Abstract:
:The ability of Deinococcus radiodurans to recover from extensive DNA damage is due in part to its ability to efficiently repair its genome, even following severe fragmentation by hundreds of double-strand breaks. The single-strand annealing pathway plays an important role early during the recovery process, making use of a protein, DdrB, shown to greatly stimulate ssDNA annealing. Here, we report the structure of DdrB bound to ssDNA to 2.3 Å. Pentameric DdrB was found to assemble into higher-order structures that coat ssDNA. To gain further mechanistic insight into the protein's function, a number of point mutants were generated altering both DNA binding and higher order oligomerization. This work not only identifies higher-order DdrB associations but also suggests the presence of an extended DNA binding surface running along the 'top' surface of a DdrB pentamer and continuing down between two individual subunits of the ring structure. Together this work sheds new insight into possible mechanisms for DdrB function in which higher-order assemblies of DdrB pentamers assist in the pairing of complementary ssDNA using an extended DNA binding surface.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sugiman-Marangos SN,Peel JK,Weiss YM,Ghirlando R,Junop MSdoi
10.1093/nar/gkt759subject
Has Abstractpub_date
2013-11-01 00:00:00pages
9934-44issue
21eissn
0305-1048issn
1362-4962pii
gkt759journal_volume
41pub_type
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