Bank (PDB). Although the success rate of MR decreases sharply when the sequence similarity between template and target proteins drops below 35% identical res...

journal_title：Nucleic acids research

authors： Claude JB,Suhre K,Notredame C,Claverie JM,Abergel C

更新日期：2004-07-01 00:00:00

abstract：:We show that the cAMP receptor protein (Crp) binds to DNA as several different conformers. This situation has precluded discovering a high correlation between any sequence property and binding affinity for proteins that bend DNA. Experimentally quantified affinities of Synechocystis sp. PCC 6803 cAMP receptor protein ...

journal_title：Nucleic acids research

authors： Holmquist PC,Holmquist GP,Summers ML

更新日期：2011-08-01 00:00:00

abstract：:A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction...

journal_title：Nucleic acids research

authors： Watkins PC,Tanzi RE,Gibbons KT,Tricoli JV,Landes G,Eddy R,Shows TB,Gusella JF

更新日期：1985-09-11 00:00:00

abstract：:Genomic DNA methylation contributes substantively to transcriptional regulations that underlie mammalian development and cellular differentiation. Much effort has been made to decipher the molecular mechanisms governing the establishment and maintenance of DNA methylation patterns. However, little is known about genom...

journal_title：Nucleic acids research

authors： Xie H,Wang M,de Andrade A,Bonaldo Mde F,Galat V,Arndt K,Rajaram V,Goldman S,Tomita T,Soares MB

更新日期：2011-05-01 00:00:00

abstract：:Using synthetic oligodeoxyribonucleotides to prime the transcription of interferon mRNA and cDNA, we recently determined the mRNA sequence coding for the 47 amino-terminal amino acids of mature human fibroblast interferon (1). From this sequence, we have now synthesised an oligodeoxyribonucleotide that is homologous w...

journal_title：Nucleic acids research

authors： Houghton M,Easton MA,Stewart AG,Smith JC,Doel SM,Catlin GH,Lewis HM,Patel TP,Emtage JS,Carey NH,Porter AG

更新日期：1980-07-11 00:00:00

abstract：:Oligodeoxyribonucleotides containing N4-methoxycytosine (mo4C), N4-methoxy-5-methylcytosine (mo4m5C) and other base-analogues were synthesised and used to compare the stabilities of duplexes containing mo4C.A and mo4C.G base pairs with those containing normal and mismatch pairs. The Tm values and other thermodynamic p...

journal_title：Nucleic acids research

authors： Anand NN,Brown DM,Salisbury SA

更新日期：1987-10-26 00:00:00

abstract：:We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces cerevisiae. Analyses were performed in wild-type as well as mig1 and tup1 mutant strains after the promoter mutants were reintroduced into the native SUC2 locus on the left arm of chromosome IX. Mutation of the two GC boxes reveal...

journal_title：Nucleic acids research

authors： Bu Y,Schmidt MC

更新日期：1998-02-15 00:00:00

abstract：:Homo-oligomerization of proteins is abundant in nature, and is often intimately related with the physiological functions of proteins, such as in metabolism, signal transduction or immunity. Information on the homo-oligomer structure is therefore important to obtain a molecular-level understanding of protein functions ...

journal_title：Nucleic acids research

authors： Baek M,Park T,Heo L,Park C,Seok C

更新日期：2017-07-03 00:00:00

abstract：:To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluorophore. These dUTP derivatives and commercial Cy3- and Cy5-dUTP were stud...

journal_title：Nucleic acids research

authors： Zasedateleva OA,Vasiliskov VA,Surzhikov SA,Kuznetsova VE,Shershov VE,Guseinov TO,Smirnov IP,Yurasov RA,Spitsyn MA,Chudinov AV

更新日期：2018-07-06 00:00:00

abstract：:Codon usage bias is a universal feature of all genomes. Although codon usage has been shown to regulate mRNA and protein levels by influencing mRNA decay and transcription in eukaryotes, little or no genome-wide correlations between codon usage and mRNA levels are detected in mammalian cells, raising doubt on the sign...

journal_title：Nucleic acids research

authors： Yang Q,Lyu X,Zhao F,Liu Y

更新日期：2021-01-25 00:00:00

abstract：:A theoretical approach for linkage mapping the genome of any higher eukaryote is described. It uses the polymerase chain reaction, oligonucleotides of random sequence and single haploid cells. Markers are defined and then the DNA of a single sperm is broken at random (eg by gamma-rays) and physically split into 3 aliq...

journal_title：Nucleic acids research

authors： Dear PH,Cook PR

更新日期：1989-09-12 00:00:00

abstract：:N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1979-02-01 00:00:00

abstract：:DNA molecules with covalently sealed (hairpin) ends are probable intermediates in V(D)J recombination. According to current models hairpin ends are opened to produce short single-stranded extensions that are thought to be precursors of a particular type of extra nucleotides, termed P nucleotides, which are frequently ...

journal_title：Nucleic acids research

authors： Kabotyanski EB,Zhu C,Kallick DA,Roth DB

更新日期：1995-10-11 00:00:00

abstract：:Conventional approaches to predict transcriptional regulatory interactions usually rely on the definition of a shared motif sequence on the target genes of a transcription factor (TF). These efforts have been frustrated by the limited availability and accuracy of TF binding site motifs, usually represented as position...

journal_title：Nucleic acids research

authors： Elati M,Nicolle R,Junier I,Fernández D,Fekih R,Font J,Képès F

更新日期：2013-02-01 00:00:00

abstract：:It has been proposed that DNA multiply damaged sites (MDS), where more than one moiety in a local region ( approximately 1 helical turn, 10 bp) of the DNA is damaged, are lesions of enhanced biological significance. However, other than indirect measures, there are few analytical techniques that allow direct detection ...

journal_title：Nucleic acids research

authors： Bowman KJ,Pla RL,Guichard Y,Farmer PB,Jones GD

更新日期：2001-10-15 00:00:00

abstract：:The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...

journal_title：Nucleic acids research

authors： Burland V,Plunkett G 3rd,Sofia HJ,Daniels DL,Blattner FR

更新日期：1995-06-25 00:00:00

abstract：:The DNA unwinding element (DUE) is a sequence rich in adenine and thymine residues present within the origin region of both prokaryotic and eukaryotic replicons. Recently, it has been shown that this is the site where bacterial DnaA proteins, the chromosomal replication initiators, form a specific nucleoprotein filame...

journal_title：Nucleic acids research

authors： Wegrzyn K,Fuentes-Perez ME,Bury K,Rajewska M,Moreno-Herrero F,Konieczny I

更新日期：2014-07-01 00:00:00

abstract：:DNA methylation plays a crucial role in the establishment of tissue-specific gene expression and the regulation of key biological processes. However, our present inability to predict the effect of genome sequence variation on DNA methylation precludes a comprehensive assessment of the consequences of non-coding variat...

journal_title：Nucleic acids research

authors： Zeng H,Gifford DK

更新日期：2017-06-20 00:00:00

abstract：:The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been proposed as regulators, but a potential role of the nuclear ...

journal_title：Nucleic acids research

authors： Heinz KS,Casas-Delucchi CS,Török T,Cmarko D,Rapp A,Raska I,Cardoso MC

更新日期：2018-07-06 00:00:00

abstract：:Topoisomerases are ubiquitous proteins found in all three domains of life. They change the topology of DNA via transient breaks on either one or two of the DNA strands to allow passage of another single or double DNA strand through the break. Topoisomerases are classified into two types: type I enzymes cleave one DNA ...

journal_title：Nucleic acids research

authors： Baker NM,Rajan R,Mondragón A

更新日期：2009-02-01 00:00:00

abstract：:To investigate the mechanisms by which oligonucleotides hybridize to target molecules, the binding of two oligodeoxynucleotide probes to RNA targets was measured over a broad range of temperatures. Mutations were then scanned across each DNA/RNA hybrid to map, at single base resolution, sequences important for hybridi...

journal_title：Nucleic acids research

authors： Majlessi M,Becker MM

更新日期：2008-05-01 00:00:00

abstract：:An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with activities toward adenine paired with guanine and 8-oxo-7,8-dihydroguanine (8-oxoG) as a single peak corresponding to a 55...

journal_title：Nucleic acids research

authors： Ohtsubo T,Nishioka K,Imaiso Y,Iwai S,Shimokawa H,Oda H,Fujiwara T,Nakabeppu Y

更新日期：2000-03-15 00:00:00

abstract：:HT1080 cells stably expressing green fluorescent protein (GFP) linked to a 3' terminal AU-rich element (ARE) proved to be a convenient system to study the dynamics of mRNA stability, as changes in mRNA levels are reflected in increased or decreased fluorescence intensity. This study examined whether mRNA stability can...

journal_title：Nucleic acids research

authors： Raineri I,Wegmueller D,Gross B,Certa U,Moroni C

更新日期：2004-02-19 00:00:00

abstract：:The bHLH transcription factor MyoD, the prototypical master regulator of differentiation, directs a complex program of gene expression during skeletal myogenesis. The up-regulation of the cdk inhibitor p57kip2 plays a critical role in coordinating differentiation and growth arrest during muscle development, as well as...

journal_title：Nucleic acids research

authors： Busanello A,Battistelli C,Carbone M,Mostocotto C,Maione R

更新日期：2012-09-01 00:00:00

abstract：:The ASTRAL compendium provides several databases and tools to aid in the analysis of protein structures, particularly through the use of their sequences. It is partially derived from the SCOP database of protein domains, and it includes sequences for each domain as well as other resources useful for studying these seq...

journal_title：Nucleic acids research

authors： Chandonia JM,Walker NS,Lo Conte L,Koehl P,Levitt M,Brenner SE

更新日期：2002-01-01 00:00:00

abstract：:The CDC16 gene is involved in the process of chromosome segregation in mitosis and a cdc16ts mutant accumulates the predominant microtubule-associated protein at the nonpermissive temperature. We find that the CDC16 gene open reading frame (ORF) is capable of encoding a protein whose calculated molecular weight and pI...

journal_title：Nucleic acids research

authors： Icho T,Wickner RB

更新日期：1987-10-26 00:00:00

abstract：:A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with its cognate and non cognate yeast tRNAs. It was shown that, when limited amounts of tRNA (either cognate or non cognate) a...

journal_title：Nucleic acids research

authors： Giegé R,Jacrot B,Moras D,Thierry JC,Zaccai G

更新日期：1977-07-01 00:00:00