Abstract:
:Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (GI) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Quejada JR,Park SE,Awari DW,Shi F,Yamamoto HE,Kawano F,Jung JC,Yazawa Mdoi
10.1093/nar/gkx804subject
Has Abstractpub_date
2017-11-16 00:00:00pages
e172issue
20eissn
0305-1048issn
1362-4962pii
4344570journal_volume
45pub_type
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