Abstract：
:We analyzed the effects of site-directed mutations in the SUC2 promoter of Saccharomyces cerevisiae. Analyses were performed in wild-type as well as mig1 and tup1 mutant strains after the promoter mutants were reintroduced into the native SUC2 locus on the left arm of chromosome IX. Mutation of the two GC boxes revealed that these elements play two distinct roles: they are, as expected, required for Mig1-mediated repression but they are also necessary for activation of the SUC2 promoter in response to glucose limitation. The individual GC boxes are functionally redundant with regard to Mig1-mediated repression, however, only the upstream GC box is essential for high level expression of SUC2. Microccocal nuclease sensitivity of the SUC2 promoter in derepressed cells was reduced in the GC box mutant promoters, particularly in the vicinity of the TATA box. The difference in nuclease sensitivity between wild-type and GC box mutant promoters was not evident in tup1- cells. The formation of nuclease-resistant chromatin does not require the GC boxes, indicating that other cis-acting elements can serve to recruit the Ssn6-Tup1 co-repressor complex to the SUC2 promoter.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bu Y,Schmidt MCdoi
10.1093/nar/26.4.1002subject
Has Abstractpub_date
1998-02-15 00:00:00pages
1002-9issue
4eissn
0305-1048issn
1362-4962pii
gkb174journal_volume
26pub_type
杂志文章abstract：:In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.17.3433
更新日期：1997-09-01 00:00:00
abstract：:Minichromosome maintenance (MCM) proteins facilitate replication by licensing origins and unwinding the DNA double strand. Interestingly, the number of MCM hexamers greatly exceeds the number of firing origins suggesting additional roles of MCMs. Here we show a hitherto unanticipated function of MCM2 in cilia formatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky945
更新日期：2019-01-10 00:00:00
abstract：:A single base change in 16S rRNA (C726 to G) has previously been shown to have a dramatic effect on protein synthesis in E. coli (1). This paper more specifically details the effects of the mutation on mRNA binding and translation-termination. The in vitro technique of toeprinting (2) was used to demonstrate that 30S ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.18.5381
更新日期：1990-09-25 00:00:00
abstract：:Initiation of reverse transcription is a complex and regulated process in all retroviruses. Several base pairing interactions have been proposed to occur between the HIV-1 RNA genome and the specific tRNA(lys3) primer. The tRNA primer can form up to 21 bp with the primer binding site (PBS), and an additional 8 bp inte...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg714
更新日期：2003-09-01 00:00:00
abstract：:Oligoamines (spermidine, dipropylenetriamine and propylenediamine) were covalently attached to acridine via a hexamethylene linker. These oligoamine-acridine conjugates were efficiently bound to gap sites in substrate DNA, and promoted the DNA hydrolysis by a homogeneous Ce(IV)/ethylenediamine-N,N,N',N'-tetraacetate (...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg634
更新日期：2003-08-01 00:00:00
abstract：:We describe a cell-free chromatin assembly system derived from the yeast Saccharomyces cerevisiae, which efficiently packages DNA into minichromosomes in a reaction dependent on exogenous core histones and an ATP-regenerating system. Both supercoiled and relaxed plasmid DNA serve as templates for nucleosomal loading i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh107
更新日期：2004-07-28 00:00:00
abstract：:Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh736
更新日期：2004-08-03 00:00:00
abstract：:Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleoso...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1240
更新日期：2017-04-07 00:00:00
abstract：:We describe the further development of a widely used package of DNA/protein sequence analysis programs (1). Important revisions have been made based on user experience, and new features, multi-user capability, and a set of large scale homology programs have been added. The programs are very user friendly, economical o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.1part2.643
更新日期：1984-01-11 00:00:00
abstract：:We have developed a new method for analysis of complex genetic systems by a combination of the Amplification Refractory Mutation System (ARMS) and Single-Strand Conformation Polymorphism (SSCP) analysis: ARMS-SSCP. Thus, a complex allelic series is subdivided into a number of groups by ARMS followed by the identificat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.5.1005
更新日期：1992-03-11 00:00:00
abstract：:To synchronize SV40 replicons, simian cells infected with a tsA mutant were restricted at 40 degrees, to complete ongoing replication and returned to 32 degrees, to activate new replicons in the presence of the DNA chain elongation inhibitor aphidicolin. Upon further incubation at 40 degrees without the drug, 3H-dT wa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.2.763
更新日期：1982-01-22 00:00:00
abstract：:Myotonic dystrophy type 1 (DM1) is an autosomal dominant multisystemic disorder caused by expansion of CTG triplet repeats in 3'-untranslated region of DMPK gene. The pathomechanism of DM1 is driven by accumulation of toxic transcripts containing expanded CUG repeats (CUG(exp)) in nuclear foci which sequester several ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv163
更新日期：2015-03-31 00:00:00
abstract：:Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz860
更新日期：2020-01-08 00:00:00
abstract：:OGG1 initiated base excision repair (BER) is the major pathway for repair of oxidative DNA base damage 8-oxoguanine (8-oxoG). Here, we report that RECQL4 DNA helicase, deficient in the cancer-prone and premature aging Rothmund-Thomson syndrome, physically and functionally interacts with OGG1. RECQL4 promotes catalytic...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa392
更新日期：2020-07-09 00:00:00
abstract：:The functionalisation of oligodeoxynucleotides and oligoribonucleotides by incorporation of 2'-amido-2'-deoxyribonucleosides, possibly containing a reporter group via the 2'-amido bond, was examined. Therefore 2'-acetamido-ribonucleosides containing a small methyl group at the 2'-amido bond were synthesized as model c...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.1.51
更新日期：1995-01-11 00:00:00
abstract：:Competition binding and UV melting studies of a DNA model system consisting of three, four or five mutually complementary oligonucleotides demonstrate that unpaired bases at the branch point stabilize three- and five-way junction loops but destabilize four-way junctions. The inclusion of unpaired nucleotides permits t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.12.2212
更新日期：1995-06-25 00:00:00
abstract：:Using a partially purified replication complex from tobacco chloroplasts, replication origins have been localized to minimal sequences of 82 (pKN8, positions 137 683-137 764) and 243 bp (pKN3, positions 130 513-130 755) for ori A and ori B respectively. Analysis of in vitro replication products by two-dimensional agar...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.18.3681
更新日期：1997-09-15 00:00:00
abstract：:Sets of genes expressed in the same tissue are believed to be under the regulation of a similar set of transcription factors, and can thus be assumed to contain similar structural patterns in their regulatory regions. Here we present a study of the structural patterns in promoters of genes expressed specifically in 26...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp866
更新日期：2010-01-01 00:00:00
abstract：:The archaeal and eukaryotic evolutionary domains diverged from each other approximately 2 billion years ago, but many of the core components of their transcriptional and translational machineries still display a readily recognizable degree of similarity in their primary structures. The F and P subunits present in arch...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.21.4299
更新日期：2000-11-01 00:00:00
abstract：:The current methods for quantifying genome-wide 5-methylcytosine (5mC) oxides are still scarce, mostly restricted with two limitations: assay sensitivity is seriously compromised with cost, assay time and sample input; epigenetic information is irreproducible during polymerase chain reaction (PCR) amplification withou...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz704
更新日期：2019-11-04 00:00:00
abstract：:Proper expression of the genes of the human beta-globin gene locus requires the associated locus control region (LCR). Structurally, the LCR is defined by the presence of four domains of erythroid-specific chromatin structure. These domains, which have been characterized as DNase I hypersensitive sites (HSs), comprise...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.24.5684
更新日期：1998-12-15 00:00:00
abstract：:A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks451
更新日期：2012-08-01 00:00:00
abstract：:The photostability and narrow emission spectra of non-organic quantum dot fluorophores (QDs) make them desirable candidates for fluorescent in situ hybridization (FISH) to study the expression of specific mRNA transcripts. We developed a novel method for direct QD labeling of modified oligonucleotide probes through st...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni162
更新日期：2005-10-13 00:00:00
abstract：:MAGIA(2) (http://gencomp.bio.unipd.it/magia2) is an update, extension and evolution of the MAGIA web tool. It is dedicated to the integrated analysis of in silico target prediction, microRNA (miRNA) and gene expression data for the reconstruction of post-transcriptional regulatory networks. miRNAs are fundamental post...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks460
更新日期：2012-07-01 00:00:00
abstract：:Nuclear extracts prepared from growth hormone-secreting (GC) and prolactin-secreting (235-1) rat anterior pituitary cell lines were compared for their ability to bind to the DNA sequences conferring tissue-specificity to the expression of the rat growth hormone (rGH) gene promoter. Cell-specific differences in the int...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.17.5235
更新日期：1990-09-11 00:00:00
abstract：:Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference....
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm195
更新日期：2007-01-01 00:00:00
abstract：:Stacking self-association equilibria in aqueous solutions of m3uridine, m34,2',3',5'uridine, 2'-deoxyuridine, m13,4,4cytosine, m14,4,4,5cytosine, s2cytidine and s4thymidine were studied at various temperatures by vapour-pressure osmometry. Equilibrium constants Kst's were computed on the assumption of the isodesmic mo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.5.1295
更新日期：1976-05-01 00:00:00
abstract：:Synthetic DNA fragments were constructed to determine the effect of G tracts, in conjunction with periodically spaced A tracts, on DNA bends. Relative length measurements showed that the G tracts spaced at the half helical turn enhanced the DNA bend. When the G tract was interrupted with a thymine or shortened to one ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.4.817
更新日期：1990-02-25 00:00:00
abstract：:PubChem (http://pubchem.ncbi.nlm.nih.gov) is a public repository for biological activity data of small molecules and RNAi reagents. The mission of PubChem is to deliver free and easy access to all deposited data, and to provide intuitive data analysis tools. The PubChem BioAssay database currently contains 500,000 des...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1132
更新日期：2012-01-01 00:00:00
abstract：:Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg910
更新日期：2003-12-01 00:00:00
