Abstract:
:Two triple helix structures (15-mers containing only T.A-T triplets or containing mixed T.A-T and C.G-C triplets) have been studied by uranyl mediated DNA photocleavage to probe the accessibility of the phosphates of the DNA backbone. Whereas the phosphates of the pyrimidine strand are at least as accessible as in double stranded DNA, in the phosphates of the purine strand are partly shielded and more so at the 5'-end of the strand. With the homo A/T target increased cleavage is observed towards the 3'-end on the pyrimidine strand. These results show that the third strand is asymmetrically positioned along the groove with the tightest triple strand double strand interactions at the 5'-end of the third strand. The results also indicate that homo-A versus mixed A/G 'Hoogsteen-triple helices' have different structures.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nielsen PEdoi
10.1093/nar/20.11.2735keywords:
subject
Has Abstractpub_date
1992-06-11 00:00:00pages
2735-9issue
11eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract::CTCF plays a pivotal role in mediating chromatin interactions, but it does not do so alone. A number of factors have been reported to co-localize with CTCF and regulate CTCF loops, but no comprehensive analysis of binding partners has been performed. This prompted us to identify CTCF loop participants and regulators b...
journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1979-06-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky656
更新日期:2018-10-12 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.10.3177
更新日期:1979-07-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1979-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1200
更新日期:2014-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn414
更新日期:2008-08-01 00:00:00
abstract::The three dimensional crystal structure of T5 5'-3' exonuclease was compared with that of two other members of the 5'-3' exonuclease family: T4 ribonuclease H and the N-terminal domain of Thermus aquaticus DNA polymerase I. Though these structures were largely similar, some regions of these enzymes show evidence of si...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.21.4224
更新日期:1997-11-01 00:00:00
abstract::Conserved in a variety of evolutionarily divergent plant species, LOB DOMAIN (LBD) genes define a large, plant-specific family of largely unknown function. LBD genes have been implicated in a variety of developmental processes in plants, although to date, relatively few members have been assigned functions. LBD protei...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm775
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks822
更新日期:2012-11-01 00:00:00
abstract::Escherichia coli has long been regarded as a model organism in the study of codon usage bias (CUB). However, most studies in this organism regarding this topic have been computational or, when experimental, restricted to small datasets; particularly poor attention has been given to genes with low CUB. In this work, co...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg897
更新日期:2003-12-01 00:00:00
abstract::Quantitative real-time PCR has revolutionized many aspects of genetic research, biomedical diagnostics and pathogen detection. Nevertheless, the full potential of this technology has yet to be realized, primarily due to the limitations of the threshold-based methodologies that are currently used for quantitative analy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh177
更新日期:2004-12-15 00:00:00
abstract::The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv870
更新日期:2015-12-15 00:00:00
abstract::X-linked agammaglobulinemia (XLA) is an immunodeficiency caused by mutations in the gene coding for Bruton's agammaglobulinemia tyrosine kinase (BTK). A database (BTKbase) of BTK mutations has been compiled and the recent update lists 368 entries from 318 unrelated families showing 228 unique molecular events. In addi...
journal_title:Nucleic acids research
pub_type: 杂志文章,多中心研究
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更新日期:1997-01-01 00:00:00
abstract::The plant gene enod40 is highly conserved among legumes and also present in various non-legume species. It is presumed to play a central regulatory role in the Rhizobium-legume interaction, being expressed well before the initiation of cortical cell divisions resulting in nodule formation. Two small peptides encoded b...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1979-10-10 00:00:00
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journal_title:Nucleic acids research
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更新日期:2017-07-03 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-01-24 00:00:00
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journal_title:Nucleic acids research
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更新日期:2009-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1985-02-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-11-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2000-04-15 00:00:00
abstract::Genome-wide association studies often incorporate information from public biological databases in order to provide a biological reference for interpreting the results. The dbSNP database is an extensive source of information on single nucleotide polymorphisms (SNPs) for many different organisms, including humans. We h...
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更新日期:2011-01-01 00:00:00