Abstract:
:The cause of 50S ribosomal subunit collapse reportedly triggered by hybridization of a 14-base cDNA probe to the alpha-sarcin region of 23S rRNA was investigated by physical measurement of probe-subunit complexes in varying buffer conditions. The results reported here show that this probe was unable to hybridize to its target site in the intact 50S subunit and the physical characteristics of 50S subunits remained unchanged in its presence. Subunit collapse was induced in buffer containing 20mM Tris-HCl (pH 7.5), 600 mM NH4Cl, 1 mM MgCl2, 1 mM DTT, and 0.1 mM EDTA in the absence of probe. The probe bound specifically to its target site in the collapsed particle, but did not promote further unfolding. The results demonstrate that a DNA probe bound to the alpha-sarcin region cannot cause the 50S subunit to unfold or cause 23S rRNA to degrade. We suggest that the previously reported collapse was most probably the result of the ionic conditions used.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
White GA,Wood T,Hill WEdoi
10.1093/nar/16.22.10817subject
Has Abstractpub_date
1988-11-25 00:00:00pages
10817-31issue
22eissn
0305-1048issn
1362-4962journal_volume
16pub_type
杂志文章abstract::We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in the fission yeast. An eff...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.17.8603
更新日期:1988-09-12 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz572
更新日期:2019-09-19 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.19.3825
更新日期:1994-09-25 00:00:00
abstract::We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutationa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.12.2318
更新日期:1994-06-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-07-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.9.1906
更新日期:2000-05-01 00:00:00
abstract::FANTOM DB, the database of Functional Annotation of RIKEN Mouse cDNA Clones, is designed to store sequence information of RIKEN full-length enriched mouse cDNA clones, graphical views of sequence analysis results, curated functional annotation information and additional descriptions, including Gene Ontology terms. RIK...
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更新日期:2002-01-01 00:00:00
abstract::E. coli C6 rel- met- cys- was cultured in a fully supplemented medium and in media lacking cysteine or methionine. tRNA isolated from the three cultures containted, respectively, a normal complement of modified nucleosides; a deficiency in thiolated nucleosides and a deficiency in methylated nucleosides. Both sulfur-d...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.5.691
更新日期:1975-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.5.1609
更新日期:1977-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.6.2307
更新日期:1979-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn443
更新日期:2008-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1980-09-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,meta分析
doi:10.1093/nar/gkp093
更新日期:2009-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1117
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-01-29 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky519
更新日期:2019-02-20 00:00:00
abstract::An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with activities toward adenine paired with guanine and 8-oxo-7,8-dihydroguanine (8-oxoG) as a single peak corresponding to a 55...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.6.1355
更新日期:2000-03-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-01-08 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-09-28 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
abstract::The principles of mRNA decoding are conserved among all extant life forms. We present an integrative view of all the interaction networks between mRNA, tRNA and rRNA: the intrinsic stability of codon-anticodon duplex, the conformation of the anticodon hairpin, the presence of modified nucleotides, the occurrence of no...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2016-09-30 00:00:00
abstract::COMPASS is a method for homology detection and local alignment construction based on the comparison of multiple sequence alignments (MSAs). The method derives numerical profiles from given MSAs, constructs local profile-profile alignments and analytically estimates E-values for the detected similarities. Until now, CO...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm293
更新日期:2007-07-01 00:00:00
abstract::DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restric...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.16.6711
更新日期:1986-08-26 00:00:00
abstract::A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.3.599
更新日期:1990-02-11 00:00:00
abstract::Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...
journal_title:Nucleic acids research
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更新日期:2013-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.1.221
更新日期:1978-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.95
更新日期:1999-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00