COBRA: a sensitive and quantitative DNA methylation assay.

Abstract:

:We report here on a quantitative technique called COBRA to determine DNA methylation levels at specific gene loci in small amounts of genomic DNA. Restriction enzyme digestion is used to reveal methylation-dependent sequence differences in PCR products of sodium bisulfite-treated DNA as described previously. We show that methylation levels in the original DNA sample are represented by the relative amounts of digested and undigested PCR product in a linearly quantitative fashion across a wide spectrum of DNA methylation levels. In addition, we show that this technique can be reliably applied to DNA obtained from microdissected paraffin-embedded tissue samples. COBRA thus combines the powerful features of ease of use, quantitative accuracy, and compatibility with paraffin sections.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Xiong Z,Laird PW

doi

10.1093/nar/25.12.2532

subject

Has Abstract

pub_date

1997-06-15 00:00:00

pages

2532-4

issue

12

eissn

0305-1048

issn

1362-4962

pii

gka376

journal_volume

25

pub_type

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