Abstract：
:Pre-mRNA transcripts in a variety of organisms, including plants, Drosophila and Caenorhabditis elegans, contain introns which are significantly richer in adenosine and uridine residues than their flanking exons. Previous analyses using exonic and intronic replacements between two nonequivalent 5'splice sites in the 469 nt long rbcS3A intron 1 provided the first evidence indicating that, in both tobacco and Drosophila nuclei, 5'splice site selection is strongly influenced by the position of that site relative to the AU transition point between exon and intron. To differentiate between two potential models for 5'splice site recognition, we have expressed a completely different set of intronic and exonic replacement constructs containing identical 5'splice sites upstream of beta-conglycinin intron 4 (115 nt). Mutagenesis and deletion of the upstream 5'splice site demonstrate that intronic AU-rich sequences function by promoting recognition of the most upstream 5'splice site rather than by masking the downstream 5'splice site. Sequence insertions define a role for AG-rich exonic sequences in plant pre-mRNA splicing by demonstrating that an AG-rich element is capable of promoting downstream 5'splice site recognition. We conclude that AU-rich intronic sequences, AG-rich exonic sequences and the 5'splice site itself collectively define 5'intron boundaries in dicot nuclei.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
McCullough AJ,Schuler MAdoi
10.1093/nar/25.5.1071subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1071-7issue
5eissn
0305-1048issn
1362-4962pii
gka185journal_volume
25pub_type
杂志文章abstract：:A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the fo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.11.2413
更新日期：1980-06-11 00:00:00
abstract：:The potent anticancer drug actinomycin D (ActD) functions by intercalating into DNA at GpC sites, thereby interrupting essential biological processes including replication and transcription. Certain neurological diseases are correlated with the expansion of (CGG)n trinucleotide sequences, which contain many contiguous...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt084
更新日期：2013-04-01 00:00:00
abstract：:The polyadenylate binding protein 1 (PABPN1) is a ubiquitously expressed RNA binding protein vital for multiple steps in RNA metabolism. Although PABPN1 plays a critical role in the regulation of RNA processing, mutation of the gene encoding this ubiquitously expressed RNA binding protein causes a specific form of mus...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx786
更新日期：2017-10-13 00:00:00
abstract：:eggNOG is a public database of orthology relationships, gene evolutionary histories and functional annotations. Here, we present version 5.0, featuring a major update of the underlying genome sets, which have been expanded to 4445 representative bacteria and 168 archaea derived from 25 038 genomes, as well as 477 euka...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1085
更新日期：2019-01-08 00:00:00
abstract：:Atomistic molecular dynamics (MD) simulations generate a wealth of information related to the dynamics of proteins. If properly analyzed, this information can lead to new insights regarding protein function and assist wet-lab experiments. Aiming to identify interactions between individual amino acid residues and the r...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky381
更新日期：2018-07-02 00:00:00
abstract：:The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4453
更新日期：1983-07-11 00:00:00
abstract：:The circular dichroism spectra of Hg(II) complexes with native calf thymus DNA, chemically methylated Streptomyces chrysomallus DNA and with Ag(I)-DNA complexes were measured in the region of 220 - 340 nm. As a main result a conversion of the conservative CD spectrum of DNA to a distinct nonconservative type of CD spe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.3.539
更新日期：1977-03-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt165
更新日期：2013-05-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.11.1549
更新日期：1974-11-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1012
更新日期：2009-02-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.4.1767
更新日期：1984-02-24 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni057
更新日期：2005-04-01 00:00:00
abstract：:The catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs) is a member of a sub-family of phosphatidylinositol (PI) 3-kinases termed PIK-related kinases. A distinguishing feature of this sub-family is the presence of a conserved C-terminal region downstream of a PI 3-kinase domain. Mutants defective in DNA-P...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.8.1965
更新日期：1998-04-15 00:00:00
abstract：:mRNA expression involves transcription initiation, elongation and degradation. In cells, these dynamic processes are highly regulated. However, experimental characterization of the dynamic processes in vivo is difficult due to the paucity of methods capable of direct measurements. We present a highly sensitive and ver...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw596
更新日期：2016-10-14 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.8.3317
更新日期：1987-04-24 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm216
更新日期：2007-07-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.8.1906
更新日期：1999-04-15 00:00:00
abstract：:MicroRNA 26a (miR-26a) reduces cell viability in several cancers, indicating that miR-26a could be used as a therapeutic option in patients. We demonstrate that miR-26a not only inhibits G1-S cell cycle transition and promotes apoptosis, as previously described, but also regulates multiple cell cycle checkpoints. We s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx022
更新日期：2017-05-05 00:00:00
abstract：:Genomic DNA of African trypanosomes contains a hypermodified thymidine residue termed base J (beta-d-glucosyl-HOMedU). This modified base is localized primarily to repetitive DNA, namely the telomeres, and is implicated in the regulation of antigenic variation. The base is synthesized in a two-step pathway. Initially,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1067
更新日期：2009-04-01 00:00:00
abstract：:Adenosine-to-inosine (A-to-I) RNA editing is a common post transcriptional modification. It has a critical role in protecting against false activation of innate immunity by endogenous double stranded RNAs and has been associated with various regulatory processes and diseases such as autoimmune and cardiovascular disea...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa305
更新日期：2020-06-19 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.23.5715
更新日期：1980-12-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.20.4029
更新日期：1995-10-25 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.12.4921
更新日期：1984-06-25 00:00:00
abstract：:The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp988
更新日期：2010-01-01 00:00:00
abstract：:Dictyostelium discoideum is of increasing interest as a model eukaryotic cell because its many attributes have recently been expanded to include improved genetic and biochemical manipulability. The ability to transform Dictyostelium using drug resistance as a selectable marker (1) and to gene target by high frequency ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.14.6617
更新日期：1988-07-25 00:00:00
abstract：:The circadian clock comprises transcriptional feedback loops of clock genes. Cryptochromes are essential components of the negative feedback loop in mammals as they inhibit CLOCK-BMAL1-mediated transcription. We purified mouse CRY1 (mCRY1) protein complexes from Sarcoma 180 cells to determine their roles in circadian ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn1013
更新日期：2009-03-01 00:00:00
abstract：:Studies of bacterial chromosomes and plasmids indicate that their replication initiator proteins bind to origins of replication at many double-stranded sites and also at AT-rich regions where single-stranded DNA is exposed during origin opening. Single-strand binding apparently promotes origin opening by stabilizing a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa826
更新日期：2020-11-04 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw945
更新日期：2017-01-04 00:00:00
abstract：:Modification of cytosine plays an important role in epigenetic regulation of gene expression and genome stability. Cytosine is converted to 5-methylcytosine (5mC) by DNA methyltransferase; in turn, 5mC may be oxidized to 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation enzyme. The structural flexibility of D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1210
更新日期：2021-01-25 00:00:00
abstract：:A cross index is presented for using the improved selectivity offered by the Hung and Wensink (Nucl. Acids Res. 12, 1863-1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be jo...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.8.3199
更新日期：1987-04-24 00:00:00
