The human M creatine kinase gene enhancer contains multiple functional interacting domains.

abstract：UNLABELLED:Partial cleavage of DNA with the restriction endonuclease NotI (5'...GC/GGCCGC...3') is an important technique for genomic mapping. However, partial genomic cleavage with this enzyme is impaired by the agarose matrix in which the DNA must be suspended. To solve this problem we have purified the blocking meth...

journal_title：Nucleic acids research

authors： Hanish J,McClelland M

更新日期：1990-06-11 00:00:00

abstract：:The oligomerization state and mode of binding to DNA of the GIY-YIG endonuclease II (EndoII) from bacteriophage T4 was studied using gel filtration and electrophoretic mobility shift assays with a set of mutants previously found to have altered enzyme activity. At low enzyme/DNA ratios all mutants except one bound to ...

journal_title：Nucleic acids research

authors： Lagerbäck P,Andersson E,Malmberg C,Carlson K

更新日期：2009-10-01 00:00:00

abstract：:We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using 29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat concatemers due to s...

journal_title：Nucleic acids research

authors： Osborne RJ,Thornton CA

更新日期：2008-03-01 00:00:00

abstract：:High mobility group protein B1 (HMGB1) binds to the internucleosomal linker DNA in chromatin and abuts the nucleosome. Bending and untwisting of the linker DNA results in transmission of strain to the nucleosome core, disrupting histone/DNA contacts. An interaction between H3 and HMGB1 has been reported. Here we confi...

journal_title：Nucleic acids research

authors： Watson M,Stott K,Fischl H,Cato L,Thomas JO

更新日期：2014-01-01 00:00:00

abstract：:A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoa...

journal_title：Nucleic acids research

authors： Lahoud G,Timoshchuk V,Lebedev A,Arar K,Hou YM,Gamper H

更新日期：2008-12-01 00:00:00

abstract：:Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...

journal_title：Nucleic acids research

authors： Fort P,Rech J,Vie A,Piechaczyk M,Bonnieu A,Jeanteur P,Blanchard JM

更新日期：1987-07-24 00:00:00

abstract：:COMPASS is a method for homology detection and local alignment construction based on the comparison of multiple sequence alignments (MSAs). The method derives numerical profiles from given MSAs, constructs local profile-profile alignments and analytically estimates E-values for the detected similarities. Until now, CO...

journal_title：Nucleic acids research

authors： Sadreyev RI,Tang M,Kim BH,Grishin NV

更新日期：2007-07-01 00:00:00

abstract：:Alternative splicing and polyadenylation were observed pervasively in eukaryotic messenger RNAs. These alternative isoforms could either be consequences of physiological regulation or stochastic noise of RNA processing. To quantify the extent of stochastic noise in splicing and polyadenylation, we analyzed the alterna...

journal_title：Nucleic acids research

authors： Hon CC,Weber C,Sismeiro O,Proux C,Koutero M,Deloger M,Das S,Agrahari M,Dillies MA,Jagla B,Coppee JY,Bhattacharya A,Guillen N

更新日期：2013-02-01 00:00:00

abstract：:Sox proteins belong to the HMG box superfamily of DNA-binding proteins and are found throughout the animal kingdom. They are involved in the regulation of such diverse developmental processes as germ layer formation, organ development and cell type specifi-cation. Hence, deletion or mutation of Sox proteins often resu...

journal_title：Nucleic acids research

authors： Wegner M

更新日期：1999-03-15 00:00:00

abstract：:A rearrangement of the mitochondrial genome of a long lived mutant of Podospora anserina is presented. It consists in the insertion of 191 bp of the LrDNA gene (coding for the large ribosomal RNA) at the junction between exon1 and intron alpha of gene co1 (coding for subunit 1 of cytochrome oxidase). This insertion is...

journal_title：Nucleic acids research

authors： Sainsard-Chanet A,Begel O

更新日期：1990-02-25 00:00:00

abstract：:Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with ...

journal_title：Nucleic acids research

authors： Gubbins EJ,Maurer RA,Hartley JL,Donelson JE

更新日期：1979-03-01 00:00:00

abstract：:We describe the structure (3840 bp) of a novel Euglena gracilis chloroplast ribosomal protein operon that encodes the five genes rpl16-rpl14-rpl5-rps8-rpl36. The gene organization resembles the spc and the 3'-end of the S10 ribosomal protein operons of E. coli. The rpl5 is a new chloroplast gene not previously reporte...

journal_title：Nucleic acids research

authors： Christopher DA,Hallick RB

更新日期：1989-10-11 00:00:00

abstract：:In this report we have analyzed the role of antisense transcription in the control of LEF1 transcription factor expression. A natural antisense transcript (NAT) is transcribed from a promoter present in the first intron of LEF1 gene and undergoes splicing in mesenchymal cells. Although this locus is silent in epitheli...

journal_title：Nucleic acids research

authors： Beltran M,Aparicio-Prat E,Mazzolini R,Millanes-Romero A,Massó P,Jenner RG,Díaz VM,Peiró S,de Herreros AG

更新日期：2015-07-13 00:00:00

abstract：:Invertebrate RNA viruses are targets of the host RNA interference (RNAi) pathway, which limits virus infection by degrading viral RNA substrates. Several insect RNA viruses encode suppressor proteins to counteract this antiviral response. We recently demonstrated that the dsDNA virus Invertebrate iridescent virus 6 (I...

journal_title：Nucleic acids research

authors： Bronkhorst AW,van Cleef KW,Venselaar H,van Rij RP

更新日期：2014-10-29 00:00:00

abstract：:Naturally occurring RNAs are known to exhibit a high degree of modularity, whereby specific structural modules (or motifs) can be mixed and matched to create new molecular architectures. The modular nature of RNA also affords researchers the ability to characterize individual structural elements in controlled syntheti...

journal_title：Nucleic acids research

authors： Mitchell C,Polanco JA,DeWald L,Kress D,Jaeger L,Grabow WW

更新日期：2019-07-09 00:00:00

abstract：:Chromatin fragments released from intact Friend erythroleukemia cell nuclei during limited incubation with micrococcal nuclease, DNase II or DNase I were analyzed to determine the distribution of DNA methyltransferase in chromatin. The enzyme was released in a free form when internucleosomal DNA was digested with micr...

journal_title：Nucleic acids research

authors： Creusot F,Christman JK

更新日期：1981-10-24 00:00:00

abstract：:RNase P RNA (RPR), the catalytic subunit of the essential RNase P ribonucleoprotein, removes the 5' leader from precursor tRNAs. The ancestral eukaryotic RPR is a Pol III transcript generated with mature termini. In the branch of the arthropod lineage that led to the insects and crustaceans, however, a new allele aros...

journal_title：Nucleic acids research

authors： Palsule G,Gopalan V,Simcox A

更新日期：2019-09-19 00:00:00

abstract：:The hypoxia-inducible factor-1 (HIF-1) was first described as a DNA binding activity that specifically recognizes an 8 bp motif known to be essential for hypoxia-inducible erythropoietin gene transcription. Subsequently HIF-1 activity has also been found in cell lines which do not express erythropoietin, suggesting th...

journal_title：Nucleic acids research

authors： Kvietikova I,Wenger RH,Marti HH,Gassmann M

更新日期：1995-11-25 00:00:00

abstract：:The Nucleolar Proteome Database (NOPdb) archives data on >700 proteins that were identified by multiple mass spectrometry (MS) analyses from highly purified preparations of human nucleoli, the most prominent nuclear organelle. Each protein entry is annotated with information about its corresponding gene, its domain st...

journal_title：Nucleic acids research

authors： Leung AK,Trinkle-Mulcahy L,Lam YW,Andersen JS,Mann M,Lamond AI

更新日期：2006-01-01 00:00:00

abstract：:We have isolated a human cDNA (kup), encoding a new protein with two distantly spaced zinc fingers of the C2H2 type. This gene is highly conserved in mammals and is expressed mainly in hematopoietic cells and testis. Its expression was not higher in the various transformed cells tested than in the normal corresponding...

journal_title：Nucleic acids research

authors： Chardin P,Courtois G,Mattei MG,Gisselbrecht S

更新日期：1991-04-11 00:00:00

abstract：:The rpoA gene, encoding the alpha subunit of RNA polymerase, was cloned from Streptomyces coelicolor A3(2). It is preceded by rpsK and followed by rplQ, encoding ribosomal proteins S11 and L17, respectively, similar to the gene order in Bacillus subtilis. The rpoA gene specifies a protein of 339 amino acids with deduc...

journal_title：Nucleic acids research

authors： Cho EJ,Bae JB,Kang JG,Roe JH

更新日期：1996-11-15 00:00:00

abstract：:Pfam is a widely used database of protein families, currently containing more than 13,000 manually curated protein families as of release 26.0. Pfam is available via servers in the UK (http://pfam.sanger.ac.uk/), the USA (http://pfam.janelia.org/) and Sweden (http://pfam.sbc.su.se/). Here, we report on changes that ha...

journal_title：Nucleic acids research

authors： Punta M,Coggill PC,Eberhardt RY,Mistry J,Tate J,Boursnell C,Pang N,Forslund K,Ceric G,Clements J,Heger A,Holm L,Sonnhammer EL,Eddy SR,Bateman A,Finn RD

更新日期：2012-01-01 00:00:00

abstract：:The modified nucleoside, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several mammalian tissues, other animals such as starfish, lingula and hagfish, and wheat germ. Q isolated from rat liver tRNA ...

journal_title：Nucleic acids research

authors： Kasai H,Kuchino Y,Nihei K,Nishimura S

更新日期：1975-10-01 00:00:00

abstract：:Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using...

journal_title：Nucleic acids research

authors： Cocco MJ,Hanakahi LA,Huber MD,Maizels N

更新日期：2003-06-01 00:00:00

abstract：:PrimPol is a primase-polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been established. Here, we report that the proposed zinc finger domain ...

journal_title：Nucleic acids research

authors： Keen BA,Jozwiakowski SK,Bailey LJ,Bianchi J,Doherty AJ

更新日期：2014-05-01 00:00:00

abstract：:The nucleotide sequences of eight partial and five full-length phaseolin cDNA clones show that phaseolin polypeptides are encoded by two distinct gene families which differ in their coding regions by the presence or absence of two different size direct repeats. The alpha-type phaseolin polypeptides are encoded by gene...

journal_title：Nucleic acids research

authors： Slightom JL,Drong RF,Klassy RC,Hoffman LM

更新日期：1985-09-25 00:00:00

abstract：:The PR264/SC35 splicing factor belongs to the family of SR proteins which function as essential and alternative splicing factors. Here, we report that the human PR264/SC35 locus is bidirectionally transcribed. Double in situ hybridization experiments have allowed simultaneous detection of sense and antisense RNA in hu...

journal_title：Nucleic acids research

authors： Sureau A,Soret J,Guyon C,Gaillard C,Dumon S,Keller M,Crisanti P,Perbal B

更新日期：1997-11-15 00:00:00

abstract：:VL30 genetic elements constitute a murine multicopy gene family that is retrovirus-like, despite the lack of sequence homology with any known retrovirus. Over one hundred copies of VL30 units are dispersed throughout the mouse genome. We report here that the mouse genome also contains 'solo' VL30 long terminal repeats...

journal_title：Nucleic acids research

authors： Rotman G,Itin A,Keshet E

更新日期：1984-03-12 00:00:00

abstract：:The Mouse Genome Database (MGD) (http://www.informatics.jax.org) one component of a community database resource for the laboratory mouse, a key model organism for interpreting the human genome and for understanding human biology. MGD strives to provide an extensively integrated information resource with experimental d...

journal_title：Nucleic acids research

authors： Blake JA,Richardson JE,Bult CJ,Kadin JA,Eppig JT,Mouse Genome Database Group.

更新日期：2003-01-01 00:00:00

abstract：:The Protein Data Bank in Europe (PDBe, pdbe.org) is actively engaged in the deposition, annotation, remediation, enrichment and dissemination of macromolecular structure data. This paper describes new developments and improvements at PDBe addressing three challenging areas: data enrichment, data dissemination and func...

journal_title：Nucleic acids research

authors： Mir S,Alhroub Y,Anyango S,Armstrong DR,Berrisford JM,Clark AR,Conroy MJ,Dana JM,Deshpande M,Gupta D,Gutmanas A,Haslam P,Mak L,Mukhopadhyay A,Nadzirin N,Paysan-Lafosse T,Sehnal D,Sen S,Smart OS,Varadi M,Kleywegt GJ

更新日期：2018-01-04 00:00:00