The DEAD box RNA helicase family in Arabidopsis thaliana.

abstract：:The effect of mutations in the highly conserved Y-GG/A motif of B-type DNA polymerases was studied in the DNA polymerase from the hyperthermophilic euryarchaeon Thermococcus aggregans. This motif plays a critical role in the balance between the synthesis and degradation of the DNA chain. Five different mutations of th...

journal_title：Nucleic acids research

authors： Böhlke K,Pisani FM,Vorgias CE,Frey B,Sobek H,Rossi M,Antranikian G

更新日期：2000-10-15 00:00:00

abstract：:Apolipoprotein B (apoB) mRNA editing is a nuclear event that minimally requires the RNA substrate, APOBEC-1 and APOBEC-1 Complementation Factor (ACF). The co-localization of these macro-molecules within the nucleus and the modulation of hepatic apoB mRNA editing activity have been described following a variety of meta...

journal_title：Nucleic acids research

authors： Lehmann DM,Galloway CA,Sowden MP,Smith HC

更新日期：2006-07-04 00:00:00

abstract：:Alternative splicing is widespread in mammalian gene expression, and variant splice patterns are often specific to different stages of development, particular tissues or a disease state. There is a need to systematically collect data on alternatively spliced exons, introns and splice isoforms, and to annotate this dat...

journal_title：Nucleic acids research

authors： Thanaraj TA,Stamm S,Clark F,Riethoven JJ,Le Texier V,Muilu J

更新日期：2004-01-01 00:00:00

abstract：:mRNA deadenylation is under the control of cis-acting regulatory elements, which include AU-rich elements (AREs) and microRNA (miRNA) targeting sites, within the 3' untranslated region (3' UTRs) of eukaryotic mRNAs. Deadenylases promote miRNA-induced mRNA decay through their interaction with miRNA-induced silencing co...

journal_title：Nucleic acids research

authors： Zhang X,Devany E,Murphy MR,Glazman G,Persaud M,Kleiman FE

更新日期：2015-12-15 00:00:00

abstract：:A human telomere ends in a single-stranded 3' tail, composed of repeats of T2AG3. G-quadruplexes (GQs) formed from four consecutive repeats have been shown to possess high-structural and mechanical diversity. In principle, a GQ can form from any four repeats that are not necessarily consecutive. To understand the dyna...

journal_title：Nucleic acids research

authors： Mitra J,Ha T

更新日期：2019-12-02 00:00:00

abstract：:Competition binding and UV melting studies of a DNA model system consisting of three, four or five mutually complementary oligonucleotides demonstrate that unpaired bases at the branch point stabilize three- and five-way junction loops but destabilize four-way junctions. The inclusion of unpaired nucleotides permits t...

journal_title：Nucleic acids research

authors： Kadrmas JL,Ravin AJ,Leontis NB

更新日期：1995-06-25 00:00:00

abstract：:In mammalian cells, DNA double-strand breaks (DSBs) are repaired by three pathways, nonhomologous end-joining (NHEJ), gene conversion (GC) and single-strand annealing (SSA). These pathways are distinct with regard to repair efficiency and mutagenic potential and must be tightly controlled to preserve viability and gen...

journal_title：Nucleic acids research

authors： Mansour WY,Schumacher S,Rosskopf R,Rhein T,Schmidt-Petersen F,Gatzemeier F,Haag F,Borgmann K,Willers H,Dahm-Daphi J

更新日期：2008-07-01 00:00:00

abstract：:Mitochondrial transcription factor A (mtTFA/TFAM) is a nucleus-encoded, high-mobility-group-box (HMG-box) protein that regulates transcription of the mitochondrial genome by specifically recognizing light-strand and heavy-strand promoters (LSP, HSP1). TFAM also binds mitochondrial DNA in a non-sequence specific (NSS) ...

journal_title：Nucleic acids research

authors： Malarkey CS,Bestwick M,Kuhlwilm JE,Shadel GS,Churchill ME

更新日期：2012-01-01 00:00:00

abstract：:The alcohol dehydrogenase (Adh) gene of D. melanogaster is transcribed from two different promoters during fly development: the distal (adult) and the proximal (embryonic-larval). Certain aspects of Adh gene regulation are represented in Drosophila continuous cell lines. We have used Drosophila tissue culture cells in...

journal_title：Nucleic acids research

authors： Benyajati C,Ayer S,McKeon J,Ewel A,Huang J

更新日期：1987-10-12 00:00:00

abstract：:DNA damage-induced cell cycle checkpoints serve as surveillance mechanisms to maintain genomic stability, and are regulated by ATM/ATR-mediated signaling pathways that are conserved from yeast to humans. Trypanosoma brucei, an early divergent microbial eukaryote, lacks key components of the conventional DNA damage-ind...

journal_title：Nucleic acids research

authors： Zhou Q,Pham KTM,Hu H,Kurasawa Y,Li Z

更新日期：2019-09-05 00:00:00

abstract：:Wybutine (Ywye), situated next to the 3'-side of the anticodon of tRNAPhe from Saccharomyces cerevisiae, can be photo-crosslinked to mRNA when bound to Escherichia coli ribosomes. Crosslinking can be obtained with poly(U) as well as with oligonucleotides such as pAUGUUU or p(U)6. In order to identify the site of react...

journal_title：Nucleic acids research

authors： Steiner G,Lührmann R,Kuechler E

更新日期：1984-11-12 00:00:00

abstract：:Cpf1 nucleases were recently reported to be highly specific and programmable nucleases with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA and recognize a 5'-TTTN-3' protospacer adjacent motif (PAM) at the 5' end of the protospacer for genome editing. For widespread application in...

journal_title：Nucleic acids research

authors： Tu M,Lin L,Cheng Y,He X,Sun H,Xie H,Fu J,Liu C,Li J,Chen D,Xi H,Xue D,Liu Q,Zhao J,Gao C,Song Z,Qu J,Gu F

更新日期：2017-11-02 00:00:00

abstract：:Metagenomics has been employed to systematically sequence, classify, analyze and manipulate the entire genetic material isolated from environmental samples. Finding genes within metagenomic sequences remains a formidable challenge, and noncoding RNA genes other than those encoding rRNA and tRNA are not well annotated ...

journal_title：Nucleic acids research

authors： Zhu Y,Pulukkunat DK,Li Y

更新日期：2007-01-01 00:00:00

abstract：:The Diels-Alder reaction between diene-modified oligonucleotides and maleimide-derivatized peptides afforded peptide-oligonucleotide conjugates with high purity and yield. Synthesis of the reagents was easily accomplished by on-column derivatization of the corresponding peptides and oligonucleotides. The cycloaddition...

journal_title：Nucleic acids research

authors： Marchán V,Ortega S,Pulido D,Pedroso E,Grandas A

更新日期：2006-02-14 00:00:00

abstract：:Histone post-translational modifications regulate chromatin structure and function largely through interactions with effector proteins that often contain multiple histone-binding domains. PHF1 [plant homeodomain (PHD) finger protein 1], which contains two kinds of histone reader modules, a Tudor domain and two PHD fin...

journal_title：Nucleic acids research

authors： Liu R,Gao J,Yang Y,Qiu R,Zheng Y,Huang W,Zeng Y,Hou Y,Wang S,Leng S,Feng D,Yu W,Sun G,Shi H,Teng X,Wang Y

更新日期：2018-07-27 00:00:00

abstract：:DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable 'scar' sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinato...

journal_title：Nucleic acids research

authors： Taylor GM,Mordaka PM,Heap JT

更新日期：2019-02-20 00:00:00

abstract：:Rad54 protein is a key member of the RAD52 epistasis group required for homologous recombination in eukaryotes. Rad54 is a duplex DNA translocase that remodels both DNA and protein-DNA complexes, and functions at multiple steps in the recombination process. Here we use biochemical criteria to demonstrate the existence...

journal_title：Nucleic acids research

authors： Haseltine CA,Kowalczykowski SC

更新日期：2009-05-01 00:00:00

abstract：:DNA primase has been partially purified from wheat germ. This enzyme, like DNA primases characterized from many procaryotic and eucaryotic sources, catalyses the synthesis of primers involved in DNA replication. However, the wheat enzyme differs from animal DNA primase in that it is found partially associated with a D...

journal_title：Nucleic acids research

authors： Laquel P,Castroviejo M,Litvak S

更新日期：1990-08-25 00:00:00

abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...

journal_title：Nucleic acids research

authors： Faust M,Hastings KE,Millward S

更新日期：1975-08-01 00:00:00

abstract：:Effective transcript profiling in animal systems requires isolation of homogenous tissue or cells followed by faithful mRNA amplification. Linear amplification based on cDNA synthesis and in vitro transcription is reported to maintain representation of mRNA levels, however, quantitative data demonstrating this as well...

journal_title：Nucleic acids research

authors： Baugh LR,Hill AA,Brown EL,Hunter CP

更新日期：2001-03-01 00:00:00

abstract：:Many proteins of the SNF2 family, which share a similar DNA-dependent ATPase/putative helicase domain, are involved in global transcriptional control and processing of DNA damage. We report here the partial cloning and characterization of 89B helicase, a gene encoding a new Drosophila melanogaster member of the SNF2 f...

journal_title：Nucleic acids research

authors： Goldman-Levi R,Miller C,Bogoch J,Zak NB

更新日期：1996-08-15 00:00:00

abstract：:The locus DXS269 (P20) defines a deletion hotspot in the distal part of the Duchenne Muscular Dystrophy gene. We have cloned over 90 kilobase-pairs of genomic DNA from this region in overlapping cosmids. The use of whole cosmids as probes in a competitive DNA hybridization analysis proves a fast and convenient method ...

journal_title：Nucleic acids research

authors： Blonden LA,den Dunnen JT,van Paassen HM,Wapenaar MC,Grootscholten PM,Ginjaar HB,Bakker E,Pearson PL,van Ommen GJ

更新日期：1989-07-25 00:00:00

abstract：:In this article, we characterize histone demethylase activity of the entire family of JmjC+N proteins of Drosophila melanogaster. Our results show that Lid (little imaginal discs), which is structurally homologous to JARID1, demethylates H3K4me3. However, contrary to what would be inferred from its demethylase activit...

journal_title：Nucleic acids research

authors： Lloret-Llinares M,Carré C,Vaquero A,de Olano N,Azorín F

更新日期：2008-05-01 00:00:00

abstract：:Embryonic stem cells (ESCs) and meiosis are featured by relatively higher frequent homologous recombination associated with DNA double strand breaks (DSB) repair. Here, we show that Pold3 plays important roles in DSB repair, telomere maintenance and genomic stability of both ESCs and spermatocytes in mice. By attempti...

journal_title：Nucleic acids research

authors： Zhou Z,Wang L,Ge F,Gong P,Wang H,Wang F,Chen L,Liu L

更新日期：2018-04-20 00:00:00

abstract：:A fundamental problem with applying Consensus, Weight-Matrix or hidden Markov models as search tools for biosequences is that there is no way to know, from the model, if the modeled sequences display any dependencies between positional identities. In some instances, these dependencies are crucial in correctly acceptin...

journal_title：Nucleic acids research

authors： Ray WC

更新日期：2005-07-01 00:00:00

abstract：:The polymerase encoded by human hepatitis B virus, which has reverse transcriptase and RNase H activity, binds to its pregenomic RNA template in a two-step process involving a terminal redundancy. Both first strand and second strand DNA synthesis involve primer translocation and second strand synthesis involves a temp...

journal_title：Nucleic acids research

authors： Kidd AH,Kidd-Ljunggren K

更新日期：1996-09-01 00:00:00

abstract：:Bacterial small RNAs (sRNAs) are a heterogeneous group of post-transcriptional regulators that often act at the heart of large networks. Hundreds of sRNAs have been discovered by genome-wide screens and most of these sRNAs exert their functions by base-pairing with target mRNAs. However, studies addressing the molecul...

journal_title：Nucleic acids research

authors： Fröhlich KS,Förstner KU,Gitai Z

更新日期：2018-11-16 00:00:00

abstract：:Giant chromatin-modifying complexes regulate gene transcription in eukaryotes by acting on chromatin substrates and 'setting' the histone code. The histone deacetylase (HDAC)-associated mammalian Sin3 corepressor complex regulates a wide variety of genes involved in all aspects of cellular physiology. The recruitment ...

journal_title：Nucleic acids research

authors： He Y,Imhoff R,Sahu A,Radhakrishnan I

更新日期：2009-04-01 00:00:00

abstract：:The interaction of antibodies directed toward N6-(delta2-isopentenyl)adenosine, i6Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoassay to quantitate th...

journal_title：Nucleic acids research

authors： Milstone DS,Vold BS,Glitz DG,Shutt N

更新日期：1978-09-01 00:00:00

abstract：:Overexpressed in colon carcinoma-1 (OCC-1) is one of the earliest annotated long noncoding RNAs (lncRNAs) in colorectal cancer (CRC); however, its function remains largely unknown. Here, we revealed that OCC-1 plays a tumor suppressive role in CRC. OCC-1 knockdown by RNA interference promotes cell growth both in vitro...

journal_title：Nucleic acids research

authors： Lan Y,Xiao X,He Z,Luo Y,Wu C,Li L,Song X

更新日期：2018-06-20 00:00:00