Abstract:
:We describe a simple procedure for photolithographic patterning of streptavidin on silicon substrates. Long wavelength UV (365 nm) light was used to direct the covalent attachment of photoactivatable biotin onto silylated silicon wafers. Fluorescently labeled streptavidin was found to bind only in areas exposed to the light. We used this procedure to selectively pattern streptavidin inside microwells etched in silicon, and we investigated the binding characteristics of biotinylated oligonucleotides of lengths, n = 16, 54 and 99 bases. The binding curves were found to fit the functional form of the Langmuir isotherm, with binding saturation proportional to n(-3/4).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Sabanayagam CR,Smith CL,Cantor CRdoi
10.1093/nar/28.8.e33keywords:
subject
Has Abstractpub_date
2000-04-15 00:00:00pages
E33issue
8eissn
0305-1048issn
1362-4962pii
gnd034journal_volume
28pub_type
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