Abstract:
:Work from a number of laboratories has indicated that the TATA box sequence can act as a basal promoter element not only for RNA polymerase II (RNAP II) transcription, but also for transcription by RNA polymerase III (RNAP III). We previously reported that, in the absence of other cis-acting elements, the canonical TATA sequence TATAAAAA specifically supported transcription by RNAP II in an unfractionated Drosophila nuclear extract, whereas the sequence TTTTTATA (the same sequence in reverse orientation) directed RNAP III transcription. We have now examined a variety of other TATA box sequences with regard to RNA polymerase selectivity and their ability to support RNAP III transcription. The results have allowed us to rank these TATA box sequences with respect to their relative strengths as RNAP III promoter elements in unfractionated Drosophila extracts. Further, the data indicate that T residues at positions 2 and 4 of the TATA box appear to be important determinants of RNAP III selectivity in this system, whereas A residues at these positions favor RNAP II transcription. Finally, the data suggest that transcription factors TFIID and TFIIIB, although both capable of binding a variety of TATA elements, have distinct sequence preferences for recognizing the TATA box and possibly the surrounding DNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Wang Y,Jensen RC,Stumph WEdoi
10.1093/nar/24.15.3100subject
Has Abstractpub_date
1996-08-01 00:00:00pages
3100-6issue
15eissn
0305-1048issn
1362-4962pii
6g0080journal_volume
24pub_type
杂志文章abstract::The archaeal exosome is a phosphorolytic 3'-5' exoribonuclease complex. In a reverse reaction it synthesizes A-rich RNA tails. Its RNA-binding cap comprises the eukaryotic orthologs Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. In Sulfolobus solfataricus DnaG and Rrp4 but not Csl4 show preference f...
journal_title:Nucleic acids research
pub_type: 杂志文章
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abstract::Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type cl...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki077
更新日期:2005-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2018-04-06 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1989-10-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-06-24 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
abstract::Yeast Dna2 helicase/nuclease is essential for DNA replication and assists FEN1 nuclease in processing a subset of Okazaki fragments that have long single-stranded 5' flaps. It is also involved in the maintenance of telomeres. DNA2 is a gene conserved in eukaryotes, and a putative human ortholog of yeast DNA2 (ScDNA2) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl070
更新日期:2006-04-04 00:00:00
abstract::The European Nucleotide Archive (ENA; http://www.ebi.ac.uk/ena) is a repository for the submission, maintenance and presentation of nucleotide sequence data and related sample and experimental information. In this article we report on ENA in 2015 regarding general activity, notable published data sets and major achiev...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1311
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.7.1113
更新日期:1995-04-11 00:00:00
abstract::Human transcription factor Sp1 contains three contiguous repeats of the C2H2-type zinc finger motif and binds to the decanucleotide sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box). In order to determine whether the three-zinc finger peptide Sp1(530-623) has selectivity for sequence outside the GC box, we used a sel...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.24.4920
更新日期:2001-12-15 00:00:00
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更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.13.3941
更新日期:1990-07-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2013-05-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1989-11-25 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2004-01-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1985-01-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-07-13 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
abstract::In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.17.4695
更新日期:1991-09-11 00:00:00
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更新日期:1999-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2017-11-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1264
更新日期:2011-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.24.7259
更新日期:1990-12-25 00:00:00