Abstract：
:Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The restriction cleavage map and the DNA sequence of a selected part of the inserted DNA are in complete accordance with the amino-acid sequence of soybean leghemoglobin.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Truelsen E,Gausing K,Jochimsen B,Jørgensen P,Marcker KAdoi
10.1093/nar/6.9.3061subject
Has Abstractpub_date
1979-07-11 00:00:00pages
3061-72issue
9eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract：:The field bean, Vicia faba L. var. minor, possesses two sub-families of 11 S legumin genes named A and B. We isolated from a genomic library a B-type gene (LeB4) and determined its primary DNA sequence. Gene LeB4 codes for a 484 amino acid residue prepropolypeptide, encompassing a signal peptide of 22 amino acid resid...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.6.2707
更新日期：1986-03-25 00:00:00
abstract：:This is a study of the effect of total and partial deuteration of solvent on critical temperatures and profiles of all four reactions occurring in poly(A) x n poly(U) (n = 1 or 2) aqueous systems. The study was done at observational times not longer than hydrogen exchange times at base pairs in helically ordered struc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.18.4283
更新日期：1980-09-25 00:00:00
abstract：:The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.22.4335
更新日期：1999-11-15 00:00:00
abstract：:Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks927
更新日期：2012-12-01 00:00:00
abstract：:Programmed chromosome breakage occurs at 50-200 specific sites in the genome of Tetrahymena thermo-phila during somatic nuclear (macronuclear) differentiation. Previous studies have identified a 15 bp sequence, the Cbs (for chromosome breakage sequence), that is necessary and sufficient to specify these sites. In this...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.4.895
更新日期：2000-02-15 00:00:00
abstract：:The miRNA Registry provides a service for the assignment of miRNA gene names prior to publication. A comprehensive and searchable database of published miRNA sequences is accessible via a web interface (http://www.sanger.ac.uk/Software/Rfam/mirna/), and all sequence and annotation data are freely available for downloa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh023
更新日期：2004-01-01 00:00:00
abstract：:DNA sequences, that control expression of the spliced leader (SL) RNA gene in the parasitic protozoan Leishmania amazonensis, were mapped by block substitution mutagenesis. In the absence of a functional in vitro system for transcription, no promoter elements have yet been identified in this organism. We therefore dev...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.11.1959
更新日期：1994-06-11 00:00:00
abstract：:The availability of the complete sequence of the Saccharomyces cerevisiae genome has allowed a comprehensive analysis of the genes encoding cytoplasmic ribosomal proteins in this organism. On the basis of this complete inventory a new nomenclature for the yeast ribosomal proteins is presented. ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.24.4872
更新日期：1997-12-15 00:00:00
abstract：:Telomerase activity, not detectable in somatic cells but frequently activated during carcinogenesis, confers immortality to tumors. Mechanisms governing expression of the catalytic subunit hTERT, the limiting factor for telomerase activity, still remain unclear. We previously proposed a model in which the binding of t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq827
更新日期：2011-02-01 00:00:00
abstract：:We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by compa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.20.7705
更新日期：1984-10-25 00:00:00
abstract：:Underlying higher order chromatin organization are Structural Maintenance of Chromosomes (SMC) complexes, large protein rings that entrap DNA. The molecular mechanism by which SMC complexes organize chromatin is as yet incompletely understood. Two prominent models posit that SMC complexes actively extrude DNA loops (l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1270
更新日期：2021-01-12 00:00:00
abstract：:We investigate the role of water molecules in 89 protein-RNA complexes taken from the Protein Data Bank. Those with tRNA and single-stranded RNA are less hydrated than with duplex or ribosomal proteins. Protein-RNA interfaces are hydrated less than protein-DNA interfaces, but more than protein-protein interfaces. Majo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku679
更新日期：2014-09-01 00:00:00
abstract：:Transposable elements (TEs) comprise a large proportion of long non-coding RNAs (lncRNAs). Here, we employed CRISPR to delete a short interspersed nuclear element (SINE) in Malat1, a cancer-associated lncRNA, to investigate its significance in cellular physiology. We show that Malat1 with a SINE deletion forms diffuse...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1176
更新日期：2020-03-18 00:00:00
abstract：:Genome length complementary DNA (cDNA) transcripts were synthesized in vitro by using purified virions of a cloned isolate of mouse sarcoma virus (MSV Clone 124). The cDNA transcripts were converted to double-stranded form by utilizing DNase-digested calf thymus DNA primers and E. coli DNA polymerase I. Restriction en...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.5.1863
更新日期：1979-01-01 00:00:00
abstract：:Endonucleases that generate double-strand breaks in DNA often possess two identical subunits related by rotational symmetry, arranged so that the active sites from each subunit act on opposite DNA strands. In contrast to many endonucleases, Type IIP restriction enzyme BcnI, which recognizes the pseudopalindromic seque...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr588
更新日期：2011-11-01 00:00:00
abstract：:The channel proteins belonging to the major intrinsic proteins (MIP) superfamily are diverse and are found in all forms of life. Water-transporting aquaporin and glycerol-specific aquaglyceroporin are the prototype members of the MIP superfamily. MIPs have also been shown to transport other neutral molecules and gases...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr914
更新日期：2012-01-01 00:00:00
abstract：:Investigation of biological processes using selective chemical interventions is generally applied in biomedical research and drug discovery. Many studies of this kind make use of gene expression experiments to explore cellular responses to chemical interventions. Recently, some research groups constructed libraries of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn610
更新日期：2008-11-01 00:00:00
abstract：:An essential coordinator of all DNA metabolic processes is Replication Protein A (RPA). RPA orchestrates these processes by binding to single-stranded DNA (ssDNA) and interacting with several other DNA binding proteins. Determining the real-time kinetics of single players such as RPA in the presence of multiple DNA pr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx598
更新日期：2017-09-19 00:00:00
abstract：:Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism with respect to global protein-protein networks. Yet, there has been no global census of RNA/protein complexes in this model species of microbiology. Here,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa676
更新日期：2020-09-18 00:00:00
abstract：:The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm817
更新日期：2007-01-01 00:00:00
abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku130
更新日期：2014-04-01 00:00:00
abstract：:The COXPRESdb (http://coxpresdb.jp) provides gene coexpression relationships for animal species. Here, we report the updates of the database, mainly focusing on the following two points. For the first point, we added RNAseq-based gene coexpression data for three species (human, mouse and fly), and largely increased th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku1163
更新日期：2015-01-01 00:00:00
abstract：:Synthesis and antisense activity of oligonucleotides modified with 2'-O-[2-[(N,N-dimethylamino)oxy] ethyl] (2'-O-DMAOE) are described. The 2'-O-DMAOE-modified oligonucleotides showed superior metabolic stability in mice. The phosphorothioate oligonucleotide 'gapmers', with 2'-O-DMAOE- modified nucleoside residues at t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh220
更新日期：2004-02-03 00:00:00
abstract：:The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.12.2105
更新日期：1995-06-25 00:00:00
abstract：:The genomic distribution of the abundant eukaryotic d(GA x TC)(n) DNA microsatellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombination in SV40 minichromosomes, the rate of homologous DNA recombination increasing by as much...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.23.4617
更新日期：2000-12-01 00:00:00
abstract：:The transcriptional promoter of satellite 2 from the eastern newt, Notophthalmus viridescens, was analyzed by assaying the activity of deleted or mutated satellite 2 clones in Xenopus laevis oocytes. Two elements in the promoter were found to be important for transcription. These elements have sequences that are simil...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.22.4697
更新日期：1994-11-11 00:00:00
abstract：:A dnaB-like protein from P. aeruginosa was purified to near homogeneity using as an assay the immunoprecipitation by E. coli dnaB antiserum in a solid-phase. In the chromatographic characteristics including the affinity to immobilized ATP the dnaB-like protein of P. aeruginosa is similar to the dnaB protein of E. coli...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.2.385
更新日期：1987-01-26 00:00:00
abstract：:The triphosphate of the nucleoside deoxyribosyl dihydropyrimido[4,5-c][1,2]oxazin-7-one (dP) is known to be incorporated into DNA efficiently by Taq polymerase and is a useful tool for polymerase-mediated in vitro mutagenesis. It is shown here that dP is a potent mutagen in Escherichia coli and Salmonella typhimurium ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.8.1548
更新日期：1997-04-15 00:00:00
abstract：:We present the PATHOgenic YEAst Search for Transcriptional Regulators And Consensus Tracking (PathoYeastract - http://pathoyeastract.org) database, a tool for the analysis and prediction of transcription regulatory associations at the gene and genomic levels in the pathogenic yeasts Candida albicans and C. glabrata Up...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw817
更新日期：2017-01-04 00:00:00
abstract：:Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.6.1317
更新日期：1997-03-15 00:00:00
