Cell cycle regulated synthesis of an abundant transcript for human chromosomal protein HMG-17.

abstract：:African trypanosomes are extracellular protozoan parasites that are transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); the tsetse fly (procyclic) forms express instead the procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in pr...

journal_title：Nucleic acids research

authors： Biebinger S,Rettenmaier S,Flaspohler J,Hartmann C,Peña-Diaz J,Wirtz LE,Hotz HR,Barry JD,Clayton C

更新日期：1996-04-01 00:00:00

abstract：:In Escherichia coli transcription of individual genes generally requires concomitant translation, and thus the decay of mRNAs cannot be studied without the complication of translation. Here we have used T7 RNA polymerase to transcribe in vivo lacZ genes carrying ribosome binding sites of variable efficiency. We show t...

journal_title：Nucleic acids research

authors： Chevrier-Miller M,Jacques N,Raibaud O,Dreyfus M

更新日期：1990-10-11 00:00:00

abstract：:Gene trap vectors developed for genome-wide mutagenesis can be used to study factors governing the expression of exons inserted throughout the genome. For example, entrapment vectors consisting of a partial 3'-terminal exon [i.e. a neomycin resistance gene (Neo), a poly(A) site, but no 3' splice site] were typically e...

journal_title：Nucleic acids research

authors： Osipovich AB,White-Grindley EK,Hicks GG,Roshon MJ,Shaffer C,Moore JH,Ruley HE

更新日期：2004-05-20 00:00:00

abstract：:The "ovalbumin Y" gene, one of three which constitute the ovalbumin gene family in chicken has been completely sequenced. The exact location of exons can be derived from the comparison with the ovalbumin gene sequence and from the map previously established by electron microscopy analysis. During evolution of the Y ge...

journal_title：Nucleic acids research

authors： Heilig R,Muraskowsky R,Kloepfer C,Mandel JL

更新日期：1982-07-24 00:00:00

abstract：:Expansions of (GAA)n repeats within the first intron of the frataxin gene reduce its expression, resulting in a hereditary neurodegenerative disorder, Friedreich's ataxia. While it is generally believed that expanded (GAA)n repeats block transcription elongation, fine mechanisms responsible for gene repression are not...

journal_title：Nucleic acids research

authors： Krasilnikova MM,Kireeva ML,Petrovic V,Knijnikova N,Kashlev M,Mirkin SM

更新日期：2007-01-01 00:00:00

abstract：:Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct complexes with 5S ribosomal RNA in Xenopus oocytes. Additionally, TFIIIA binds the internal promoter of the 5S RNA gene and supports assembly of a transcription initiation complex. Both proteins have nine tandemly repeated zinc fingers with a...

journal_title：Nucleic acids research

authors： Ryan RF,Darby MK

更新日期：1998-02-01 00:00:00

abstract：:The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a suite of database resources to support worldwide research activities in both academia and industry. With the explosive growth of multi-omics data, CNCB-NGDC is continually expanding, updating and enriching...

journal_title：Nucleic acids research

authors： CNCB-NGDC Members and Partners.

更新日期：2021-01-08 00:00:00

abstract：:Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide s...

journal_title：Nucleic acids research

authors： Groot GS,van Harten-Loosbroek N,van Ommen GJ,Pijst HL

更新日期：1981-12-11 00:00:00

abstract：:With the recent increase in the number of mammalian genomes being sequenced, large-scale genome scans for human-specific positive selection are now possible. Selection can be inferred through phylogenetic analysis by comparing the rates of silent and replacement substitution between related species. Maximum-likelihood...

journal_title：Nucleic acids research

authors： Nickel GC,Tefft D,Adams MD

更新日期：2008-01-01 00:00:00

abstract：:We have previously investigated the role of the N-terminal region of ribosomal protein S4 to participate in 30S ribosome assembly and function (1-3). In this report we extend these studies to the two fragments produced by the chemical cleavage of protein S4 at the tryptophan residue 167. We find that the carboxyl term...

journal_title：Nucleic acids research

authors： Changchien LM,Craven GR

更新日期：1985-09-11 00:00:00

abstract：:Analysis of large-scale gene expression studies usually begins with gene clustering. A ubiquitous problem is that different algorithms applied to the same data inevitably give different results, and the differences are often substantial, involving a quarter or more of the genes analyzed. This raises a series of import...

journal_title：Nucleic acids research

authors： Hart CE,Sharenbroich L,Bornstein BJ,Trout D,King B,Mjolsness E,Wold BJ

更新日期：2005-05-10 00:00:00

abstract：:Preparations of chicken, rat and human nuclear 5S RNA contain two sets of molecules. The set with the lowest electrophoretic mobility (5Sa) contains RNAs identical or closely related to ribosomal 5S RNA from the corresponding animal species. In HeLa cells and rat brain, we only detected an RNA identical to the ribosom...

journal_title：Nucleic acids research

authors： Krol A,Gallinaro H,Lazar E,Jacob M,Branlant C

更新日期：1981-02-25 00:00:00

abstract：:Nucleobase analogs 5-methylisocytosine ((Me)isoC) and isoguanine (isoG) form a non-natural base pair in duplex nucleic acids with base pairing specificity orthogonal to the natural nucleobase pairs. Sequencing reactions were conducted with oligodeoxyribonucleotides (ODNs) containing d(Me)isoC and disoG using modified ...

journal_title：Nucleic acids research

authors： Ahle JD,Barr S,Chin AM,Battersby TR

更新日期：2005-06-02 00:00:00

abstract：:PCAF and hGCN5 are distinct human genes that encode proteins related to the yeast histone acetyltransferase and transcriptional adapter GCN5. The PCAF protein shares extensive similarity with the 439 amino acids of yGCN5, but it has an approximately 350 amino acid N-terminal extension that interacts with the transcrip...

journal_title：Nucleic acids research

authors： Smith ER,Belote JM,Schiltz RL,Yang XJ,Moore PA,Berger SL,Nakatani Y,Allis CD

更新日期：1998-06-15 00:00:00

abstract：:We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Repl...

journal_title：Nucleic acids research

authors： Edenberg HJ,Waqar MA,Huberman JA

更新日期：1977-09-01 00:00:00

abstract：:The transcriptional activity of the p53 tumor suppressor protein is crucial for the regulation of cell growth, apoptosis and tumor progression. The first identified p53 relative, p73, was reported to be monoallelically expressed in normal tissues. In some tumors, loss of heterozygosity was associated with overexpressi...

journal_title：Nucleic acids research

authors： Vikhanskaya F,D'Incalci M,Broggini M

更新日期：2000-01-15 00:00:00

abstract：:A new thermodynamic database for normal and modified nucleic acids has been developed. This Thermodynamic Database for Nucleic Acids (NTDB) includes sequence, structure and thermodynamic information as well as experimental methods and conditions. In this release, there are 1851 sequences containing both normal and mod...

journal_title：Nucleic acids research

authors： Chiu WL,Sze CN,Ip LN,Chan SK,Au-Yeung SC

更新日期：2001-01-01 00:00:00

abstract：:Natural antisense transcripts are at least partially complementary to their sense transcripts. Cis-Sense/Antisense pairs (cis-SAs) have been extensively characterized and known to play diverse regulatory roles, whereas trans-Sense/Antisense pairs (trans-SAs) in animals are poorly studied. We identified long trans-SAs ...

journal_title：Nucleic acids research

authors： Li JT,Zhang Y,Kong L,Liu QR,Wei L

更新日期：2008-09-01 00:00:00

abstract：:Pathogenic bacteria encounter host-imposed manganese (Mn) limitation during infection. Herein we report that in the human pathogen Streptococcus pyogenes, the adaptive response to Mn limitation is controlled by a DtxR family metalloregulator, MtsR. Genes upregulated by MtsR during Mn limitation include Mn (mtsABC) and...

journal_title：Nucleic acids research

authors： Do H,Makthal N,Chandrangsu P,Olsen RJ,Helmann JD,Musser JM,Kumaraswami M

更新日期：2019-08-22 00:00:00

abstract：:Lens epithelium-derived growth factor/p75 (LEDGF/p75) is a transcriptional coactivator involved in stress response, autoimmune disease, cancer and HIV replication. A fusion between the nuclear pore protein NUP98 and LEDGF/p75 has been found in human acute and chronic myeloid leukemia and association of LEDGF/p75 with ...

journal_title：Nucleic acids research

authors： De Rijck J,Bartholomeeusen K,Ceulemans H,Debyser Z,Gijsbers R

更新日期：2010-10-01 00:00:00

abstract：:GC-AG introns represent 0.7% of total human pre-mRNA introns. To study the function of GC-AG introns in splicing regulation, 196 cDNA-confirmed GC-AG introns were identified in Caenorhabditis elegans. These represent 0.6% of the cDNA- confirmed intron data set for this organism. Eleven of these GC-AG introns are invol...

journal_title：Nucleic acids research

authors： Farrer T,Roller AB,Kent WJ,Zahler AM

更新日期：2002-08-01 00:00:00

abstract：:Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...

journal_title：Nucleic acids research

authors： Kemmerich K,Dingler FA,Rada C,Neuberger MS

更新日期：2012-07-01 00:00:00

abstract：:DNA swivel (nicking-rejoining) enzyme activity has been studied in various cell fractions of a human lymphoid cell line. Swivel activity is found only in chromatin and in a nuclear membrane fraction containing DNA and possessing endogenous DNA synthesizing activity. Twenty percent of the total swivel activity and less...

journal_title：Nucleic acids research

authors： Yoshida S,Ungers G,Rosenberg BH

更新日期：1977-01-01 00:00:00

abstract：:TIGRFAMs is a collection of protein family definitions built to aid in high-throughput annotation of specific protein functions. Each family is based on a hidden Markov model (HMM), where both cutoff scores and membership in the seed alignment are chosen so that the HMMs can classify numerous proteins according to the...

journal_title：Nucleic acids research

authors： Selengut JD,Haft DH,Davidsen T,Ganapathy A,Gwinn-Giglio M,Nelson WC,Richter AR,White O

更新日期：2007-01-01 00:00:00

abstract：:The thymine analog 5-chlorouridine, first reported in the 1950s as anti-tumor agent, is known as an effective mutagen, clastogen and toxicant as well as an effective inducer of sister-chromatid exchange. Recently, the first microorganism with a chemically different genome was reported; the selected Escherichia coli st...

journal_title：Nucleic acids research

authors： Patra A,Harp J,Pallan PS,Zhao L,Abramov M,Herdewijn P,Egli M

更新日期：2013-02-01 00:00:00

abstract：:Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a bio...

journal_title：Nucleic acids research

authors： Vazquez-Anderson J,Mihailovic MK,Baldridge KC,Reyes KG,Haning K,Cho SH,Amador P,Powell WB,Contreras LM

更新日期：2017-05-19 00:00:00

abstract：:In a recent paper we have estimated the total number of protein coding open reading frames (ORFs) in the Saccharomyces cerevisiae genome, based on their properties, at about 4800. This number is much smaller than the 5800-6000 which is widely accepted. In this paper we analyse differences between the set of ORFs with ...

journal_title：Nucleic acids research

authors： Mackiewicz P,Kowalczuk M,Gierlik A,Dudek MR,Cebrat S

更新日期：1999-09-01 00:00:00

abstract：:Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the cytoplasmic mRNA population during development of fertilized female gametes of the unicellular malaria parasite, Plasmo...

journal_title：Nucleic acids research

authors： Braks JA,Mair GR,Franke-Fayard B,Janse CJ,Waters AP

更新日期：2008-03-01 00:00:00

abstract：:A cross index is presented for using the improved selectivity offered by the Hung and Wensink (Nucl. Acids Res. 12, 1863-1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be jo...

journal_title：Nucleic acids research

authors： Korch C

更新日期：1987-04-24 00:00:00

abstract：:We previously constructed a multiribozyme expression vector by combining cis- and trans-acting ribozymes and we showed that several ribozymes, each directed against a different target in the HIV genome and acting independently in a 'shotgun' manner, markedly increased the efficiency of cleavage of HIV RNA in vitro [Oh...

journal_title：Nucleic acids research

authors： Yuyama N,Ohkawa J,Koguma T,Shirai M,Taira K

更新日期：1994-11-25 00:00:00