Abstract:
:Evidence is presented that the editing mechanisms of aminoacyl-tRNA synthetase operate by two alternative pathways: pre-transfer, by hydrolysis of the non-cognate aminoacyl adenylate; post-transfer, by hydrolysis of the mischarged tRNA. The methionyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus and isoleucyl-tRNA synthetase from E. coli, for example, are shown to reject misactivated homocysteine rapidly by the pre-transfer route. A novel feature of this reaction is that homocysteine thiolactone is formed by the facile cyclisation of the homocysteinyl adenylate. Valyl-tRNA synthetases, on the other hand, reject the more readily activated non-cognate amino acids by primarily the post-transfer route. The features governing the choice of pathway are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Jakubowski H,Fersht ARdoi
10.1093/nar/9.13.3105subject
Has Abstractpub_date
1981-07-10 00:00:00pages
3105-17issue
13eissn
0305-1048issn
1362-4962journal_volume
9pub_type
杂志文章abstract::We have compiled the DNA sequence data for E. coli available from the GENBANK and EMBL data libraries and over a period of several years independently from the literature. This is the fifth listing replacing and increasing the former listings substantially. However, in order to save space this printed version contains...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.13.2973
更新日期:1993-07-01 00:00:00
abstract::We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished without the introduction ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.5.1445
更新日期:1978-05-01 00:00:00
abstract::Initially identified as a factor involved in tyrosine kinase receptor signaling, Grb10-interacting GYF protein 2 (GIGYF2) has later been shown to interact with the 5' cap-binding protein 4EHP as part of a translation repression complex, and to mediate post-transcriptional repression of tethered reporter mRNAs. A curre...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky198
更新日期:2018-06-20 00:00:00
abstract::We describe here a novel method for integrating gene and miRNA expression profiles in cancer using feed-forward loops (FFLs) consisting of transcription factors (TFs), miRNAs and their common target genes. The dChip-GemiNI (Gene and miRNA Network-based Integration) method statistically ranks computationally predicted ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks395
更新日期:2012-09-01 00:00:00
abstract::Bacteria from the genus Streptomyces are very important for the production of natural bioactive compounds such as antibiotic, antitumour or immunosuppressant drugs. Around two-thirds of all known natural antibiotics are produced by these bacteria. An enormous quantity of crucial data related to this genus has been gen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1253
更新日期:2013-01-01 00:00:00
abstract::Guanine-quadruplexes (G4) consist of non-canonical four-stranded helical arrangements of guanine-rich nucleic acid sequences. The bulky and thermodynamically stable features of G4 structures have been shown in many respects to affect normal nucleic acid metabolism. In vivo conversion of G4 structures to single-strande...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr630
更新日期:2011-11-01 00:00:00
abstract::RNA-protein complexes are essential in mediating important fundamental cellular processes, such as transport and localization. In particular, ncRNA-protein interactions play an important role in post-transcriptional gene regulation like mRNA localization, mRNA stabilization, poly-adenylation, splicing and translation....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv020
更新日期:2015-02-18 00:00:00
abstract::We used the high resolution and accuracy of the Cambridge Structural Database (CSD) to provide detailed information regarding base pairing interactions of selected nucleobases. We searched for base pairs in which nucleobases interact with each other through two or more hydrogen bonds and form more or less planar struc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa629
更新日期:2020-09-04 00:00:00
abstract::A number of regulatory nascent peptides have been shown to regulate gene expression by causing programmed ribosome stalling during translation. Nascent peptide emerges from the ribosome through the exit tunnel, and one-third of the way along which β-loop structures of ribosomal proteins uL4 and uL22 protrude into the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz1190
更新日期:2020-02-28 00:00:00
abstract::Data produced with short-read sequencing technologies result in ambiguous haplotyping and a limited capacity to investigate the full repertoire of biologically relevant forms of genetic variation. The notion of haplotype-resolved sequencing data has recently gained traction to reduce this unwanted ambiguity and enable...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx436
更新日期:2017-07-27 00:00:00
abstract::Single-nucleotide polymorphisms (SNPs) are often linked to critical phenotypes such as diseases or responses to vaccines, medications and environmental factors. However, the specific molecular mechanisms by which a causal SNP acts is usually not obvious. Changes in RNA secondary structure emerge as a possible explanat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1009
更新日期:2013-01-07 00:00:00
abstract::The RDM1 gene encodes a RNA recognition motif (RRM)-containing protein involved in the cellular response to the anti-cancer drug cisplatin in vertebrates. We previously reported a cDNA encoding the full-length human RDM1 protein. Here, we describe the identification of 11 human cDNAs encoding RDM1 protein isoforms. Th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm753
更新日期:2007-01-01 00:00:00
abstract::Deamination of cytosine (C), 5-methylcytosine (mC) and 5-hydroxymethylcytosine (hmC) occurs spontaneously in mammalian DNA with several hundred deaminations occurring in each cell every day. The resulting potentially mutagenic mispairs of uracil (U), thymine (T) or 5-hydroxymethyluracil (hmU) with guanine (G) are subs...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks259
更新日期:2012-07-01 00:00:00
abstract::In the last decade polycistronic vectors have become essential tools for both basic science and gene therapy applications. In order to co-express heterologous polypeptides, different systems have been developed from Internal Ribosome Entry Site (IRES) based vectors to the use of the 2A peptide. Unfortunately, these me...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp716
更新日期:2009-11-01 00:00:00
abstract::FASTQ has emerged as a common file format for sharing sequencing read data combining both the sequence and an associated per base quality score, despite lacking any formal definition to date, and existing in at least three incompatible variants. This article defines the FASTQ format, covering the original Sanger stand...
journal_title:Nucleic acids research
pub_type: 历史文章,杂志文章,评审
doi:10.1093/nar/gkp1137
更新日期:2010-04-01 00:00:00
abstract::We have isolated a pigment cell-specific cDNA clone from a B16 mouse melanoma cDNA library by differential hybridization. The mRNA of isolated cDNA is highly expressed in B16 melanoma cells and in black mouse (C57BL/6) skin, but is not detectable in mouse neuroblastoma cells nor in K1735 mouse amelanotic melanoma cell...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.6.2413
更新日期:1986-03-25 00:00:00
abstract::The oncoprotein c-Myc plays an important role in cell proliferation, transformation, inhibition of differentiation and apoptosis. These functions most likely result from the transcription factor activity of c-Myc. As a heterodimer with Max, the c-Myc protein binds to the E-box sequence (CACGTG), which is also recogniz...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.8.1493
更新日期:1997-04-15 00:00:00
abstract::KU is a heterodimer, consisting of approximately 70 and approximately 80 kDa subunits (KU70 and KU80, respectively), which is involved in a variety of nuclear functions. We generated tbKU80-deficient trypanosomes to explore the potential role of the tbKU complex in telomere maintenance and transcriptional regulation o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh991
更新日期:2004-12-15 00:00:00
abstract::Mutator-like transposable elements (MULEs) are widespread across fungal, plant and animal species. Despite their abundance and importance as genetic tools in plants, the transposition mechanism of the MULE superfamily was previously unknown. Discovery of the Muta1 element from Aedes aegypti and its successful transpos...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx357
更新日期:2017-06-20 00:00:00
abstract::The cis-requirements for the first editing site in the atp9 mRNA from pea mitochondria were investigated in an in vitro RNA editing system. Template RNAs deleted 5' of -20 are edited correctly, but with decreased efficiency. Deletions between -20 and the edited nucleotide abolish editing activity. Substitution of the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh763
更新日期:2004-08-04 00:00:00
abstract::The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stabili...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.6.759
更新日期:1975-06-01 00:00:00
abstract::The allele frequency net database (http://www.allelefrequencies.net) is an online repository that contains information on the frequencies of immune genes and their corresponding alleles in different populations. The extensive variability observed in genes and alleles related to the immune system response and its signi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1128
更新日期:2011-01-01 00:00:00
abstract::It has recently been demonstrated that nucleobase-density profiles of typical mRNA coding sequences exhibit a complementary relationship with nucleobase-interaction propensity profiles of their cognate protein sequences. This finding supports the idea that the genetic code developed in response to direct binding inter...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv166
更新日期:2015-03-31 00:00:00
abstract::The 'druggable genome' encompasses several protein families, but only a subset of targets within them have attracted significant research attention and thus have information about them publicly available. The Illuminating the Druggable Genome (IDG) program was initiated in 2014, has the goal of developing experimental...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1072
更新日期:2017-01-04 00:00:00
abstract::Myocyte enhancer binding factor 2 (MEF2) proteins belong to the MADS box family of transcription factors and four MEF2 proteins, MEF2A, MEF2B, MEF2C and MEF2D, have been found. MEF2 proteins have been shown to play critical roles in differentiation of muscles and neuronal tissues. How transactivational activity of MEF...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.13.2836
更新日期:2001-07-01 00:00:00
abstract::MetaNetX is a repository of genome-scale metabolic networks (GSMNs) and biochemical pathways from a number of major resources imported into a common namespace of chemical compounds, reactions, cellular compartments--namely MNXref--and proteins. The MetaNetX.org website (http://www.metanetx.org/) provides access to the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1117
更新日期:2016-01-04 00:00:00
abstract::UV-induced reversion of the arg4-17 ochre allele in Saccharomyces cerevisiae is largely dependent on translesion polymerase eta (Rad30p), known to bypass cyclobutane-type TT dimers in an error-free fashion. arg4-17 locus reversion was predominantly due to T-->C transition of T127, the 3' T of a TT photoproduct site. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/30.5.1262
更新日期:2002-03-01 00:00:00
abstract::We have explored the functional relationships between spliceosome and Microprocessor complex activities in a novel class of microRNAs (miRNAs), named Splice site Overlapping (SO) miRNAs, whose pri-miRNA hairpins overlap splice sites. We focused on the evolutionarily conserved SO miR-34b, and we identified two indispen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt614
更新日期:2013-10-01 00:00:00
abstract::Properties of a mutant bacteriophage T2 DNA [N:(6)-adenine] methyltransferase (T2 Dam MTase) have been investigated for its potential utilization in RecA-assisted restriction endonuclease (RARE) cleavage. Steady-state kinetic analyses with oligonucleotide duplexes revealed that, compared to wild-type T4 Dam, both wild...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.7.1484
更新日期:2001-04-01 00:00:00
abstract::This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the o...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.2.582
更新日期:2001-01-15 00:00:00