Abstract：
:Ribosome-inactivating proteins (RIP) are a family of plant enzymes for which a unique activity was determined: rRNAN-glycosidase at a specific universally conserved position, A4324in the case of rat ribosomes. Recently we have shown that the RIP from Saponaria officinalis have a much wider substrate specificity: they are actually polynucleotide:adenosine glycosidases. Here we extend studies on substrate specificity to most known RIP: 52 purified proteins, both type 1 (single-chain) and type 2 (two chain, an enzymatic chain and a lectin chain) were examined for adenine release on various substrates including RNAs from different sources, DNA, and poly(A). All RIP depurinated extensively DNA and some released adenine from all adenine-containing polynucleotides tested. From experimental evidence the entire class of plant proteins, up to now called ribosome-inactivating proteins, may be classified as polynucleotide:adenosine glycosidases. The newly identified substrates may be implicated in the biological role(s) of RIP.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Barbieri L,Valbonesi P,Bonora E,Gorini P,Bolognesi A,Stirpe Fdoi
10.1093/nar/25.3.518subject
Has Abstractpub_date
1997-02-01 00:00:00pages
518-22issue
3eissn
0305-1048issn
1362-4962pii
gka111journal_volume
25pub_type
杂志文章abstract：:The Rhizobium trifolii nod genes required for host-specific nodulation of clovers are located on 14 kb of Sym (symbiotic) plasmid DNA. Analysis of the nucleotide sequence of a 3.7 kb portion of this region has revealed open reading frames corresponding to the nodABCDEF genes. A DNA sequencing technique, using primer e...
journal_title：Nucleic acids research
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更新日期：1986-04-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.10.3589
更新日期：1978-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1093/nar/28.21.4219
更新日期：2000-11-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki061
更新日期：2005-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.3.679
更新日期：1978-03-01 00:00:00
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journal_title：Nucleic acids research
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journal_title：Nucleic acids research
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更新日期：2009-04-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa442
更新日期：2020-07-09 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2008-10-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2003-01-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.9.3347
更新日期：1978-09-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.102
更新日期：1998-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.19.3779
更新日期：2000-10-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2019-05-07 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1994-04-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh009
更新日期：2004-01-02 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1030
更新日期：2013-01-07 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg417
更新日期：2003-07-15 00:00:00
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更新日期：1992-06-11 00:00:00
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journal_title：Nucleic acids research
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更新日期：2018-09-28 00:00:00
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更新日期：1994-07-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1998-05-01 00:00:00
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更新日期：2006-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1994-08-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr407
更新日期：2011-09-01 00:00:00
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更新日期：2010-11-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2017-10-13 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2017-07-27 00:00:00
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journal_title：Nucleic acids research
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doi：10.1093/nar/19.19.5199
更新日期：1991-10-11 00:00:00
