A recombination based method to rapidly assess specificity of two-hybrid clones in yeast.

abstract：:The carbon catabolite repressor protein 4 (Ccr4)-Negative on TATA (Not) complex controls gene expression at two levels. In the nucleus, it regulates the basal transcription machinery, nuclear receptor-mediated transcription and histone modifications. In the cytoplasm, the complex is required for messenger RNA (mRNA) t...

journal_title：Nucleic acids research

authors： Sandler H,Kreth J,Timmers HT,Stoecklin G

更新日期：2011-05-01 00:00:00

abstract：:For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...

journal_title：Nucleic acids research

authors： Behn M,Schuermann M

更新日期：1998-03-01 00:00:00

abstract：:Calf thymus nucleosomes exhibit two different and independent hydrodynamic responses to diminishing salt concentration. One change is gradual over the range 40 to 0.2 mM Na+ and is accompanied by decreases in contact-site cross-linking efficiency. The other change is abrupt, being centered between 1 and 2 mM Na+. We f...

journal_title：Nucleic acids research

authors： Burch JB,Martinson HG

更新日期：1980-11-11 00:00:00

abstract：:The NONCODE database is an integrated knowledge database designed for the analysis of non-coding RNAs (ncRNAs). Since NONCODE was first released 3 years ago, the number of known ncRNAs has grown rapidly, and there is growing recognition that ncRNAs play important regulatory roles in most organisms. In the updated vers...

journal_title：Nucleic acids research

authors： He S,Liu C,Skogerbø G,Zhao H,Wang J,Liu T,Bai B,Zhao Y,Chen R

更新日期：2008-01-01 00:00:00

abstract：:The cancer drug daunomycin is an effective inhibitor of the B leads to Z transition in poly d(G-C) in 4 M NaCl. Both the rate and extent of the B leads to Z transition are decreased by the drug, as judged by equilibrium and kinetic studies. Daunomycin can, under some conditions, convert Z form DNA back to B form. Drug...

journal_title：Nucleic acids research

authors： Chaires JB

更新日期：1983-12-10 00:00:00

abstract：:The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter...

journal_title：Nucleic acids research

authors： Gérard MA,Myslinski E,Chylak N,Baudrey S,Krol A,Carbon P

更新日期：2010-01-01 00:00:00

abstract：:Recent advances in DNA-sequencing technology have made it possible to obtain large datasets of small RNA sequences. Here we demonstrate that not all non-perfectly matched small RNA sequences are simple technological sequencing errors, but many hold valuable biological information. Analysis of three small RNA datasets ...

journal_title：Nucleic acids research

authors： Ebhardt HA,Tsang HH,Dai DC,Liu Y,Bostan B,Fahlman RP

更新日期：2009-05-01 00:00:00

abstract：:Polyadenylation of nascent transcripts is one of the key mRNA processing events in eukaryotic cells. A large number of human and mouse genes have alternative polyadenylation sites, or poly(A) sites, leading to mRNA variants with different protein products and/or 3'-untranslated regions (3'-UTRs). PolyA_DB 2 contains p...

journal_title：Nucleic acids research

authors： Lee JY,Yeh I,Park JY,Tian B

更新日期：2007-01-01 00:00:00

abstract：:NRPS-PKS is web-based software for analysing large multi-enzymatic, multi-domain megasynthases that are involved in the biosynthesis of pharmaceutically important natural products such as cyclosporin, rifamycin and erythromycin. NRPS-PKS has been developed based on a comprehensive analysis of the sequence and structur...

journal_title：Nucleic acids research

authors： Ansari MZ,Yadav G,Gokhale RS,Mohanty D

更新日期：2004-07-01 00:00:00

abstract：:Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...

journal_title：Nucleic acids research

authors： Kemmerich K,Dingler FA,Rada C,Neuberger MS

更新日期：2012-07-01 00:00:00

abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...

journal_title：Nucleic acids research

authors： Stern DB,Palmer JD

更新日期：1986-07-25 00:00:00

abstract：:Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells ...

journal_title：Nucleic acids research

authors： McLennan AG,Prescott M

更新日期：1984-02-10 00:00:00

abstract：:A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like charac...

journal_title：Nucleic acids research

authors： Simon RH,Felsenfeld G

更新日期：1979-02-01 00:00:00

abstract：:We have determined the DNA sequence surrounding the transcription terminator following rpoC, the gene that codes for the beta' subunit of RNA polymerase in E. coli K12. The 2044 bp sequence obtained contains the distal 335 codons of rpoC followed by a 212 bp non-coding region and a second open reading frame (ORFa) of ...

journal_title：Nucleic acids research

authors： Squires C,Krainer A,Barry G,Shen WF,Squires CL

更新日期：1981-12-21 00:00:00

abstract：:Modification of chromatin and related transcription factors by histone deacetylases (HDACs) is one of the major strategies for controlling gene expression in eukaryotes. The HDAC domains of class IIa HDACs repress the respective target genes by interacting with the C-terminal region of the silencing mediator for retin...

journal_title：Nucleic acids research

authors： Park SY,Kim GS,Hwang HJ,Nam TH,Park HS,Song J,Jang TH,Lee YC,Kim JS

更新日期：2018-12-14 00:00:00

abstract：:Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA s...

journal_title：Nucleic acids research

authors： Roulon T,Coulaud D,Delain E,Le Cam E,Hélène C,Escudé C

更新日期：2002-02-01 00:00:00

abstract：:Here we describe a two-stepped photobiotin-based procedure to enrich a target (canine retinal) cDNA library for tissue specific clones without removing those containing repetitive ( SINE ) elements, despite the presence of these elements in the driver population. In a first hybridization excess SINE elements were hybr...

journal_title：Nucleic acids research

authors： Lin CT,Sargan DR

更新日期：1997-11-01 00:00:00

abstract：:It has been proposed that phage lambda site-specific recombination proceeds via two independent strand exchanges: the first exchange forming a Holliday-structure which is then converted into complete recombinant products by the second strand exchange. If this hypothesis is correct, one should be able to trap the putat...

journal_title：Nucleic acids research

authors： Kitts PA,Nash HA

更新日期：1988-07-25 00:00:00

abstract：:Aberrant CpG methylation changes occurring during tumour progression include the loss (hypomethylation) and gain (hypermethylation) of methyl groups. Techniques currently available for examining such changes either require selection of a region, then examination of methylation changes, or utilise methylation-sensitive...

journal_title：Nucleic acids research

authors： Brock GJ,Huang TH,Chen CM,Johnson KJ

更新日期：2001-12-15 00:00:00

abstract：:Structural Maintenance of Chromosomes (SMC) proteins are vital for a wide range of processes including chromosome structure and dynamics, gene regulation and DNA repair. Eukaryotes have three SMC complexes, consisting of heterodimeric pairs of six different SMC proteins along with several specific regulatory subunits....

journal_title：Nucleic acids research

authors： Griese JJ,Witte G,Hopfner KP

更新日期：2010-06-01 00:00:00

abstract：:Interleukin 2 (IL2) mRNA has a short half-life in the cytoplasm of T lymphocytes, relative to most mRNA. We have discovered a candidate ribonuclease to account for the rapid turnover of IL2 mRNA in the cytosol of the human T lymphocyte cell line Jurkat. In partially purified form, this RNase is about 7 times as active...

journal_title：Nucleic acids research

authors： Hua J,Garner R,Paetkau V

更新日期：1993-01-11 00:00:00

abstract：:RNA fragments from phage fr covering the complete or part of the replicase cistron initiation region have been used as templates in the formation of a ribosomal initiation complex in vitro. The results so obtained together with our earlier findings in a similar approach applied to fragments of the structurally related...

journal_title：Nucleic acids research

authors： Berzin V,Cielens I,Jansone I,Gren EJ

更新日期：1982-12-11 00:00:00

abstract：:Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn2+ which cannot be replaced by Mg2+ or Ca2+, and makes single-strand scissions in double-stranded DNA. Endonculeas...

journal_title：Nucleic acids research

authors： Pinon R,Leney E

更新日期：1975-07-01 00:00:00

abstract：:Termination of protein synthesis is promoted in ribosomes by proper stop codon discrimination by class 1 polypeptide release factors (RFs). A large set of prokaryotic RFs differing in stop codon specificity, RF1 for UAG and UAA, and RF2 for UGA and UAA, was analyzed by means of a recently developed computational metho...

journal_title：Nucleic acids research

authors： Oparina NJ,Kalinina OV,Gelfand MS,Kisselev LL

更新日期：2005-09-14 00:00:00

abstract：:A complete nucleotide sequence of human aldolase B mRNA was determined with a recombinant cDNA (pHABL120-3). The cDNA insert was composed of 1,652 bases excluding poly(A) tail and the sequence was consistent with the previous results reported by others. However, S1 nuclease mapping and subsequent genomic analysis allo...

journal_title：Nucleic acids research

authors： Sakakibara M,Mukai T,Yatsuki H,Hori K

更新日期：1985-07-25 00:00:00

abstract：:Three protein isoforms (74, 54 and 42 kDa) are expressed from repressor gene c in the Streptomyces temperate bacteriophage phiC31. Because expression of the two smaller isoforms, 54 and 42 kDa, is sufficient for superinfection immunity, the interaction between these isoforms was studied. The native 42 kDa repressor (N...

journal_title：Nucleic acids research

authors： Wilson SE,Smith MC

更新日期：1998-05-15 00:00:00

abstract：:The history and mechanism of molecular evolution in DNA have been greatly elucidated by contributions from genetics, probability theory and bioinformatics--indeed, mathematical developments such as Kimura's neutral theory, Kingman's coalescent theory and efficient software such as BLAST, ClustalW, Phylip, etc., provid...

journal_title：Nucleic acids research

authors： Waldispühl J,Devadas S,Berger B,Clote P

更新日期：2009-07-01 00:00:00

abstract：:TcruziDB (http://TcruziDB.org) is an integrated genome database for the parasitic organism Trypanosoma cruzi, the causative agent of Chagas' disease. The database currently incorporates all available sequence data (Genomic, BAC, EST) in a single user-friendly location. The database contains a variety of tools specific...

journal_title：Nucleic acids research

authors： Luchtan M,Warade C,Weatherly DB,Degrave WM,Tarleton RL,Kissinger JC

更新日期：2004-01-01 00:00:00

abstract：:The complete nucleotide sequence of the coding region of foot and mouth disease virus RNA (strain A1061) is presented. The sequence extends from the primary initiation site, approximately 1200 nucleotide from the 5' end of the genome, in an open translational reading frame of 6,999 nucleotides to a termination codon 9...

journal_title：Nucleic acids research

authors： Carroll AR,Rowlands DJ,Clarke BE

更新日期：1984-03-12 00:00:00

abstract：:We have compiled the DNA sequence data for E. coli K12 available from the GENBANK and EMBO databases and over a period of several years independently from the literature. We have introduced all available genetic map data and have arranged the sequences accordingly. As far as possible the overlaps are deleted and a tot...

journal_title：Nucleic acids research

authors： Kröger M

更新日期：1989-01-01 00:00:00