A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop.

abstract：:Bleomycin B(2)(BLM) in the presence of iron [Fe(II)] and O(2)catalyzes single-stranded (ss) and double-stranded (ds) cleavage of DNA. Electrospray ionization ion trap mass spectrometry was used to monitor these cleavage processes. Two duplex oligonucleotides containing an ethylene oxide tether between both strands wer...

journal_title：Nucleic acids research

authors： Harsch A,Marzilli LA,Bunt RC,Stubbe J,Vouros P

更新日期：2000-05-01 00:00:00

abstract：:We examined the ability of the 5' flanking region sequences of a human U1 RNA gene to direct synthesis of functional mRNA. When fused to chloramphenicol acetyltransferase (CAT) coding region sequences, the upstream sequences of the U1 gene were able to stimulate the synthesis of functional CAT mRNA in 293 cells but no...

journal_title：Nucleic acids research

authors： Dahlberg JE,Schenborn ET

更新日期：1988-07-11 00:00:00

abstract：:Assessment of the fidelity of gene expression is crucial to understand cell homeostasis. Here we present a highly sensitive method for the systematic Quantification of Rare Amino acid Substitutions (QRAS) using absolute quantification by targeted mass spectrometry after chromatographic enrichment of peptides with miss...

journal_title：Nucleic acids research

authors： Garofalo R,Wohlgemuth I,Pearson M,Lenz C,Urlaub H,Rodnina MV

更新日期：2019-04-08 00:00:00

abstract：:We have generated a codon usage table for Pseudomonas aeruginosa. Codon usage in P. aeruginosa is extremely biased. In contrast to E. coli and yeast, P. aeruginosa preferentially uses those codons within a synonymous codon group with the strongest predicted codon-anticodon interaction. We were unable to correlate a pa...

journal_title：Nucleic acids research

authors： West SE,Iglewski BH

更新日期：1988-10-11 00:00:00

abstract：:The gene, rpb2, encoding the second largest subunit, subunit 2, of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB2, of Saccharomyces cerevisiae as a probe for cross-hybridization. We have determined the complete nucleotide sequence of rpb2, and parts of the PCR-ampli...

journal_title：Nucleic acids research

authors： Kawagishi M,Yamagishi M,Ishihama A

更新日期：1993-02-11 00:00:00

abstract：:RNA-based therapeutics, i.e. the utilization of synthetic RNA molecules to alter cellular functions, have the potential to address targets which are currently out of scope for traditional drug design pipelines. This potential however hinges on the ability to selectively deliver and internalize therapeutic RNAs into ce...

journal_title：Nucleic acids research

authors： Wang Y,Hoinka J,Liang Y,Adamus T,Swiderski P,Przytycka TM

更新日期：2018-09-19 00:00:00

abstract：:In the known monoclinic crystals the 3-dimensional structure of the hexameric, replicative helicase RepA encoded by plasmid RSF1010 shows 6-fold rotational symmetry. In contrast, in the cubic crystal form at 2.55 A resolution described here RepA has 3-fold symmetry and consists of a trimer of dimers. To study structur...

journal_title：Nucleic acids research

authors： Ziegelin G,Niedenzu T,Lurz R,Saenger W,Lanka E

更新日期：2003-10-15 00:00:00

abstract：:Interaction detection methods have led to the discovery of thousands of interactions between proteins, and discerning relevance within large-scale data sets is important to present-day biology. Here, a spectral method derived from graph theory was introduced to uncover hidden topological structures (i.e. quasi-cliques...

journal_title：Nucleic acids research

authors： Bu D,Zhao Y,Cai L,Xue H,Zhu X,Lu H,Zhang J,Sun S,Ling L,Zhang N,Li G,Chen R

更新日期：2003-05-01 00:00:00

abstract：:Ribosomal protein L4 of Escherichia coli regulates expression of its own eleven gene S10 operon both by inhibiting translation and by stimulating premature termination of transcription. Both regulatory processes presumably involve L4 recognition of the S10 leader RNA. To help define L4's regulatory target, we have inv...

journal_title：Nucleic acids research

authors： Zengel JM,Lindahl L

更新日期：1993-05-25 00:00:00

abstract：:Purified 15 S globin mRNA-protein (mRNP) complexes obtained by EDTA dissociation of duck reticulocytes polyribosomes were digested with the calcium dependant Staphylococcus aureus nuclease (EC 3. 1. 4. 7.). 25% of the globin mRNA sequences were resistant to extensive nuclease digestion as determined by TCA precipitati...

journal_title：Nucleic acids research

authors： Goldenberg S,Vincent A,Scherrer K

更新日期：1979-06-25 00:00:00

abstract：:The methyltransferase, M.EcoKI, recognizes the DNA sequence 5'-AACNNNNNNGTGC-3' and methylates adenine at the underlined positions. DNA methylation has been shown by crystallography to occur via a base flipping mechanism and is believed to be a general mechanism for all methyltransferases. If no structure is available...

journal_title：Nucleic acids research

authors： Su TJ,Connolly BA,Darlington C,Mallin R,Dryden DT

更新日期：2004-04-23 00:00:00

abstract：:Cells deficient in the Werner syndrome protein (WRN) or BRCA1 are hypersensitive to DNA interstrand cross-links (ICLs), whose repair requires nucleotide excision repair (NER) and homologous recombination (HR). However, the roles of WRN and BRCA1 in the repair of DNA ICLs are not understood and the molecular mechanisms...

journal_title：Nucleic acids research

authors： Cheng WH,Kusumoto R,Opresko PL,Sui X,Huang S,Nicolette ML,Paull TT,Campisi J,Seidman M,Bohr VA

更新日期：2006-05-19 00:00:00

abstract：:Granulocyte macrophage-colony stimulating factor (GM-CSF) is produced by T cells, but not B cells, in response to immune signals. GM-CSF gene activation in response to T-cell stimulation requires remodelling of chromatin associated with the gene promoter, and these changes do not occur in B cells. While the CpG methyl...

journal_title：Nucleic acids research

authors： Brettingham-Moore KH,Sprod OR,Chen X,Oakford P,Shannon MF,Holloway AF

更新日期：2008-05-01 00:00:00

abstract：:The synthesis of 1-(beta-D-2'-deoxyribosyl)-2-pyrimidinone (dK) and its 5-methyl derivative (d5) from 2'-deoxycytidine or 2'-deoxythymidine, respectively, via silver oxide oxidation of 4-hydrazinopyrimidines is described. The necessary hydrazine substituted pyrimidine nucleosides have been prepared by transamination o...

journal_title：Nucleic acids research

authors： Gildea B,McLaughlin LW

更新日期：1989-03-25 00:00:00

abstract：:The adenovirus late region 1 (L1) represents an example of an alternatively spliced gene where one 5' splice site is spliced to two alternative 3' splice sites, to produce two mRNAs; the 52,55K and IIIa mRNAs, respectively. Accumulation of the L1 mRNAs is temporally regulated during the infectious cycle. Thus, the pro...

journal_title：Nucleic acids research

authors： Kreivi JP,Akusjärvi G

更新日期：1994-02-11 00:00:00

abstract：:Interferons (IFN) play a pivotal role in innate immunity, orchestrating a cell-intrinsic anti-pathogenic state and stimulating adaptive immune responses. The complex interplay between the primary response to IFNs and its modulation by positive and negative feedback loops is incompletely understood. Here, we implement ...

journal_title：Nucleic acids research

authors： Trilling M,Bellora N,Rutkowski AJ,de Graaf M,Dickinson P,Robertson K,Prazeres da Costa O,Ghazal P,Friedel CC,Albà MM,Dölken L

更新日期：2013-09-01 00:00:00

abstract：:The temperature-sensitive Neurospora nuclear mutant cyt18-1 is deficient in splicing many Group I mitochondrial introns when grown at its non-permissive temperature; however, splicing of intron 1 in the coI gene of the Adiopodoume (formerly called North Africa) strain is unaffected (R.A. Collins and A.M. Lambowitz, J....

journal_title：Nucleic acids research

authors： Field DJ,Sommerfield A,Saville BJ,Collins RA

更新日期：1989-11-25 00:00:00

abstract：:MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experi...

journal_title：Nucleic acids research

authors： Leclercq M,Diallo AB,Blanchette M

更新日期：2017-01-25 00:00:00

abstract：:The widespread applications of various 'omics' technologies in biomedical research together with the emergence of public data repositories have resulted in a plethora of data sets for almost any given physiological state or disease condition. Properly combining or integrating these data sets with similar basic hypothe...

journal_title：Nucleic acids research

authors： Xia J,Fjell CD,Mayer ML,Pena OM,Wishart DS,Hancock RE

更新日期：2013-07-01 00:00:00

abstract：:ATR functions as a master regulator of the DNA-damage response. ATR activation requires the ATR activator, topoisomerase IIβ-binding protein 1 (TopBP1). However, the underlying mechanism of TopBP1 regulation and how its regulation affects DNA replication remain unknown. Here, we report a specific interaction between T...

journal_title：Nucleic acids research

authors： Feng H,Lu J,Song X,Thongkum A,Zhang F,Lou L,Reizes O,Almasan A,Gong Z

更新日期：2020-11-04 00:00:00

abstract：:In this study, we demonstrate that the lack of retinoic acid-related orphan receptor (ROR) γ or α expression in mice significantly reduced the peak expression level of Cry1, Bmal1, E4bp4, Rev-Erbα and Per2 in an ROR isotype- and tissue-selective manner without affecting the phase of their rhythmic expression. Analysis...

journal_title：Nucleic acids research

authors： Takeda Y,Jothi R,Birault V,Jetten AM

更新日期：2012-09-01 00:00:00

abstract：:The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variabl...

journal_title：Nucleic acids research

authors： Cattaneo R,Gorski J,Mach B

更新日期：1981-06-25 00:00:00

abstract：:R.E.D. Server is a unique, open web service, designed to derive non-polarizable RESP and ESP charges and to build force field libraries for new molecules/molecular fragments. It provides to computational biologists the means to derive rigorously molecular electrostatic potential-based charges embedded in force field l...

journal_title：Nucleic acids research

authors： Vanquelef E,Simon S,Marquant G,Garcia E,Klimerak G,Delepine JC,Cieplak P,Dupradeau FY

更新日期：2011-07-01 00:00:00

abstract：:PubChem (https://pubchem.ncbi.nlm.nih.gov) is a popular chemical information resource that serves the scientific community as well as the general public, with millions of unique users per month. In the past two years, PubChem made substantial improvements. Data from more than 100 new data sources were added to PubChem...

journal_title：Nucleic acids research

authors： Kim S,Chen J,Cheng T,Gindulyte A,He J,He S,Li Q,Shoemaker BA,Thiessen PA,Yu B,Zaslavsky L,Zhang J,Bolton EE

更新日期：2021-01-08 00:00:00

abstract：:A dot hybridization method is presented for rapidly determining the relative concentrations of nucleic acids in a mixture, as well as the extent of sequence homology between related RNA or DNA species. ...

journal_title：Nucleic acids research

authors： Kafatos FC,Jones CW,Efstratiadis A

更新日期：1979-11-24 00:00:00

abstract：:We describe a versatile and extensible integrated bioinformatics toolkit for the analysis of biological sequences over the Internet. The web portal offers convenient interactive access to a growing pool of chainable bioinformatics software tools and databases that are centrally installed and maintained by the RZG. Cur...

journal_title：Nucleic acids research

authors： Rampp M,Soddemann T,Lederer H

更新日期：2006-07-01 00:00:00

abstract：:Recent effective use of TAL Effectors (TALEs) has provided an important approach to the design and synthesis of sequence-specific DNA-binding proteins. However, it is still a challenging task to design and manufacture effective TALE modulators because of the limited knowledge of TALE-DNA interactions. Here we synthesi...

journal_title：Nucleic acids research

authors： Zhang H,Li J,Hou S,Wang G,Jiang M,Sun C,Hu X,Zhuang F,Dai Z,Dai J,Xi JJ

更新日期：2014-08-01 00:00:00

abstract：:Conserved segments in DNA or protein sequences are strong candidates for functional elements and thus appropriate methods for computing them need to be developed and compared. We describe five methods and computer programs for finding highly conserved blocks within previously computed multiple alignments, primarily fo...

journal_title：Nucleic acids research

authors： Stojanovic N,Florea L,Riemer C,Gumucio D,Slightom J,Goodman M,Miller W,Hardison R

更新日期：1999-10-01 00:00:00

abstract：:The alcohol dehydrogenase (Adh) gene of D. melanogaster is transcribed from two different promoters during fly development: the distal (adult) and the proximal (embryonic-larval). Certain aspects of Adh gene regulation are represented in Drosophila continuous cell lines. We have used Drosophila tissue culture cells in...

journal_title：Nucleic acids research

authors： Benyajati C,Ayer S,McKeon J,Ewel A,Huang J

更新日期：1987-10-12 00:00:00

abstract：:Here we report proof-of-principle for a microsphere-based genotyping assay that detects single nucleotide polymorphisms (SNPs) directly from human genomic DNA samples. This assay is based on a structure-specific cleavage reaction that achieves single base discrimination with a 5'-nuclease which recognizes a tripartite...

journal_title：Nucleic acids research

authors： Rao KV,Stevens PW,Hall JG,Lyamichev V,Neri BP,Kelso DM

更新日期：2003-06-01 00:00:00