Abstract:
:The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Azuma Y,Yamagishi M,Ueshima R,Ishihama Adoi
10.1093/nar/19.3.461subject
Has Abstractpub_date
1991-02-11 00:00:00pages
461-8issue
3eissn
0305-1048issn
1362-4962journal_volume
19pub_type
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