Abstract:
:Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's about 40 degrees lower than the unmodified parent compound, d(CG)3. Moreover, the presence of the (O6Me)G appears to inhibit the B leads to Z transition, as determined by CD spectroscopy.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kuzmich S,Marky LA,Jones RAdoi
10.1093/nar/11.10.3393subject
Has Abstractpub_date
1983-05-25 00:00:00pages
3393-403issue
10eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::Muscle creatine kinase (MCK) is a major enzyme of cellular energy metabolism that is expressed upon differentiation of myoblasts into myotubes. Previously we cloned and sequenced the entire rabbit enzyme cDNA which was used as a probe in these studies to obtain a genomic clone from a rabbit library. The transcription ...
journal_title:Nucleic acids research
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abstract::Histone proteins are synthesized in large amounts during S-phase to package the newly replicated DNA, and are among the most stable proteins in the cell. The replication-dependent (RD)-histone mRNAs expressed during S-phase end in a conserved stem-loop rather than a polyA tail. In addition, there are replication-indep...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw620
更新日期:2016-11-02 00:00:00
abstract::Motif3D is a web-based protein structure viewer designed to allow sequence motifs, and in particular those contained in the fingerprints of the PRINTS database, to be visualised on three-dimensional (3D) structures. Additional functionality is provided for the rhodopsin-like G protein-coupled receptors, enabling finge...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.21.8815
更新日期:1987-11-11 00:00:00
abstract::Cells of the central nervous system (CNS) are prone to the devastating consequences of trinucleotide repeat (TNR) expansion. Some CNS cells, including astrocytes, show substantial TNR instability in affected individuals. Since astrocyte enrichment occurs in brain regions sensitive to neurodegeneration and somatic TNR ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl614
更新日期:2006-01-01 00:00:00
abstract::E. coli genes for tRNAasp and tRNAtrp are transcribed by eukaryotic RNA polymerase III upon injection into nuclei of Xenopus oocytes. This indicates that some bacterial tRNA genes contain RNA polymerase III promoter sequences. We discuss evolutionary aspects of this finding. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.22.7153
更新日期:1982-11-25 00:00:00
abstract::Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its processivity during plasmid unwinding. Here we show that RepD forms a co...
journal_title:Nucleic acids research
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doi:10.1093/nar/gkp1153
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki168
更新日期:2005-01-12 00:00:00
abstract::MicroRNAs (miRNAs) are small RNAs ∼22 nt in length that are involved in the regulation of a variety of physiological and pathological processes. Advances in high-throughput small RNA sequencing (smRNA-seq), one of the next-generation sequencing applications, have reshaped the miRNA research landscape. In this study, w...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
abstract::Flap endonuclease-1 (FEN1) is a member of the Rad2 structure-specific nuclease family. FEN1 possesses FEN, 5'-exonuclease and gap-endonuclease activities. The multiple nuclease activities of FEN1 allow it to participate in numerous DNA metabolic pathways, including Okazaki fragment maturation, stalled replication fork...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkq884
更新日期:2011-02-01 00:00:00
abstract::Protein-protein interactions regulate many essential enzymatic processes in the cell. Somatic mutations outside of an enzyme active site can therefore impact cellular function by disruption of critical protein-protein interactions. In our investigation of the cellular impact of the T304I cancer mutation of DNA Polymer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz293
更新日期:2019-07-09 00:00:00
abstract::The signal recognition particle database (SRPDB) is located at the University of Texas Health Science Center at Tyler and includes tabulations of SRP RNA, SRP protein and SRP receptor sequences. The sequences are annotated with links to the primary databases. They are ordered alphabetically or phylogenetically and are...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.169
更新日期:1999-01-01 00:00:00
abstract::Understanding networks of protein-protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking for the subsequent ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm563
更新日期:2007-01-01 00:00:00
abstract::The Yeast Protein Localization database (YPL.db) contains information about the localization patterns of yeast proteins resulting from microscopic analyses. The data and parameters of the experiments to obtain the localization information, together with images from confocal or video microscopy, are stored in a relatio...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.1.80
更新日期:2002-01-01 00:00:00
abstract::Recent studies have employed cross-species comparisons of transcription factor binding, reporting significant regulatory network 'rewiring' between species. Here, we address how a transcriptional repressor targets and regulates neural genes differentially between human and mouse embryonic stem cells (ESCs). We find th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv514
更新日期:2015-07-13 00:00:00
abstract::Telomeric DNAs consist of tandem repeats of G-clusters such as TTAGGG and TG1-3, which are the human and yeast repeat sequences, respectively. In the yeast Saccharomyces cerevisiae, the telomeric repeats are non-nucleosomal, whereas in humans, they are organized in tightly packaged nucleosomes. However, previous in vi...
journal_title:Nucleic acids research
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更新日期:2014-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.3.713
更新日期:1993-02-11 00:00:00
abstract::Synonymous codon usage significantly impacts translational and transcriptional efficiency, gene expression, the secondary structure of both mRNA and proteins, and has been implicated in various diseases. However, population-specific differences in codon usage biases remain largely unexplored. Here, we present a web se...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa863
更新日期:2020-11-04 00:00:00
abstract::Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (AAF-dG) [F. Boudsocq, S. Iwai, F. Hanaoka and R. Woodgate (2001) Nucleic Acids Res., 29, 4607-4616]. Our goal is to elucidate...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-08-22 00:00:00
abstract::The IMGT/HLA database (http://www.ebi.ac.uk/imgt/hla) has provided a centralized repository for the sequences of the alleles named by the WHO Nomenclature Committee for Factors of the HLA System for the past four years. Since its initial release the database has grown and is the primary source of information for the s...
journal_title:Nucleic acids research
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更新日期:2003-01-01 00:00:00
abstract::Revealing the clonal composition of a single tumor is essential for identifying cell subpopulations with metastatic potential in primary tumors or with resistance to therapies in metastatic tumors. Sequencing technologies provide only an overview of the aggregate of numerous cells. Computational approaches to de-mix a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt641
更新日期:2013-09-01 00:00:00
abstract::Emerging evidence suggests that recombinant adeno-associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an rAAV vector construction procedure employing fusion PCR and a single cloning step that considerably simplifies the knockout process. We demonstrate its ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh009
更新日期:2004-01-02 00:00:00
abstract::The three CD3 genes on human chromosome 11q23 encode proteins (gamma, delta and epsilon) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.3.459
更新日期:1990-02-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-08-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1148
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2017-12-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.18.7175
更新日期:1986-09-25 00:00:00