Abstract:
:Gene Ontology (GO) is the most widely accepted attempt to construct a unified and structured vocabulary for the description of genes and their products in any organism. Annotation by GO terms is performed in most of the current genome projects, which besides generality has the advantage of being very convenient for computer based classification methods. However, direct use of GO in small sequencing projects is not easy, especially for species not commonly represented in public databases. We present a software package (GOblet), which performs annotation based on GO terms for anonymous cDNA or protein sequences. It uses the species independent GO structure and vocabulary together with a series of protein databases collected from various sites, to perform a detailed GO annotation by sequence similarity searches. The sensitivity and the reference protein sets can be selected by the user. GOblet runs automatically and is available as a public service on our web server. The paper also addresses the reliability of automated GO annotations by using a reference set of more than 6000 human proteins. The GOblet server is accessible at http://goblet.molgen.mpg.de.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Hennig S,Groth D,Lehrach Hdoi
10.1093/nar/gkg582keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
3712-5issue
13eissn
0305-1048issn
1362-4962journal_volume
31pub_type
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