Endonucleolytic activation directs dark-induced chloroplast mRNA degradation.

Abstract:

:Plastid mRNA stability is tightly regulated by external signals such as light. We have investigated the biochemical mechanism responsible for the dark-induced decrease of relative half-lives for mRNAs encoding photosynthetic proteins. Protein fractions isolated from plastids of light-grown and dark-adapted plants correctly reproduced an RNA degradation pathway in the dark that is downregulated in the light. This dark-dependent pathway is initiated by endonucleolytic cleavages in the petD mRNA precursor substrate proximal to a region that can fold into a stem-loop structure. Polynucleotide phosphorylase (PNPase) polyadenylation activity was strongly increased in the protein fraction isolated from plastids in dark-adapted plants, but interestingly PNPase activity was not required for the initiation of dark-induced mRNA degradation. A protein factor present in the protein fraction from plastids of light-grown plants could inactivate the endonuclease activity and thereby stabilize the RNA substrate in the protein fraction from plastids of dark-adapted plants. The results show that plastid mRNA stability is effectively controlled by the regulation of a specific dark-induced RNA degradation pathway.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Baginsky S,Gruissem W

doi

10.1093/nar/gkf561

keywords:

subject

Has Abstract

pub_date

2002-10-15 00:00:00

pages

4527-33

issue

20

eissn

0305-1048

issn

1362-4962

journal_volume

30

pub_type

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